1887

Abstract

SUMMARY: Radiopenicillin is strongly bound by ultra-microscopic lipid-containing particles liberated on mechanical rupture of cells. The binding resembles that of intact cells in that it is irreversible and only occurs to a limited extent, but differs in that 7–12 times as much penicillin is bound per unit dry weight of material. The supernatant after centrifuging down the lipid particles decreases the titre of added penicillin as indicated by diffusion assay, possibly by a small irreversible inactivation superimposed upon a ‘reversible’ type of binding.

There is a correlation between the distribution of () the penicillin-binding component (), () S from radiopenicillin pretreated cells, and () lipid phosphorus, in the three fractions produced on rupture either in distilled water or in a formaldehyde solution. Rupture in formalin appears to allow the cells walls to retain most of the lipid particles and but only a little extra of the dry weight of the cells. Thus penicillin reacts with a lipid-containing fraction close to the cell wall in intact organisms. At least as much more is liberated on rupture of the cells as was available to the penicillin in the intact cell, but is somewhat unstable after rupture. These data are discussed in the light of evidence in the literature that penicillin may react initially with the osmotic barrier of bacteria.

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1954-04-01
2024-05-03
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References

  1. Bellamy W. D., Klimek J. W. 1948; The relation between induced resistance to penicillin and oxygen utilisation. J. Bact 55:147
    [Google Scholar]
  2. Bisset K. A. 1950 The Cytology and Life-History of Bacteria Edinburgh: E. and S. Livingstone;
    [Google Scholar]
  3. Braun W., Kraft M., Mead D. D., Goodlow R. J. 1952; The effect of penicillin on genetic changes and temporary modifications in populations of Brucellae. J. Bact 64:41
    [Google Scholar]
  4. Cooper P. D. 1953; The study of cell rupture in Staphylococcus aureus. J. gen. Microbiol 9:199
    [Google Scholar]
  5. Cooper P. D. 1954; The site of action of penicillin. III. Effect of surface active substances on penicillin uptake of Staph, aureus. Biochim. Biophys. Acta in the Press
    [Google Scholar]
  6. Cooper P. D., Clowes R. C., Rowley D. 1954; A note on the use of radioactive penicillin. J. gen. Microbiol 10:246
    [Google Scholar]
  7. Cooper P. D., Rowley D., Dawson I. M. 1949; Location of radioactive penicillin in Staph, aureus after contact with the drug. Nature; Lond: 164842
    [Google Scholar]
  8. Elford W. J. 1948; The influence of antibacterial substances on the interaction of bacteria and bacteriophages. 1. The influence of penicillin. J. gen. Microbiol 2:205
    [Google Scholar]
  9. Few A. V., Cooper P. D., Rowley D. 1952; Reaction of penicillin with the staphylococcal cell wall. Nature; Lond: 169283
    [Google Scholar]
  10. Few A. V., Cooper P. D., Rowley D. 1953; Determination of radiopenicillin uptake by electrodialysis. Biochem. J 53:xix
    [Google Scholar]
  11. Fiske C. H., SubbaRow Y. 1925; The colorimetric determination of phosphorus. J. biol. Chem 66:375
    [Google Scholar]
  12. Gale E. F. 1947; Correlation between penicillin resistance and assimilation affinity in Staph, aureus. Nature; Lond: 160407
    [Google Scholar]
  13. Gale E. F., Paine T. F. 1951; The assimilation of amino-acids by bacteria. 12. The action of inhibitors and antibiotics on the accumulation of free glutamic acid and the formation of combined glutamate in Staph, aureus. Biochem. J 48:298
    [Google Scholar]
  14. Gale E. F., Rodwell A. W. 1948; Amino-acid metabolism of penicillin-resistant staphylococci. J. Bact 55:161
    [Google Scholar]
  15. Gale E. F., Taylor E. S. 1947; The assimilation of amino-acids by bacteria. 5. The action of penicillin in preventing the assimilation of glutamic acid by Staph, aureus. J. gen. Microbiol 1:314
    [Google Scholar]
  16. Hotchkiss R. D. 1950; The abnormal course of bacterial protein synthesis in the presence of penicillin. J. exp. Med 91:351
    [Google Scholar]
  17. Hunter T. H., Baker K. T. 1949; The action of penicillin on Bacillus subtilis growing in the absence of amino acids. Science 110:423
    [Google Scholar]
  18. Klimek J. W., Cavallito C. J., Bailey J. H. 1948; Induced resistance of Staph, aureus to various antibiotics. J. Bact 55:139
    [Google Scholar]
  19. Klotz I. M., Urquhart J. M., Weber W. W. 1950; Penicillin-protein complexes. Arch. Biochem 26:420
    [Google Scholar]
  20. Maass E. A., Johnson M. J. 1949a; Penicillin uptake by bacterial cells. J. Bact 57:415
    [Google Scholar]
  21. Maass E. A., Johnson M. J. 1949b; The relations between bound penicillin and growth in Staphylococcus aureus. J. Bact 58:361
    [Google Scholar]
  22. McQuillen K. 1950-1; The bacterial surface. III. Effect of streptomycin on electrophoretic mobility of E. coli and Staph, aureus. Biochim. Biophys. Acta 7:54
    [Google Scholar]
  23. McQuillen K. 1951; The bacterial surface. IV. Effect of streptomycin on electrophoretic mobility of E. coli and Staph, aureus. Biochim. Biophys. Acta 7:54
    [Google Scholar]
  24. Mickle H. 1948; Tissue disintegration. J.R. micr. Soc 68:10
    [Google Scholar]
  25. Mitchell P. 1949a; The osmotic barrier in bacteria. In The Nature of the Bacterial Surface Symp. Soc. gen. Microbiol. 1:55
    [Google Scholar]
  26. Mitchell P. 1949b; Some observations on the mode of action of penicillin. Nature; Lond: 164259
    [Google Scholar]
  27. Mitchell P., Moyle J. 1950; Occurrence of a phosphoric ester in certain bacteria: its relation to Gram staining and penicillin sensitivity. Nature; Lond: 166218
    [Google Scholar]
  28. Mitchell P., Moyle J. 1951a; Relationships between cell growth, surface properties and nucleic acid production in normal and penicillin-treated Micrococcus pyogenes. J. gen. Microbiol 5:421
    [Google Scholar]
  29. Mitchell P., Moyle J. 1951b; The glycerophospho-protein complex envelope of Micrococcus pyogenes. J. gen. Microbiol 5:981
    [Google Scholar]
  30. Park J. T., Johnson M. J. 1949; Accumulation of labile phosphate in Staph, aureus grown in the presence of penicillin. J. biol. Chem 179:585
    [Google Scholar]
  31. Rowley D., Cooper P. D., Roberts P. W., Lester Smith E. 1950; The site of action of penicillin. 1. Uptake of penicillin on bacteria. Biochem. J 46:157
    [Google Scholar]
  32. Sobin B., Stahly G. L. 1942; The isolation and absorption spectrum maxima of bacterial carotenoid pigments. J. Bact 44:265
    [Google Scholar]
  33. Welch H. 1948; Cup-plate assay of penicillin concentrations in plasma-S. lutea. Meth. med. Res 1:25
    [Google Scholar]
  34. Work T. S., Work E. 1948 The Basis of Chemotherapy p. 234 Edinburgh and London: Oliver and Boyd;
    [Google Scholar]
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