1887

Abstract

Antigens specific for were investigated by double immunodiffusion in agar-gel. Antigenic materials were extracted from whole bacteria and some walls with cold trichloroacetic acid. Antisera were prepared by intravenous injection into rabbits of suspensions of whole organisms in solutions of bovine serum albumin, which had been heated and then washed. Four specific antigens were found as precipitinogens and denoted as antigens 11, 12, 13 and 14. Of 43 strains of studied, 33 strains possessed antigen 11, six strains antigen 12, two strains antigen 13 and two strains antigen 14. Sugar compositions of wall preparations were analysed in an attempt to characterize the determinants of antigens 11 and 12. The walls contained glucose, galactose, hexosamine and sometimes glycerol, but no rhamnose was found. It was considered that α-glucopyranose was the major component of the determinant of antigen 11 since trehalose and maltose significantly inhibited the reaction between antibody 11 and its antigen; the determinant of antigen 12 was not clarified.

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/content/journal/micro/10.1099/00221287-103-2-337
1977-12-01
2024-05-02
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References

  1. Bratthall D. 1970; Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontologisk revy 21:143–152
    [Google Scholar]
  2. Coyette J., Ghuysen J. M. 1970; Structure of the walls of Lactobacillus acidophilus strain 63 AM Gasser. Biochemistry 9:2935–2943
    [Google Scholar]
  3. Doyle R. J., Chatterjee A. N., Streeps U. N., Young F. E. 1975; Soluble macromolecular complexes involving bacterial teichoic acid. Journal of Bacteriology 124:341–347
    [Google Scholar]
  4. Draper L. R., Hirata A. A. 1968; Antibody responses in rabbits to soluble and particulate forms of bovine serum albumin. Immunology 15:23–30
    [Google Scholar]
  5. Efthymiou C., Hansen P. 1962; An antigenic analysis of Lactobacillus acidophilus. Journal of Infectious Diseases 110:258–267
    [Google Scholar]
  6. Fukazawa T., Shinoda T., Nakase T. 1973; Antibody response to Cryptococcus neoformans. 30th Scientific Meeting of the Japanese Bacteriological Society Kanto Branch. In Japanese.
    [Google Scholar]
  7. Gasser F. 1970; Electrophoretic characterization of lactic dehydrogenases in the genus Lactobacillus. Journal of General Microbiology 62:223–239
    [Google Scholar]
  8. Gilliand S. E., Speck M. L., Morgan C. G. 1975; Detection of Lactobacillus acidophilus in faeces of humans, pigs and chickens. Applied Microbiology 30:541–545
    [Google Scholar]
  9. Larm O., Lindberg B., Svensson S. 1971; Studies on the length of the side chain of the dextran elaborated by Leuconostoc mesenteroidesNRRL b-512. Carbohydrate Research 20:39–48
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin-phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  11. De Man J. C., Rogosa M., Sharpe M. E. 1960; A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology 23:130–135
    [Google Scholar]
  12. Mitsuoka T. 1969; Vergleichende Untersuchungen über die Lactobazillen aus den Faeces von Menschen, Schweinen und Hühnern. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrank-heiten und Hygiene Abteilung I, Originale 210:32–51
    [Google Scholar]
  13. Rogosa M., Sharpe M. E. 1959; An approach to the classification of the lactobacilli. Journal of Applied Microbiology 22:329–340
    [Google Scholar]
  14. Sharpe M. E. 1955; A serological classification of lactobacilli. Journal of General Microbiology 12:107–122
    [Google Scholar]
  15. Sharpe M. E. 1970; Cell wall and cell membrane antigens used in the classification of lactobacilli. International Journal of Systematic Bacteriology 20:509–518
    [Google Scholar]
  16. Sharpe M. E., Brock J. H., Knox K. W., Wicken A. J. 1973; Glycerol teichoic acid as a common antigenic factor in lactobacilli and some other Gram-positive organisms. Journal of General Microbiology 74:119–126
    [Google Scholar]
  17. Shimohashi H. 1975; Antigenic analyses of Lactobacillus fermenti. Japanese Journal of Microbiology 19:133–140
    [Google Scholar]
  18. Shimohashi H., Kamiyama K., Arai H. 1973; Studies on group antigens of Lactobacillus casei. Japanese Journal of Microbiology 17:205–210
    [Google Scholar]
  19. Shimohashi H., Kodaira S., Suegara N. 1976; Grouping antigens of four Lactobacillus species and their characteristics. Japanese Journal of Microbiology 20:405–413
    [Google Scholar]
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