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Volume 152, Issue 4

Demonstration of regulatory cross-talk between P fimbriae and type 1 fimbriae in uropathogenic Free

Abstract

The majority of strains isolated from urinary tract infections have the potential to express multiple fimbriae. Two of the most common fimbrial adhesins are type 1 fimbriae and pyelonephritis-associated pili (Pap). Previous research has shown that induced, plasmid-based expression of a Pap regulator, , and its close homologues can prevent inversion of the switch controlling the expression of type 1 fimbriae. The aim of the present study was to determine if this cross-regulation occurs when PapB is expressed from its native promoter in the chromosome of K-12 and clinical isolates. The regulation was examined in three ways: (1) mutated alleles of the regulatory region, including and , that maintain the promoter in either the off or the on phase were exchanged into the chromosome of both K-12 and the clinical isolate CFT073, and the effect on type 1 fimbrial expression was measured; (2) type 1 fimbrial expression was determined using a novel  : :  reporter system in mutants of the clinical isolate 536 in which combinations of complete fimbrial clusters had been deleted; (3) type 1 fimbrial expression was determined in a range of clinical isolates and compared with both the number of P clusters and their expression. All three approaches demonstrated that P expression represses type 1 fimbrial expression. Using a number of novel genetic approaches, this work extends the initial finding that PapB inhibits FimB recombination to the impact of this regulation in clinical isolates.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): GFP, green fluorescent protein , HA, haemagglutination , Lrp, leucine-responsive regulatory protein , MRHA, mannose-resistant HA , MSHA, mannose-sensitive HA , Pap, pyelonephritis-associated pili , Prf, Pap-related fimbriae , RBC, red blood cell , Sfa, S-fimbrial adhesin , UPEC, uropathogenic E. coli and UTI, urinary tract infection
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2006-04-01
2024-04-20
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Demonstration of regulatory cross-talk between P fimbriae and type 1 fimbriae in uropathogenic Escherichia coli
Microbiology 152, 1143 (2006); https://doi.org/10.1099/mic.0.28677-0
/content/journal/micro/10.1099/mic.0.28677-0
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