1887

Abstract

Summary: Erythromycin-resistant mutants mapping to the Mendelian -M2 locus of were cold-sensitive for growth at 15 °C. The phenotypes erythromycin-resistance and cold-sensitivity could not be separated in crosses.

Mutants at the -M2 locus were previously found to have chloroplast ribosomes with altered [C]erythromycin binding capacity ( Mets & Bogorad, 1971 ). When grown at 25 °C, haploid strains containing -M2 alleles alone or in combination with other loci had fewer chloroplast ribosomes than the wild type. A further reduction occurred when -M2 strains were shifted to 15 °C. Diploids containing both -M2 and its wild-type allele contained normal amounts of chloroplast ribosomes at 25 and 15°C, and were cold-resistant and antibiotic-sensitive.

The inability of chloroplast ribosome-deficient -M2 strains to grow at 15 °C in the presence of a reduced carbon source indicates that chloroplast protein synthesis is essential for cellular growth even when photosynthesis is not essential.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-105-2-253
1978-04-01
2024-05-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/105/2/mic-105-2-253.html?itemId=/content/journal/micro/10.1099/00221287-105-2-253&mimeType=html&fmt=ahah

References

  1. Boynton J.E., Gillham N.W., Chabot J.F. 1974; Chloroplast ribosome deficient mutants in the green alga Chlamydomonas reinhardi and the question of chloroplast ribosome function. Journal of Cell Science 10:267–305
    [Google Scholar]
  2. Cattolico R.A., Jones R.F. 1972; Isolation of stable ribosomal RNA from whole cells of Chlamydomonas reinhardii.. Biochimica et bio-physica acta 269:259–264
    [Google Scholar]
  3. Cattolico R.A., Senner J.W., Jones R.F. 1973; Changes in cytoplasmic and chloroplast ribosomal RNA during the cell cycle of Chlamydomonas reinhardi.. Archives of Biochemistry and and Biophysics 156:58–65
    [Google Scholar]
  4. Davidson J.N. 1976 Genes affecting erythromycin resistance in ery-Ml >mutants of Chlamydomonas reinhardi. Ph.D. thesis Harvard University, U.S.A.:
    [Google Scholar]
  5. Davidson J.N., Bogorad L. 1977; suppression of erythromycin resistance in ery- Ml mutants of Chlamydomonas reinhardi.. Molecular and General Genetics in the Press
    [Google Scholar]
  6. Davies J., Nomura M. 1972; The genetics of bacterial ribosomes. Annual Review of Genetics 6:203–234
    [Google Scholar]
  7. Ebersold W.T. 1967; Chlamydomonas reinhardi: heterozygous diploid strains. Science 157:447–449
    [Google Scholar]
  8. Feunteun J., Monier R., Vola C., Rosset R. 1974; Ribosomal assembly and defective mutants of Escherichia coli.. Nucleic Acids Research 1:141–148
    [Google Scholar]
  9. Flaks J.G., Leboy P.S., Birge E.H., Kurland C.G. 1966; Mutation and genetics concerned with the ribosome. Cold Spring Harbor Symposia on Quantitative Biology 31:623–631
    [Google Scholar]
  10. Gillham N.W. 1963; Transmission and seg-regation of a non-chromosomal factor controlling streptomycin resistance in diploid Chlamydomonas.. Nature; London: 200294
    [Google Scholar]
  11. Goodenough U.W., Togasaki R.K., Paszewski A., Levine R.P. 1971; Inhibition of chloroplast ribosome formation by gene mutation in Chlamydomonas reinhardi.. In Autonomy and Bio-genesis of Mitochondria and Chloroplasts pp. 224–234 Boardman N.K., Linnane A.W., Smillie R.M. Edited by Amsterdam: North-Holland;
    [Google Scholar]
  12. Guerola N., Cerda-Olmeda E. 1975; Dis-tribution of mutations induced by ethyl meth-anesulfonate and ultraviolet radiation in the Escherichia coli chromosome. Mutation Research 29:145–147
    [Google Scholar]
  13. Guerola N., Ingraham J.L., Cerda-olmeda E. 1971; Induction of closely linked multiple mutations by nitrosoguanidine. Nature New Biology 230:122–125
    [Google Scholar]
  14. Hanson M.R., Bogorad L. 1977a; Complementation analysis at the ery- Ml locus in Chlamy-domonas reinhardi.. Molecular and General Genetics 153:271–277
    [Google Scholar]
  15. Hanson M.R., Bogorad L. 1977b; . Effects of erythromycin on membrane-bound chloroplast ribosomes from wild-type Chlamydomonas reinhardi and erythromycin-resistant mutants. Biochimica et biophysica acta 479:279–289
    [Google Scholar]
  16. Harris E.H., Boynton J.E., Gillham N.W. 1974; Chloroplast ribosome biogenesis in Chlamydomonas. Selection and characterization of mutants blocked in ribosome formation. Journal of Cell Biology 63:160–179
    [Google Scholar]
  17. Levine R.P., Ebersold W.T. 1960; The genetics and cytology of Chlamydomonas.. Annual Review of Microbiology 14:197–216
    [Google Scholar]
  18. Mets L.J., Bogorad L. 1971; Mendelian and uniparental alterations in erythromycin binding by plastid ribosomes. Science 174:707–709
    [Google Scholar]
  19. Mets L.J., Bogorad L. 1972; Altered chloroplast ribosomal proteins associated with erythromycin-resistant mutants in two genetic systems of Chlamydomonas reinhardi.. Proceedings of the National Academy of Sciences of the United States of America 69:3779–3783
    [Google Scholar]
  20. Nashimoto H., Held W., Kaltschmidt E., Nomura M. 1971; Structure and function of bacterial ribosomes. XII. Accumulation of 21S particles by some cold-sensitive mutants of Escherichia coli.. Journal of Molecular Biology 62:121–138
    [Google Scholar]
  21. Pardo D., Rosset R. 1974; Genetic studies of erythromycin resistant mutants of Escherichia coli.. Molecular and General Genetics 135:257–268
    [Google Scholar]
  22. Simon R.D. 1974; The use of an ultrasonic bath to disrupt cells suspended in volumes of less than 100 microliters. Analytical Biochemistry 60:51–58
    [Google Scholar]
  23. Sueoka N. 1960; Mitotic replication of DNA in Chlamydomonas reinhardi.. Proceedings of the National Academy of Sciences of the United States of America 46:83–91
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-105-2-253
Loading
/content/journal/micro/10.1099/00221287-105-2-253
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error