1887

Abstract

Exopolysaccharide formation by 264 in a single-stage continuous culture was maximal under nitrogen limitation with excess carbohydrate substrate at 30±1 °C and pH 7·0±0·1 Polysaccharide production was not enhanced by phosphate limitation but was dependent on the dilution rate. Steady states were maintained for up to 500 h without deterioration of the culture or the development of mutant strains. The efficiency of conversion of the glucose substrate utilized into exopolysaccharide by the chemostat cultures was as high as 73%.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-104-1-47
1978-01-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/104/1/mic-104-1-47.html?itemId=/content/journal/micro/10.1099/00221287-104-1-47&mimeType=html&fmt=ahah

References

  1. Cooper C. M., Fernstrom G. A., Miller S. A. 1944; Performance of agitated gas-liquid contactors. Industrial and Engineering Chemistry 36:504–509
    [Google Scholar]
  2. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for the determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  3. Dudman W. F. 1964; Growth and extracellular polysaccharide production by Rhizobium meliloti in defined medium. Journal of Bacteriology 88:640–645
    [Google Scholar]
  4. Duguid J. P., Wilkinson J. F. 1953; The influence of cultural conditions on polysaccharide production by Aerobacter aerogenes. Journal of General Microbiology 9:174–189
    [Google Scholar]
  5. Eagon R. G. 1956; Studies on polysaccharide formation by Pseudomonas fluorescens. Canadian Journal of Microbiology 2:673–676
    [Google Scholar]
  6. Evans C. G. T., Herbert D., Tempest D. W. 1970; The continuous cultivation of microorganisms. II. Construction of a chemostat. Methods in Microbiology 2:277–328
    [Google Scholar]
  7. Evans L. R., Linker A. 1973; Production and characterisation of the slime of Pseudomonas aeruginosa. Journal of Bacteriology 116:915–924
    [Google Scholar]
  8. Fiske C. H., Subbarow Y. 1925; The colorimetric determination of phosphorus. Journal of Biological Chemistry 66:375–400
    [Google Scholar]
  9. Goto S., Enomoto S., Takahashi Y., Motomatsu R. 1971; Slime production by Pseudomonas aeruginosa. I. Conditions for slime production by the cellophane plate method. Japanese Journal of Microbiology 15:317–324
    [Google Scholar]
  10. Goto S., Murakawa T., Kuwahara S. 1973; Slime production by Pseudomonas aeroginosa. II. A new synthetic medium and cultural conditions for slime production by Pseudomonas aeruginosa. Japanese Journal of Microbiology 17:45–51
    [Google Scholar]
  11. Harada T., Yoshimura T., Hidaka H., Koreeda A. 1965; Production of a new acidic polysaccharide, succinoglucan, by Alcaligenes faecalis var.myxogenes. Agricultural and Biological Chemistry 29:757–762
    [Google Scholar]
  12. Lilly V. G., Wilson H. A., Leach J. G. 1958; Bacterial polysaccharides. II. Laboratory scale production of polysaccharide by species of Xanthomonas. Applied Microbiology 6:105–108
    [Google Scholar]
  13. Lindblom G. P., Patton J. T. 1967 U.S. Patent No. 3:328–262
    [Google Scholar]
  14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biologica Chemistry 193:265–275
    [Google Scholar]
  15. Moraine R. A., Rogovin P. 1966; Kinetics of polysaccharide B-1459 fermentation. Biotechnology and Bioengineering 8:511–524
    [Google Scholar]
  16. Moraine R. A., Rogovin P. 1971; Xanthan biopolymer production at increased concentration by pH control. Biotechnology and Bioengineering 13:381–391
    [Google Scholar]
  17. Moraine R. A., Rogovin P. 1973; Kinetics of the xanthan fermentation. Biotechnology and Bioengineering 15:225–237
    [Google Scholar]
  18. Norval M., Sutherland I. W. 1969; A group of Klebsiella mutants showing temperature- dependent polysaccharide synthesis. Journal of General Microbiology 57:369–377
    [Google Scholar]
  19. Parsons A. B., Dugan P. R. 1971; Production of extracellular polysaccharide matrix by Zoogloea ramigera. Applied Microbiology 21:657–661
    [Google Scholar]
  20. Pirt S. J. 1972; Prospects and problems in continuous flow culture of microorganisms. Journal of Applied Chemistry and Biotechnology 22:55–64
    [Google Scholar]
  21. Rogovin S. P. 1969 U.S. Patent No. 3:485–719
    [Google Scholar]
  22. Rogovin S. P., Anderson R. F., Cadmus R. F. 1961; Production of polysaccharide with Xanthomonas campestris. Biotechnology and Bioengineering 3:51–63
    [Google Scholar]
  23. Sandeord P. A., Pittsley J. E., Knutson C. A., Watson P. R., Cadmus M. C., Jeanes A. 1977; Variation in Xanthomonas campestris nrrl B.1459 : characterisation of xanthan products of differing pyruvic acid content. In Extracellular Microbial Polysaccharides A.C.S. Symposium Series 45 pp. 192–210 Sandford P.A, Laskin A. Edited by Washington: American Chemical Society.;
    [Google Scholar]
  24. Silman R. W., Rogovin S. P. 1970; Continuous fermentation to produce xanthan biopolymer: laboratory investigation. Biotechnology and Bioengineering 7:75–83
    [Google Scholar]
  25. Silman R. W., Rogovin S. P. 1972; Continuous fermentation to produce xanthan biopolymer: effect of dilution rate. Biotechnology and Bioengineering 14:23–31
    [Google Scholar]
  26. Solorzano L. 1969; Determination of ammonia in natural waters by the phenol-hypochlorite method. Limnology and Oceanography 14:799–801
    [Google Scholar]
  27. Tanzer J. M., Wood W. I., Krichevsky M. I. 1969; Linear growth kinetics of plaque-forming streptococci in the presence of sucrose. Journal of General Microbiology 58:125–133
    [Google Scholar]
  28. Unz R. F., Farrah S. R. 1976; Exopolymer production and flocculation by Zoogloea mp6. Applied and Environmental Microbiology 31:623–626
    [Google Scholar]
  29. Wilkinson J. F., Duguid J. P., Edmunds P. N. 1954; The distribution of polysaccharide production in Aerobacter and Escherichia strains and its relation to antigenic character. Journal of General Microbiology 11:59–72
    [Google Scholar]
  30. Williams A. R. 1969; An electromagnetic modification of the Zimm-Crothers viscometer. Journal of Scientific Instruments 2:279–281
    [Google Scholar]
  31. Williams A. G. 1974 Extracellular polysaccharide production by a Gram-negative bacterial isolate. Ph.D. thesis University of Wales:
    [Google Scholar]
  32. Williams A. G., Wimpenny J. W. T. 1976; Exopolysaccharide production by Pseudomonas pbi in batch and continuous culture: effect of growth conditions. Journal of Applied Chemistry and Biotechnology 26:326–327
    [Google Scholar]
  33. Williams A. G., Wimpenny J. W. T. 1977; Exopolysaccharide production by Pseudomonas ncib11264 grown in batch culture. Journal of General Microbiology 102:13–21
    [Google Scholar]
  34. Williams A. G., Wimpenny J. W. T., Lawson C. J. 1973; The production of an extracellular polysaccharide by a Pseudomonas-type microorganism. Journal of General Microbiology 77:xiii
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-104-1-47
Loading
/content/journal/micro/10.1099/00221287-104-1-47
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error