1887

Abstract

SUMMARY: NCIB 7581, when growing exponentially in a simple chemically defined medium (without 2,6-diaminopimelic acid), contained only 4 m-diaminopimelate in the free amino acid pool; this diaminopimelate was 84% (w/w) -isomer and 16% -isomer. Growing organisms could take up any of the three isomers of diaminopimelate from the medium, though the -isomer was taken up at only half the rate of the other two isomers (- and -). When lysine was also present in the medium, most (70%, w/w) of the diaminopimelate that was taken up entered peptidoglycan; only about 5% of the total uptake was into the free amino acid pool. When -diamino[C]pimelate was supplied in the medium, 86% of the diamino[C]pimelate incorporated into peptidoglycan was present as the -isomer and 14% was the -isomer.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-110-2-401
1979-02-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/110/2/mic-110-2-401.html?itemId=/content/journal/micro/10.1099/00221287-110-2-401&mimeType=html&fmt=ahah

References

  1. Day A., White P. J. 1977; Enzymic assays for isomers of 2,6-diaminopimelic acid in walls of Bacillus cereus and Bacillus megaterium. Biochemical Journal 161:677–685
    [Google Scholar]
  2. Leive L., Davis B. D. 1965; The transport of diaminopimelate and cystine in Escherichia coli. Journal of Biological Chemistry 240:4362–4369
    [Google Scholar]
  3. Meadow P. M., Work E. 1958; Bacterial transamination of the stereoisomers of diaminopimelic acid and lysine. Biochimica et biophysica acta 28:596–599
    [Google Scholar]
  4. Meadow P. M., Hoare D. S., Work E. 1957; Interrelationships between lysine and αε-diaminopimelic acid and their derivatives and analogues in mutants of Escherichia coli. Biochemical Journal 66:270–282
    [Google Scholar]
  5. Park J. T., Hancock R. 1960; A fractionation procedure for studies of the synthesis of cell-wall mucopeptide and of other polymers in cells of Staphylococcus aureus. Journal of General Microbiology 22:249–258
    [Google Scholar]
  6. Perkins H. R. 1965; 2,6-Diamino-3-hydroxypimelic acid in microbial cell wall mucopeptide. Nature, London 208:872–873
    [Google Scholar]
  7. Pitel D. W., Gilvarg C. 1970; Mucopeptide metabolism during growth and sporulation in Bacillus megaterium. Journal of Biological Chemistry 245:6711–6717
    [Google Scholar]
  8. Rhuland L. E., Work E., Denman R. F., Hoare D. S. 1955; The behaviour of isomers of α,ε-diaminopimelic acid on paper chromatograms. Journal of the American Chemical Society 77:4844–4846
    [Google Scholar]
  9. Rosner A. 1975; Control of lysine biosynthesis in Bacillus subtilis: inhibition of diaminopimelate decarboxylase by lysine. Journal of Bacteriology 121:20–28
    [Google Scholar]
  10. Saleh F., White P. J. 1976; Use of auxotrophic mutants to isolate ll- or dd-isomers of 2,6- diaminopimelic acid. Journal of General Microbiology 96:253–261
    [Google Scholar]
  11. Tempest D. W., Meers J. L., Brown C. M. 1970; Influence of environment on the content and composition of microbial free amino acid pools. Journal of General Microbiology 64:171–185
    [Google Scholar]
  12. White P. J. 1971; Diaminopimelate decarboxylase (Escherichia coli). Methods in Enzymology 17B:140–145
    [Google Scholar]
  13. White P. J. 1972; The nutrition of Bacillus megaterium and Bacillus cereus. Journal of General Microbiology 71:505–514
    [Google Scholar]
  14. White P. J., Kelly B., Suffling A., Work E. 1964; Variation of activity of bacterial diaminopimelate decarboxylase under different conditions of growth. Biochemical Journal 91:600–610
    [Google Scholar]
  15. White P. J., Lejeune B., Work E. 1969; Assay and properties of diaminopimelate epimerase from Bacillus megaterium. Biochemical Journal 113:589–601
    [Google Scholar]
  16. Wickus G. G., Strominger J. L. 1972; Penicillin-sensitive transpeptidation during peptidoglycan biosynthesis in cell-free preparations from Bacillus megaterium. 1. Incorporation of free diaminopimelic acid into peptidoglycan. Journal of Biological Chemistry 247:5297–5306
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-110-2-401
Loading
/content/journal/micro/10.1099/00221287-110-2-401
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error