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Volume 152, Issue 4

A synthetic promoter library for constitutive gene expression in Free

Abstract

A synthetic promoter library (SPL) for has been developed, which generalizes the approach for obtaining synthetic promoters. The consensus sequence, derived from rRNA promoters extracted from the WCFS1 genome, was kept constant, and the non-consensus sequences were randomized. Construction of the SPL was performed in a vector (pSIP409) previously developed for high-level, inducible gene expression in and . A wide range of promoter strengths was obtained with the approach, covering 3–4 logs of expression levels in small increments of activity. The SPL was evaluated for the ability to drive -glucuronidase (GusA) and aminopeptidase N (PepN) expression. Protein production from the synthetic promoters was constitutive, and the most potent promoters gave high protein production with levels comparable to those of native rRNA promoters, and production of PepN protein corresponding to approximately 10–15 % of the total cellular protein. High correlation was obtained between the activities of promoters when tested in and , which indicates the potential of the SPL for other species. The SPL enables fine-tuning of stable gene expression for various applications in .

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Keyword(s): LAB, lactic acid bacteria and SPL, synthetic promoter library
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2006-04-01
2024-04-23
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A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum
Microbiology 152, 1011 (2006); https://doi.org/10.1099/mic.0.28599-0
/content/journal/micro/10.1099/mic.0.28599-0
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