1887

Abstract

Adenylyltransferase, GlnE, has a predicted role in controlling the enzymic activity of glutamine synthetase, the key enzyme in ammonia assimilation. It was previously demonstrated that is an essential gene in . is located downstream of , one of four glutamine synthetases. The expression of GlnE under various conditions was determined. Although a co-transcript of and was detectable, the major transcript was monocistronic. A transcriptional start site immediately upstream of was identified and it was shown by site-directed mutagenesis that the predicted −10 region is a functional promoter. It was demonstrated that in a background P was up-regulated in ammonia- or glutamine-containing media.

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2006-09-01
2024-04-19
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References

  1. Agarwal N, Tyagi A. K. 2003; Role of 5′-TGN-3′ motif in the interaction of mycobacterial RNA polymerase with a promoter of ‘extended −10’ class. FEMS Microbiol Lett 225:75–83 [CrossRef]
    [Google Scholar]
  2. Bashyam M. D, Tyagi A. K. 1998; Identification and analysis of “extended −10” promoters from mycobacteria. J Bacteriol 180:2568–2573
    [Google Scholar]
  3. Betts J. C, Lukey P. T, Robb L. C, McAdam R. A, Duncan K. 2002; Evaluation of a nutrient starvation model of Mycobacterium tuberculosis persistence by gene and protein expression profiling. Mol Microbiol 43:717–731 [CrossRef]
    [Google Scholar]
  4. Cole S. T, Brosch R, Parkhill J. 39 other authors 1998; Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537–544 [CrossRef]
    [Google Scholar]
  5. Collins D. M, Wilson T, Campbell S, Buddle B. M, Wards B. J, Hotter G, de Lisle G. W. 2002; Production of avirulent mutants of Mycobacterium bovis with vaccine properties by the use of illegitimate recombination and screening of stationary-phase cultures. Microbiology 148:3019–3027
    [Google Scholar]
  6. Dussurget O, Timm J, Gomez M, Gold B, Yu S. W, Sabol S. Z, Holmes R. K, Jacobs W. R, Smith I. 1999; Transcriptional control of the iron-responsive fxbA gene by the mycobacterial regulator IdeR. J Bacteriol 181:3402–3408
    [Google Scholar]
  7. Fink D, Falke D, Wohlleben W, Engels A. 1999; Nitrogen metabolism in Streptomyces coelicolor A3(2): modification of glutamine synthetase I by an adenylyltransferase. Microbiology 145:2313–2322
    [Google Scholar]
  8. Fink D, Weissschuh N, Reuther J, Wohlleben W, Engels A. 2002; Two transcriptional regulators GlnR and GlnRII are involved in regulation of nitrogen metabolism in Streptomyces coelicolor A3(2). Mol Microbiol 46:331–347 [CrossRef]
    [Google Scholar]
  9. Harth G, Horwitz M. A. 1999; An inhibitor of exported Mycobacterium tuberculosis glutamine synthetase selectively blocks the growth of pathogenic mycobacteria in axenic culture and in human monocytes: extracellular proteins as potential novel drug targets. J Exp Med 189:1425–1435 [CrossRef]
    [Google Scholar]
  10. Harth G, Clemens D. L, Horwitz M. A. 1994; Glutamine synthetase of Mycobacterium tuberculosis : extracellular release and characterization of its enzymatic activity. Proc Natl Acad Sci U S A 91:9342–9346 [CrossRef]
    [Google Scholar]
  11. Harth G, Zamecnik P. C, Tang J. Y, Tabatadze D, Horwitz M. A. 2000; Treatment of Mycobacterium tuberculosis with antisense oligonucleotides to glutamine synthetase mRNA inhibits glutamine synthetase activity, formation of the poly-l-glutamate/glutamine cell wall structure, and bacterial replication. Proc Natl Acad Sci U S A 97:418–423 [CrossRef]
    [Google Scholar]
  12. Harth G, Maslesa-Galic S, Tullius M. V, Horwitz M. A. 2005; All four Mycobacterium tuberculosis glnA genes encode glutamine synthetase activities but only GlnA1 is abundantly expressed and essential for bacterial homeostasis. Mol Microbiol 58:1157–1172 [CrossRef]
    [Google Scholar]
  13. Jakoby M, Nolden L, Meier-Wagner J, Kramer R, Burkovski A. 2000; AmtR, a global repressor in the nitrogen regulation system of Corynebacterium glutamicum . Mol Microbiol 37:964–977 [CrossRef]
    [Google Scholar]
  14. Kamalakannan V, Ramachandran G, Narayanan S, Vasan S. K, Narayanan P. R. 2002; Identification of a novel mycobacterial transcriptional regulator and its involvement in growth rate dependence and stringent control. FEMS Microbiol Lett 209:261–266 [CrossRef]
    [Google Scholar]
  15. Manganelli R, Dubnau E, Tyagi S, Kramer F. R, Smith I. 1999; Differential expression of 10 sigma factor genes in Mycobacterium tuberculosis . Mol Microbiol 31:715–724 [CrossRef]
    [Google Scholar]
  16. Merrick M. J, Edwards R. A. 1995; Nitrogen control in bacteria. Microbiol Rev 59:604–622
    [Google Scholar]
  17. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Nolden L, Farwick M, Kramer R, Burkovski A. 2001; Glutamine synthetases of Corynebacterium glutamicum : transcriptional control and regulation of activity. FEMS Microbiol Lett 201:91–98 [CrossRef]
    [Google Scholar]
  19. Parish T, Stoker N. G. 2000; glnE is an essential gene in Mycobacterium tuberculosis . J Bacteriol 182:5715–5720 [CrossRef]
    [Google Scholar]
  20. Parish T, Lewis J, Stoker N. G. 2001; Use of the mycobacteriophage L5 excisionase in Mycobacterium tuberculosis to demonstrate gene essentiality. Tuberculosis 81:359–364 [CrossRef]
    [Google Scholar]
  21. Reitzer L. J, Magasanik B. others 1987; Ammonia assimilation and the biosynthesis of glutamine, glutamate, asparagine, l-alanine and d-alanine. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology Edited by Neidhart F. C. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Timm J, Perilli M. G, Duez C. 7 other authors 1994; Transcription and expression analysis, using lacZ and phoA gene fusions, of Mycobacterium fortuitum beta-lactamase genes cloned from a natural isolate and a high-level beta-lactamase producer. Mol Microbiol 12:491–504 [CrossRef]
    [Google Scholar]
  23. Tullius M. V, Harth G, Horwitz M. A. 2001; High extracellular levels of Mycobacterium tuberculosis glutamine synthetase and superoxide dismutase in actively growing cultures are due to high expression and extracellular stability rather than to a protein-specific export mechanism. Infect Immun 69:6348–6363 [CrossRef]
    [Google Scholar]
  24. Tullius M. V, Harth G, Horwitz M. A. 2003; Glutamine synthetase GlnA1 is essential for growth of Mycobacterium tuberculosis in human THP-1 macrophages and guinea pigs. Infect Immun 71:3927–3936 [CrossRef]
    [Google Scholar]
  25. Wernisch L, Kendall S. L, Soneji S, Wietzorrek A, Parish T, Hinds J, Butcher P. D, Stoker N. G. 2003; Analysis of whole-genome microarray replicates using mixed models. Bioinformatics 19:53–61 [CrossRef]
    [Google Scholar]
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