1887

Abstract

Despite accumulating data implicating in a variety of diseases, its precise role in infection remains to be determined. antigen-specific CD4 T cells are present in early inflamed acne lesions and may be involved in the inflammatory response; however, little is known about the specific antigens involved. In this study, B cell and T cell antigens from expression libraries were cloned and evaluated and the four predominant proteins identified were investigated. Two of these antigens share some homology with an M-like protein of and have dermatan-sulphate-binding activity (PA-25957 and 5541). The remaining two antigens, PA-21693 and 4687, are similar to the product of the gene from the ABC transport locus, although only one of these (PA-21693) is encoded within an -like operon and conserved amongst a range of clinical isolates. All four proteins contain an LPXTG motif, although only PA-21693 contains a characteristic sortase-sorting signal. Variation in the expression of PA-4687, 25957 and 5541 is evident amongst clinical isolates and is generated both by frameshifts associated with the putative signal peptide and by variable numbers of repeat regions toward the carboxy-terminus, potentially generating heterogeneity of molecular mass and antigenic variation. In addition, in the case of PA-25957, a frameshift in a C-rich region at the extreme carboxy-terminus eliminates the LPXTG motif in some isolates. For the dermatan-sulphate-binding PA-25957, IgG1 antibody in serum from acne-positive donors was shown to be specific for the amino-terminal region of the protein, which also contains a CD4 T cell epitope. In contrast, serum from acne-negative donors shows an IgG2 and IgG3 antibody subclass response to the carboxy-terminal region. These data have implications for the potential role of in inflammatory acne and other diseases.

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2006-12-01
2024-03-29
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References

  1. Bendtsen J. D, Nielsen H, Brunak S, von Heijne G. 2004; Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 340:783–795 [CrossRef]
    [Google Scholar]
  2. Bojar R. A, Holland K. T. 2004; Acne and Propionibacterium acnes . Clin Dermatol 22:375–379 [CrossRef]
    [Google Scholar]
  3. Braga E. G, Ananias R. Z, Mussalem J. S, Squaiella C. C, Longhini A. L, Mariano M, Travassos L. R, Longo-Maugeri I. M. 2003; Treatment with Propionibacterium acnes modulates the late phase reaction of immediate hypersensitivity in mice. Immunol Lett 88:163–169 [CrossRef]
    [Google Scholar]
  4. Brook I. 2002; Endocarditis due to anaerobic bacteria. Cardiology 98:1–5 [CrossRef]
    [Google Scholar]
  5. Bruggemann H, Henne A, Hoster F, Liesegang H, Wiezer A, Strittmatter A, Hujer S, Durre P, Gottschalk G. 2004; The complete genome sequence of Propionibacterium acnes , a commensal of human skin. Science 305:671–673 [CrossRef]
    [Google Scholar]
  6. Clamp M, Cuff J, Searle S. M, Barton G. J. 2004; The Jalview Java alignment editor. Bioinformatics 20:426–427 [CrossRef]
    [Google Scholar]
  7. Cove J. H, Holland K. T, Cunliffe W. J. 1983; Effects of oxygen concentration on biomass production, maximum specific growth rate and extracellular enzyme production by three species of cutaneous propionibacteria grown in continuous culture. J Gen Microbiol 129:3327–3334
    [Google Scholar]
  8. Davies M. 1982; Bacterial cells as anti-tumour agents in man. Rev Environ Health 4:31–56
    [Google Scholar]
  9. Eady E. A, Cove J. H, Holland K. T, Cunliffe W. J. 1989; Erythromycin resistant propionibacteria in antibiotic treated acne patients: association with therapeutic failure. Br J Dermatol 121:51–57
    [Google Scholar]
  10. Eady E. A, Gloor M, Leyden J. J. 2003; Propionibacterium acnes resistance: a worldwide problem. Dermatology 206:54–56 [CrossRef]
    [Google Scholar]
  11. Farrar M. D, Ingham E. 2004; Acne: inflammation. Clin Dermatol 22:380–384 [CrossRef]
    [Google Scholar]
  12. Frick I. M, Schmidtchen A, Sjobring U. 2003; Interactions between M proteins of Streptococcus pyogenes and glycosaminoglycans promote bacterial adhesion to host cells. Eur J Biochem 270:2303–2311 [CrossRef]
    [Google Scholar]
  13. Gao T. W, Li C. Y, Zhao X. D, Liu Y. F. 2002; Fatal bacteria granuloma after trauma: a new entity. Br J Dermatol 147:985–993 [CrossRef]
    [Google Scholar]
  14. Gribbon E. M, Cunliffe W. J, Holland K. T. 1993; Interaction of Propionibacterium acnes with skin lipids in vitro . J Gen Microbiol 139:1745–1751 [CrossRef]
    [Google Scholar]
  15. Holland K. T, Holland D. B, Cunliffe W. J, Cutcliffe A. G. 1993; Detection of Propionibacterium acnes polypeptides which have stimulated an immune response in acne patients but not in normal individuals. Exp Dermatol 2:12–16 [CrossRef]
    [Google Scholar]
  16. Ingham E. 1999; The immunology of Propionibacterium acnes and acne. Curr Opin Infect Dis 12:191–197 [CrossRef]
    [Google Scholar]
  17. Jappe U, Ingham E, Henwood J, Holland K. T. 2002; Propionibacterium acnes and inflammation in acne; P. acnes has T-cell mitogenic activity. Br J Dermatol 146:202–209 [CrossRef]
    [Google Scholar]
  18. Johnson J. L, Cummins C. S. 1972; Cell wall composition and deoxyribonucleic acid similarities among anaerobic coryneforms, classical propionibacteria, and strains of Arachnia propionica . J Bacteriol 109:1047–1066
    [Google Scholar]
  19. Jousimies-Somer H. R, Summanen P, Citron D. M, Baron E. J, Wexler H. M, Finegold S. M. 2002 Wadsworth-KTL Anaerobic Bacteriology Manual , 6th edn. Belmont: Star Publishing Co;
  20. Kim J, Ochoa M.-T, Krutzik S. R. 10 other authors 2002; Activation of Toll-like receptor 2 in acne triggers inflammatory cytokine responses. J Immunol 169:1535–1541 [CrossRef]
    [Google Scholar]
  21. Kirchhoff T, Merkesdal S, Rosenthal H. 7 other authors 2003; Diagnostic management of patients with SAPHO syndrome: use of MR imaging to guide bone biopsy at CT for microbiological and histological work-up. Eur Radiol 13:2304–2308 [CrossRef]
    [Google Scholar]
  22. Koreck A, Pivarcsi A, Dobozy A, Kemeny L. 2003; The role of innate immunity in the pathogenesis of acne. Dermatology 206:96–105 [CrossRef]
    [Google Scholar]
  23. Lee S. G, Pancholi V, Fischetti V. A. 2002; Characterization of a unique glycosylated anchor endopeptidase that cleaves the LPXTG sequence motif of cell surface proteins of Gram-positive bacteria. J Biol Chem 277:46912–46922 [CrossRef]
    [Google Scholar]
  24. Leyden J. J, McGinley K. J, Vowels B. 1998; Propionibacterium acnes colonization in acne and nonacne. Dermatology 196:55–58 [CrossRef]
    [Google Scholar]
  25. Lodes M. J, Cong Y, Elson C. O, Mohamath R, Landers C. J, Targan S. R, Fort M, Hershberg R. M. 2004a; Bacterial flagellin is a dominant antigen in Crohn's disease. J Clin Invest 113:1296–1306 [CrossRef]
    [Google Scholar]
  26. Lodes M. J, Dillon D. C, Houghton R. L, Skeiky Y. A. W. 2004b; Expression cloning. In Molecular Diagnosis of Infectious Diseases , 2nd edn. pp.  91–106 Edited by Decker J., Reisch U. New Jersey: Humana Press;
    [Google Scholar]
  27. Matsui K, Yoshimoto T, Tsutsui H. 7 other authors 1997; Propionibacterium acnes treatment diminishes CD4+NK1.1+ T cells but induces type I T-cells in the liver by induction of IL-12 and IL-18 production from Kupffer cells. J Immunol 159:97–106
    [Google Scholar]
  28. Moller D. R, Chen E. S. 2002; What causes sarcoidosis?. Curr Opin Pulm Med 8:429–434 [CrossRef]
    [Google Scholar]
  29. Mouser P. E, Baker B. S, Seaton E. D, Chu A. C. 2003; Propionibacterium acnes -reactive T helper-1 cells in the skin of patients with acne vulgaris. J Invest Dermatol 121:1226–1228 [CrossRef]
    [Google Scholar]
  30. Mrabet-Dahbi S, Breuer K, Klotz M, Herz U, Heeg K, Werfel T, Renz H. 2005; Deficiency in immunoglobulin G2 antibodies against staphylococcal enterotoxin C1 defines a subgroup of patients with atopic dermatitis. Clin Exp Allergy 35:274–281 [CrossRef]
    [Google Scholar]
  31. Nagy I, Pivarcsi A, Koreck A, Szell M, Urban E, Kemeny L. 2005; Distinct strains of Propionibacterium acnes induce selective human beta-defensin-2 and interleukin-8 expression in human keratinocytes through toll-like receptors. J Invest Dermatol 124:931–938 [CrossRef]
    [Google Scholar]
  32. Nagy I, Pivarcsi A, Kis S. 7 other authors 2006; Propionibacterium acnes and lipopolysaccharide induce the expression of antimicrobial peptides and proinflammatory cytokines/chemokines in human sebocytes. Microbes Infect 8:2195–2205 [CrossRef]
    [Google Scholar]
  33. Pallen M. J, Lam A. C, Antonio M, Dunbar K. 2001; An embarrassment of sortases – a richness of substrates?. Trends Microbiol 9:97–102 [CrossRef]
    [Google Scholar]
  34. Proft T, Hilbert H, Layh-Schmitt G, Herrmann R. 1995; The proline-rich P65 protein of Mycoplasma pneumoniae is a component of the Triton X-100-insoluble fraction and exhibits size polymorphism in the strains M129 and FH. J Bacteriol 177:3370–3378
    [Google Scholar]
  35. Qian Y, Lee J. H, Holmes R. K. 2002; Identification of a DtxR-regulated operon that is essential for siderophore-dependent iron uptake in Corynebacterium diphtheriae . J Bacteriol 184:4846–4856 [CrossRef]
    [Google Scholar]
  36. Rodriguez G. M, Voskuil M. I, Gold B, Schoolnik G. K, Smith I. 2002; ideR , an essential gene in Mycobacterium tuberculosi s: role of IdeR in iron-dependent gene expression, iron metabolism, and oxidative stress response. Infect Immun 70:3371–3381 [CrossRef]
    [Google Scholar]
  37. Schmitt M. P, Drazek E. S. 2001; Construction and consequences of directed mutations affecting the hemin receptor in pathogenic Corynebacterium species. J Bacteriol 183:1476–1481 [CrossRef]
    [Google Scholar]
  38. Schneewind O, Fowler A, Faull K. F. 1995; Structure of the cell wall anchor of surface proteins in Staphylococcus aureus . Science 268:103–106 [CrossRef]
    [Google Scholar]
  39. Smith K. M, Brewer J. M, Rush C. M, Riley J, Garside P. 2004; In vivo generated Th1 cells can migrate to B cell follicles to support B cell responses. J Immunol 173:1640–1646 [CrossRef]
    [Google Scholar]
  40. Targan S. R, Landers C. J, Yang H, Lodes M. J, Cong Y, Papadakis K. A, Vasiliauskas E, Elson C. O, Hershberg R. M. 2005; Antibodies to CBir1 flagellin define a unique response that is associated independently with complicated Crohn's disease. Gastroenterology 128:2020–2028 [CrossRef]
    [Google Scholar]
  41. Thompson J. D, Higgins D. G, Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [CrossRef]
    [Google Scholar]
  42. Tunney M. M, Patrick S, Gorman S. P, Nixon J. R, Anderson N, Davis R. I, Hanna D, Ramage G. 1998; Improved detection of prosthetic hip infection: a currently underestimated problem. J Bone Joint Surg 80-B:568–572
    [Google Scholar]
  43. Tunney M. M, Patrick S, Curran M. D, Ramage G, Hanna D, Nixon J. R, Gorman S. P, Davis R. I, Anderson N. 1999; Detection of prosthetic hip infection at revision arthroplasty by immunofluorescence microscopy and PCR amplification of the bacterial 16S rRNA gene. J Clin Microbiol 37:3281–3290
    [Google Scholar]
  44. Valanne S, McDowell A, Ramage G. 10 other authors 2005; CAMP factor homologues in Propionibacterium acnes : a new protein family differentially expressed by types I and II. Microbiology 151:1369–1379 [CrossRef]
    [Google Scholar]
  45. van der Woude M. W, Baumler A. J. 2004; Phase and antigenic variation in bacteria. Clin Microbiol Rev 17:581–611 [CrossRef]
    [Google Scholar]
  46. Wong T, Ormonde S, Gamble G, McGhee C. N. 2003; Severe infective keratitis leading to hospital admission in New Zealand. Br J Ophthalmol 87:1103–1108 [CrossRef]
    [Google Scholar]
  47. Zedtwitz-Liebenstein K, Gabriel H, Graninger W. 2003; Pacemaker endocarditis due to Propionibacterium acnes . Infection 31:184–185
    [Google Scholar]
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