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Volume 152, Issue 8

Morphological changes and proteome response of to a partial depletion of FtsI Free

Abstract

In , as in many Gram-positive bacteria, the cell division gene is located at the beginning of the cluster, which comprises cell division- and cell wall-related genes. Transcriptional analysis of the cluster revealed that is transcribed as part of a polycistronic mRNA, which includes at least , , , and , from a promoter that is located upstream of . appears also to be expressed from a minor promoter that is located in the intergenic region. It is an essential gene in , and a reduced expression of leads to the formation of larger and filamentous cells. A translational GFP-FtsI fusion protein was found to be functional and localized to the mid-cell of a growing bacterium, providing evidence of its role in cell division in . This study involving proteomic analysis (using 2D SDS-PAGE) of a strain that has partially depleted levels of FtsI reveals that at least 20 different proteins were overexpressed in the organism. Eight of these overexpressed proteins, which include DivIVA, were identified by MALDI-TOF. Overexpression of DivIVA was confirmed by Western blotting using anti-DivIVA antibodies, and also by fluorescence microscopy analysis of a RESF1 strain expressing a chromosomal copy of a transcriptional fusion. Overexpression of DivIVA was not observed when FtsI was inhibited by cephalexin treatment or by partial depletion of FtsZ.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Q-PCR, quantitative PCR and RACE, rapid amplification of cDNA ends
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2006-08-01
2024-04-25
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Morphological changes and proteome response of Corynebacterium glutamicum to a partial depletion of FtsI
Microbiology 152, 2491 (2006); https://doi.org/10.1099/mic.0.28773-0
/content/journal/micro/10.1099/mic.0.28773-0
/content/journal/micro/10.1099/mic.0.28773-0
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