1887

Abstract

SUMMARY: Staphylococcal bacteriophage K was concentrated and purified from broth lysates without the use of high-speed centrifugation, which inactivates this phage. After precipitation with 30 % (v/v) acetone in water in the cold, the phage was further purified by treatment with butanol and chloroform, and concentrated by pressure filtration in reinforced collodion sacs. Electron microscopical examination revealed the presence of empty phage heads and tails, in addition to intact phage in the purified concentrate. Experiments indicate that while these entities could be made by disintegration of the phage, they were already present in this form in the original lysate. Study of the structure of the phage indicates the presence of a removable solid rod or plug within the tail which terminates in the terminal bob. These findings are discussed in relation to the structure of other phages, and their physiology.

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/content/journal/micro/10.1099/00221287-10-2-250
1954-04-01
2024-05-03
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References

  1. Backus R. C., Williams R. C. 1950; The use of spraying methods and volatile suspending media in the preparation of specimens for electron microscopy. J. appl. Physiol 21:11
    [Google Scholar]
  2. Bloch H. 1940; Über einen Potenzierungseffekt bei Bakteriophagen. Arch. ges. Virusforsch 1:560
    [Google Scholar]
  3. Bronfenbrenner J. J., Korb C. 1925; Studies on the bacteriophage of D’Herelle. II. Effect of alcohol on the bacteriophage of D’Herelle. J. exp. Med 42:419
    [Google Scholar]
  4. Clifton C. E. 1930; A method for the purification of the bacteriophage. Proc. Soc. exp. Biol., N.Y 28:32
    [Google Scholar]
  5. Colwell C. A. 1937; Purified bacteriophage from lysogenic cultures. Proc. Soc. exp. Biol., N.Y 36:100
    [Google Scholar]
  6. Craig L. H. 1949; Studies on polypeptides and amino acids by countercurrent distribution. Cold Spr. Harb. Sym. quant. Biol 14:24
    [Google Scholar]
  7. Elford W. J. 1938; The sizes of viruses and bacteriophages and methods for their determination. In Handbuch der Virusforschung Doerr R., Hallauer C. ed pp. 126–76 Vienna: Julius Springer;
    [Google Scholar]
  8. Ende M. van den, Don P. A., Elford W. J., Challice C. E., Dawson I. M., Hotchin J. E. 1952; The bacteriophages of Pseudomonas aeruginosa filtration measurements and electron microscopy. J. Hyg., Camb 50:12
    [Google Scholar]
  9. Hedén C. G. 1951; Studies of the infection of E. coli B with the bacteriophage T2. Acta path, microbiol. scand Suppl 88
    [Google Scholar]
  10. Herriott R. M. 1951; Nucleic-acid-free T2 virus ‘ghosts’ with specific biological action. J. Bact 61:752
    [Google Scholar]
  11. Herriott R. M., Barlow J. L. 1952; Preparation, purification and properties of E. coli virus T2. J. gen. Physiol 36:17
    [Google Scholar]
  12. Hershey A. D., Chase M. 1952; Independent functions of viral protein and nucleic acid in growth of bacteriophage. J. gen. Physiol 36:39
    [Google Scholar]
  13. Hershey A. D., Kalmanson G., Bronfenbrenner J. 1943; Quantitative methods in the study of phage-antiphage reaction. J. Immunol 46:267
    [Google Scholar]
  14. Kellenberger G., Kellenberger E. 1952; La lysogénie d’une souche Bacillus cereus. Mise en évidence par le microscope électronique. Schweiz. Z. allg. Path 15:225
    [Google Scholar]
  15. Martin A. J. P., Porter R. R. 1951; The chromatographic fractionation of ribonuclease. Biochem. J 49:215
    [Google Scholar]
  16. Mickle H. 1948; Tissue disintegrator. J. R. micr. Soc 68:10
    [Google Scholar]
  17. Northrop J. H. 1938; Concentration and purification of bacteriophage. J. gen. Physiol 21:335
    [Google Scholar]
  18. Sevag M. G. 1934; Eine neue physikalische Enteiweissungsmethode zur Darstellung biologisch wirksamer Substanzen. Biochem. Z 273:419
    [Google Scholar]
  19. Smiles J., Welch F. V., Elford W. J. 1948; The influence of antibacterial substances on the interaction of bacteria and bacteriophages. II. Optical studies of the penicillin effect. J. gen. Microbiol 2:220
    [Google Scholar]
  20. Wahl R., Blum-Emerique L. 1949; Purification et concentration du bactériophage. II. Précipitation par l’ethanol et redissolution des phages-essais divers. Ann. Inst.Pasteur 76:103
    [Google Scholar]
  21. Wahl R., Lewi S. 1939; Étude de l’adsorption du bactériophage (du B. subtilis) sur l’hydrate d’alumine. C.R. Soc. Biol., Paris 131:591
    [Google Scholar]
  22. Wahl R., Terrade A., Monceaux G. 1950; Préparation des lysats bacterio- phagiques de titre élevé. Purification et concentration des phages par deux adsorptions successives sur phosphate de calcium. Ann. Inst. Pasteur 79:429
    [Google Scholar]
  23. Williams R. C., Backus R. C. 1949; Macromolecular weights determined by direct particle counting. I. The weight of the bushy stunt virus particle. J. Amer. chem. Soc 71:4052
    [Google Scholar]
  24. Wyckoff R. W. G. 1938; An ultracentrifugal analysis of concentrated staphylococcus bacteriophage preparations. J. gen. Physiol 21:367
    [Google Scholar]
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