1887

Microbiology Society logo

Volume 111, Issue 2

Antigenic Cross-reactivity of Major Outer Membrane Proteins in Enterobacteriaceae Species Free

Abstract

The protein constituents in the outer membrane (OM) of several serotypes of and some other Enterobacteriaceae cross-reacted antigenically. Solubilized OM preparations of these bacteria were applied in interfacial precipitin tests to antisera elicited in rabbits against whole bacterial cells, absorbed with their appropriate lipopolysaccharide before testing. The resulting immunecomplexes were analysed on polyacrylamide gels. Protein profiles of the immunoprecipitates showed a considerable antigenic cross-reactivity of outer membrane proteins between most serotypes. Cross-reactivity, though substantially lower, was also found with OM from three other Enterobacteriaceae species, but was not detectable with OM. When OM preparations were solubilized at room temperature, the peptidoglycan-bound proteins in the molecular weight range 37000 to 41000 predominated in the protein profiles of the immunecomplexes. In profiles of immunecomplexes obtained with boiled OM preparations, a heat-modifiable protein (mol. wt 33000) predominated. The major OM proteins of the Gram-negative bacterium may therefore play a role as common surface antigens of the family of Entero-bacteriaceae.

  • Received:
  • Published Online:
© Society for General Microbiology 1979
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-111-2-293
1979-04-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/111/2/mic-111-2-293.html?itemId=/content/journal/micro/10.1099/00221287-111-2-293&mimeType=html&fmt=ahah

References

  1. AMES G. F. 1974; Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions. Journal of Biological Chemistry 249:634–644
     [Google Scholar]
  2. BRAUN V. 1975; Covalent lipoprotein from the outer membrane of Escherichia coli.. Biochimicaet biophysica acta 415:335–377
     [Google Scholar]
  3. BRAUN V., BOSCH V.,, KLUMPP E. R., NEFF, I., MAYER H., SCHLECHT S. 1976; Antigenic determinants of murein lipoprotein and its exposure the surface of Enterobacteriaceae.. European Journal of Biochemistry 62:555–566
     [Google Scholar]
  4. CHEDID L., PARANT M.,, PARANT F., BOYER F. 1968; A proposed mechanism for natural immunity to enterobacterial pathogens.. Journal of Immunology 100:292–301
     [Google Scholar]
  5. COWAN S. T., STEEL K. J. 1965; Manual for the Identification of Medical Bacteria.. Cambridge :: Cambridge University Press.;
     [Google Scholar]
  6. DANKERT J., HOFSTRA H. 1978; Antibodies against outer membrane proteins in rabbit antisera prepared against Escherichia coli 026 K60.. Journal of General Microbiology 104:311–320
     [Google Scholar]
  7. ELLWOOD D. C. 1970; The distribution of 2-keto-3-deoxy-octonic acid in bacterial walls. Journal of General Microbiology 60:373–380
     [Google Scholar]
  8. GALANOS C.,, LÜUDERITZ O., WESTPHAL O. 1971; Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides.. European Journal of Biochemistry 24:116–122
     [Google Scholar]
  9. GARTEN W.,, HINDENNACH I., HENNING U. 1975; The major proteins of the Escherichia coli outer cell envelope membrane. Characterization of proteins I1 and 111, comparison of all proteins.. European Journul of Biochemistry 59:215–221
     [Google Scholar]
  10. HENNING U.,, HÖOHN B., SONNTAG I. 1973; Cell envelope and shape of Escherichia Loli K12. The ghost membrane. European Journal of Biochemistry 39:27–36
     [Google Scholar]
  11. HINDENNACH I.,, HENNING U. 1975; The major proteins of the Escherichia coli outer cell envelope membrane. Preparative isolation of all major membrane proteins.. European Journal of Biochemistry 59:207–213
     [Google Scholar]
  12. KALTSER B., AHLSTEDT S. 1977; Protective capacity of antibodies against Escherichia coli 0 and K antigens.. Infection and Immunity 17:286–289
     [Google Scholar]
  13. KUNIN, C.M., BEARD M. V., HALMAGYI E. 1962; Evidence for a common hapten associated with endotoxin fractions of Escherichia coli and other En terobacteriaceae. Proceedings of the Society for Experimental Biology and Medicine 111:160–166
     [Google Scholar]
  14. KWAPINSKI J. B. G. The precipitation test.. In Kwapinski J. B. G. Methodology of’ Immunochemicul and Immunological Research pp. New York: John Wiley.;317–332 1972
     [Google Scholar]
  15. LAEMMLI U.K. 1970; Cleavage of the structural proteins during the assembly of the head of bacteriophage T4.. Nature, London: 227:680–685
     [Google Scholar]
  16. LOWRY O.H., ROSEBROUGH N.J.,, RANDALL R. J. 1951; Protein measurement with the Folin phenol reagent.. Journul of Biological Chemistry 193:265–275
     [Google Scholar]
  17. LUGTENBERG B.,, MEYERS J.,, PETERS R., VAN DERHOEK P., VAN ALPHEN L. 1975; Electrophoretic resolution of the ‘Major outer membrane protein’ of Escherichia coli K12 into four bands.. FEBS Letters 58:254–258
     [Google Scholar]
  18. LUGTENBERG B.,, BRONSTEIN H.,, VAN SELM N., PETERS R. 1977; Peptidoglycan-associatedo uter membrane proteins in Gram-negative bacteria.. Biochimica et biophysica acta 465:571–578
     [Google Scholar]
  19. MANNING P. A., REEVES P. 1977; Outer membrane protein 3B of Escherichia coli K12: effects of growth temperature on the amount of the protein and further characterization on acrylamide gels.. FEMS Microbiology Letters 1:275–578
     [Google Scholar]
  20. MCCABE W. R., GREELY A. 1973; Common Enterobacterial Antigen. 11. Effect of immunization on challenge with heterologous bacilli.. Infection and Immunity 7:386–392
     [Google Scholar]
  21. MCCABE W. R., BRUINS S. C.,, CRAVEN D. E.,, JOHNS M. 1977; Cross-reactive antigens : their potential for immunization-induced immunity to Gram-negative bacteria. Journal of Infectious Diseases 136 suppl.,:S161–S166
     [Google Scholar]
  22. MULLAN N. A.,, NEWSOME P. M.,, CUNNINGTON P. G.,, JOHNS M., PALMER G. H., WILSON M. E. 1974; Protection against Gram-negative infections with antiserum to lipid A from Salmonella minnesota R595.. Infection and Immunity 10:1195–1201
     [Google Scholar]
  23. NETER E., KENNEDY E. A., JEWETT T. C. 1974; An tibody response to common enterobacterial antigen of children with pyogenic peritonitis.. Infection 1:12–16
     [Google Scholar]
  24. NG, A.K, CHEN C. H., CHANG C. M., NOWOTNY, A. 1976; Relationship of structure to function in bacterial endotoxins : serologically crossreactive components and their effect on protection of mice against some Gram-negative infections.. Journal of General Microbiology 94:107–116
     [Google Scholar]
  25. SCHNAITMAN C. A. 1970; Comparison of the envelope protein compositions of several Gramnegative bacteria. Journal of Bacteriology 104:1404–1405
     [Google Scholar]
  26. SCHNAITMAN C. A. 1971; Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100. Journal of Bacteriology 108:545–552
     [Google Scholar]
  27. TATE W. J., DOUGLAS H, BRAUDE A. I. 1966; Protection against lethality of Escherichia coli endotoxin with O antiserum. Annals of the New York Academy of Sciences 133:746–762
     [Google Scholar]
  28. VOGEL H. J., BONNER D. M. 1956; A convenient growth medium for Escherichia coli and some other microorganisms. Microbial Genetics Bidletin 13:43–44
     [Google Scholar]
  29. WESTPHAL. O., LUDERITZ O., BISTER F. 1952; Uber die Extraktion von Bakterien mit Phenol-Wasser. Zeitschrift fur Ncturforschung 7b:148–155
     [Google Scholar]
  30. YOUNG L. S., STEVENS P., INGRAM. J. 1975; Functional role of antibody against core glycolipid of Enterobacteriaceae. Journol of Clinical Investigation 56:850–861
     [Google Scholar]
  31. ZIEGLER E. J., DOUGLAS. H., SHERMAN J. E., DAVIS C. E., BRAUDE A. I. 1973; Treatment of E. coli and Klebsiella bacteremia in agranulocytic animals with antiserum to a UDP-gal epimerase-deficient mutant. Journal of Immunology 111:433–438
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-111-2-293
Loading
Antigenic Cross-reactivity of Major Outer Membrane Proteins in Enterobacteriaceae Species
Microbiology 111, 293 (1979); https://doi.org/10.1099/00221287-111-2-293
/content/journal/micro/10.1099/00221287-111-2-293
/content/journal/micro/10.1099/00221287-111-2-293
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error