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Volume 152, Issue 10

Identification and characterization of membrane transporters necessary for utilization of the siderophore pyridine-2,6-bis(thiocarboxylic acid) (PDTC) Free

Abstract

The compound pyridine-2,6-bis(thiocarboxylic acid) (PDTC) is known to be produced and excreted by three strains of . Its reactivity includes the complete dechlorination of the environmental contaminant carbon tetrachloride. PDTC functions as a siderophore; however, roles as a ferric reductant and antimicrobial agent have also been proposed. PDTC function and regulation were further explored by characterizing the phenotypes of mutants in predicted membrane transporter genes. The functions of a predicted outer-membrane transporter (PdtK) and a predicted inner-membrane permease (PdtE) were examined in DSM 3601. Uptake of iron from Fe(III):PDTC, and bioutilization of PDTC in a chelated medium, were dependent upon PdtK and PdtE. Another strain of (KT2440), which lacks orthologues, showed growth inhibition by PDTC that could be relieved by introducing a plasmid containing KCPE. Transcriptional activation in response to exogenously added PDTC (25 μM) was unaltered by the K or E mutations; each mutant showed activation of a transcriptional reporter, indistinguishable from an isogenic PDTC utilization-proficient strain. The data demonstrate that PdtK and PdtE constitute a bipartite outer-membrane/inner-membrane transport system for iron acquisition from Fe(III):PDTC. Disruptions in this portion of the DSM 3601 gene cluster do not abolish PDTC-dependent transcriptional signalling.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Cm, chloramphenicol , CT, carbon tetrachloride , Fur, ferric uptake regulator , Gm, gentamicin , Km, kanamycin , o-p, 1,10-phenanthroline , PDTC, pyridine-2,6-bis(thiocarboxylic acid) and Tc, tetracycline
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2006-10-01
2024-04-23
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Identification and characterization of Pseudomonas membrane transporters necessary for utilization of the siderophore pyridine-2,6-bis(thiocarboxylic acid) (PDTC)
Microbiology 152, 3157 (2006); https://doi.org/10.1099/mic.0.29116-0
/content/journal/micro/10.1099/mic.0.29116-0
/content/journal/micro/10.1099/mic.0.29116-0
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