1887

Abstract

SUMMARY: When was harvested from citrate + mineral salt media in which the concentration of carbon source limited the crop, citridesmolase activity of cells was high but was absent when growth ceased with citrate in excess. These differences were abolished by disintegration of the cells. The ability of to dissimilate citrate was measurable only after growth in a medium containing peptone, citrate and glucose: when the latter was consumed activity fell and was revived by further glucose additions. Differences in activity of such cells were not abolished by their disintegration. Cells lost their activity when incubated for 30 min. with phosphate buffer or with the citrate + mineral salt medium that supports growth of grown at the expense of glucose in a mineral salt medium + citrate, did not utilize citrate for growth during the first day and no citridesmolase activity could be detected, but when subsequently tested in a medium which permitted development of activity, higher activities were reached by cells that had grown in presence of citrate. grown in peptone + citrate + glucose medium possessed little oxaloacetate decarboxylase; by the action of disintegrated cells, citrate gave rise to equimolar quantities of keto-acid, chiefly oxaloacetic acid.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-11-2-218
1954-10-01
2024-05-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/11/2/mic-11-2-218.html?itemId=/content/journal/micro/10.1099/00221287-11-2-218&mimeType=html&fmt=ahah

References

  1. Barrett J., Larson A., Kallio R. 1953; The nature of the adaptive lag of Pseudomonas fluorescens toward citrate. J. Bact 65:187
    [Google Scholar]
  2. Billen D., Lichstein H. C. 1951; Nutritional requirements for the production of formic hydrogenlyase, formic dehydrogenase, and hydrogenase in Escherichia coli. J. Bact 61:515
    [Google Scholar]
  3. Brewer C. R., Werkman C. H. 1939; The anaerobic dissimilation of citric acid by Aerobacter indologenes. Enzymologia 6:273
    [Google Scholar]
  4. Dagley S., Dawes E. A. 1953a; Citric acid metabolism of Aerobacter aerogenes. J. Bact 66:259
    [Google Scholar]
  5. Dagley S., Dawes E. A. 1953b; Dissimilation of citric acid by bacterial extracts. Nature; Lond: 172345
    [Google Scholar]
  6. Dagley S., Dawes E. A., Morrison G. A. 1950; Inhibition of growth of Aerobacter aerogenes: the mode of action of phenols, alcohols, acetone and ethyl acetate. J. Bact 60:369
    [Google Scholar]
  7. Dagley S., Fewster M. E., Happold F. C. 1952; The bacterial oxidation of phenylacetic acid. J. Bact 63:327
    [Google Scholar]
  8. Friedemann T. E., Haugen G. E. 1943; Pyruvic acid. II. The determination of keto-acids in blood and urine. J. biol. Chem 147:415
    [Google Scholar]
  9. Gillespie D. C., Gunsalus I. C. 1953; An adaptive citric acid desmolase in Streptococcus faecalis. Bact. Proc p. 80
    [Google Scholar]
  10. Grunberg-Manago M., Gunsalus I. C. 1953; Aerobic and anaerobic citric acid metabolism of Escherichia coli. Bact. Proc p. 73
    [Google Scholar]
  11. Hughes D. E. 1951; A press for disrupting bacteria and other micro-organisms. Brit. J. exp. Path 32:97
    [Google Scholar]
  12. Isherwood F. A., Cruickshank D. H. 1954; Chromatographic separation and analysis of mixtures of pyruvic, oxalacetic and alpha-ketoglutaric acids. Nature; Lond: 173121
    [Google Scholar]
  13. Kogut M., Podoski E. P. 1953; Oxidative pathways in a fluorescent Pseudomonas. Biochem. J 55:800
    [Google Scholar]
  14. Koser S. A. 1924; Correlation of citrate utilization by members of the colonaerogenes group, with other differential characteristics and with habitat. J. Bact 9:59
    [Google Scholar]
  15. Lara F. J. S., Stokes J. L. 1952; Oxidation of citrate by Escherichia coli. J. Bact 63:415
    [Google Scholar]
  16. Lascelles J. 1948; Studies on formic hydrogenlyase in Escherichia coli. Biochem. J 43:vi
    [Google Scholar]
  17. Lominski I., Conway N. S., Harper E. M., Rennie J. B. 1947; Utilization of citric acid by some so-called citrate-non-utilizing bacteria. Nature; Lond: 160573
    [Google Scholar]
  18. Pinsky M. J., Stokes J. L. 1952; Requirements for formic hydrogenlyase adaptation in nonproliferating suspensions of Escherichia coli. J. Bact 64:151
    [Google Scholar]
  19. Somogyi M. 1937; A reagent for the copper-iodometric determination of very small amounts of sugar. J. biol. Chem 117:771
    [Google Scholar]
  20. Spiegelman S., Halvorson H. O. 1953; The nature of the precursor in the induced synthesis of enzymes. Symp. Soc. gen. Microbiol 3:98
    [Google Scholar]
  21. Stern J. R., Ochoa S. 1951; Enzymatic synthesis of citric acid I. Synthesis with soluble enzymes. J. biol. Chern 191:161
    [Google Scholar]
  22. Taylor E. S. 1947; The assimilation of amino-acids by bacteria. 3. Concentration of free amino-acids in the internal environment of various bacteria and yeasts. J. gen. Microbiol 1:86
    [Google Scholar]
  23. Umbreit W. W., Burris R. H., Stauffer J. F. 1945 talic>Manometric Techniques and related Methods for the Study of Tissue Metabolism Minneapolis, Minn: Burgess;
    [Google Scholar]
  24. Varley H. 1954 Practical Clinical Biochemistry p. 141 London: Heinemann;
    [Google Scholar]
  25. Vaughn R. H., Osborne J. T., Wedding G. T., Tabachnick J., Beisel C. G., Braxton T. 1950; The utilization of citrate by Escherichia coli. J. Bact 60:119
    [Google Scholar]
  26. Virtanen A. I., Miettinen J. K., Kunttu H. 1953; Alpha-ketoacids in green plants. Acta chem. Scand 7:38
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-11-2-218
Loading
/content/journal/micro/10.1099/00221287-11-2-218
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error