1887

Abstract

Summary: strain Ingbritt, and its derivative B7 which had been passaged through monkeys, have been used to investigate how the synthesis of extracellular glucosyl- and fructosyltransferases is regulated. The most active enzyme from carbon-limited continuous cultures was a fructosyltransferase; enzymes catalysing the formation of water-insoluble glucans from sucrose were relatively inactive. Dextransucrase (EC 2.4.1.5), which catalyses soluble glucan synthesis, was most active in the supernatant fluid from cultures grown with excess glucose, fructose or sucrose, but full activity was detected only when the enzyme was incubated with both sucrose and dextran. Little dextransucrase activity was detected in carbon-limited cultures. It is concluded that glucosyl- and fructosyltransferases are constitutive enzymes in that they are synthesized at similar rates during growth with an excess of the substrate or of the products of the reactions which they catalyse. Although the Ingbritt strain was originally isolated from a carious lesion, it is now a poor source of glucosyltransferase activity. Glucosyltransferases were extremely active in cultures of a recent clinical isolate, strain 3209, and were apparently induced during growth with excess glucose.

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1979-09-01
2024-05-07
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References

  1. Baird J.K., Longyear V.M.C., Ellwood D.C. 1973; Water insoluble and soluble glucans produced by extracellular glycosyltransferases from Streptococcus mutans. . Microbios 8:143–150
    [Google Scholar]
  2. Beeley J.A., Black P.M. 1977; Glucosyltransferase production by Streptococcus sanguis804. Infection and Immunity 15:50–58
    [Google Scholar]
  3. Burckhardt J.J., Guggenheim B. 1976; Interactions of antisera, sera and oral fluid with glucosyltransferases. Infection and Immunity 13:1009–1022
    [Google Scholar]
  4. Carlsson J., Griffith C.J. 1974; Fermentation products and bacterial yields in glucose-limited and nitrogen-limited cultures of streptococci. Archives of Oral Biology 19:1105–1109
    [Google Scholar]
  5. Chassy B.M., Beall J.R., Bielawski R.M., Porter E.V., Donkersloot J.A. 1976; Occurrence and distribution of sucrose-metabolizing enzymes in oral streptococci. Infection and Immunity 14:408–415
    [Google Scholar]
  6. Chludzinski A.M., Germaine G.R., Schachtele C.F. 1974; Purification and properties of dextransucrase from Streptococcus mutans. Journal of Bacteriology 118:1–7
    [Google Scholar]
  7. Ciardi J.E., Beaman A.J., Wittenberger C.L. 1977; Purification, resolution and interaction of the glucosyltransferases of Streptococcus mutans6715. Infection and Immunity 18:237–246
    [Google Scholar]
  8. Cole J.A. 1977; A biochemical approach to the control of dental caries. Biochemical Society Transactions 5:1232–1239
    [Google Scholar]
  9. De Stoppelaar J.D., Konig K.G., Plasschaert A.J.M., van Der Hoeven J.S. 1971; Decreased cariogenicity of a mutant of Streptococcus mutans. Archives of Oral Biology 16:971–975
    [Google Scholar]
  10. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  11. Dubowski K.M. 1962; An o-toluidine method for body-fluid glucose determination. Clinical Chemistry 8:215–235
    [Google Scholar]
  12. Ellwood D.C., Hunter J.R. 1976; The mouth as a chemostat. In Continuous Cultures: Application and New Fields Proceedings of the 6th International Symposium on Continuous Culture of Micro-organisms pp. 270–282 Edited by Dean A. C. R., Ellwood D. C., Evans C. G. J., Melling J. Chichester: Ellis Horwood;
    [Google Scholar]
  13. Ellwood D.C., Hunter J.R., Longyear V.M.C. 1974; Growth of Streptococcus mutans in a chemostat. Archives of Oral Biology 19:659–664
    [Google Scholar]
  14. Ellwood D.C., Baird J.K., Hunter J.R., Longyear V.M.C. 1976; Variations in surface polymers of Streptococcus mutans. Journal of Dental Research 55 C42: C49;
    [Google Scholar]
  15. Freedman M.L., Tanzer J.M. 1974; Dissociation of plaque formation from glucan- induced agglutination in mutants of Streptococcus mutans. Infection and Immunity 10:189–196
    [Google Scholar]
  16. Fukui K., Fukui Y., Moriyama T. 1974; Acceleration of dextransucrase activity of Streptococcus mutans by secretory immunoglobulin A. Journal of Bacteriology 118:804–809
    [Google Scholar]
  17. Germaine G.R., Schachtele C.F. 1976; Streptococcus mutans dextransucrase mode of interaction with high-molecular-weight dextran and role in cellular aggregation. Infection and Immunity 13:365–372
    [Google Scholar]
  18. Germaine G.R., Chludzinski A.M., Schachtele C.F. 1974; Streptococcus mutans dextransucrase: requirement for primer dextran. Journal of Bacteriology 120:287–294
    [Google Scholar]
  19. Gibbons R.J., Fitzgerald R.J. 1969; Dextraninduced agglutination of Streptococcus mutans and its potential role in the formation of microbial dental plaques. Journal of Bacteriology 98:341–346
    [Google Scholar]
  20. Gibbons R.J., van Houte J. 1975; Bacterial adherence in oral microbial ecology. Annual Review of Microbiology 29:19–44
    [Google Scholar]
  21. Gibbons R.J., Berman K.S., Knoettner P., Kapsimalis B. 1966; Dental caries and alveolar bone loss in gnotobiotic rats infected with capsule forming streptococci of human origin. Archives of Oral Biology 11:549–560
    [Google Scholar]
  22. Guggenheim B., Regolati B., Muhlemann H.R. 1972; Caries and plaque inhibition by mutanase in rats. Caries Research 6:289–297
    [Google Scholar]
  23. Hamada S., Torii M. 1978; Effect of sucrose in culture media on the location of glucosyltrans-ferase of Streptococcus mutans and cell adherence to glass surfaces. Infection and Immunity 20:592–599
    [Google Scholar]
  24. Hojo S., Higuchi M., Araya S. 1976; Glucan inhibition of diffusion in plaque. Journal of Dental Research 55:169
    [Google Scholar]
  25. Janda W.M., Kuramitsu H.K. 1976; Regulation of extracellular glucosyltransferase production and the relationship between extracellular and cell-associated activities in Streptococcus mutans. Infection and Immunity 14:191–202
    [Google Scholar]
  26. Janda W.M., Kuramitsu H.K. 1978; Production of extracellular and cell-associated glucosyltransferase activity by Streptococcus mutans during growth on various carbon sources. Infection and Immunity 19:116–122
    [Google Scholar]
  27. Keene J.H., Shklair I.L., Mickel G.J., Wirthlin M.R. 1977; Distribution of Streptococcus mutans biotypes in five human populations. Journal of Dental Research 56:5–10
    [Google Scholar]
  28. Keevil C.W., Hough J.S., Cole J.A. 1979; Regulation of respiratory and fermentative modes of growth of Citrobacter freundii by oxygen, nitrate and glucose. Journal of General Microbiology 113:83–95
    [Google Scholar]
  29. Koga T., Inoue M. 1978; Cellular adherence, glucosyltransferase adsorption and glucan synthesis of Streptococcus mutans AHT mutants. Infection and Immunity 19:402–410
    [Google Scholar]
  30. Kulka R.G. 1956; Colorimetric estimation of ketopentoses and ketohexoses. Biochemical Journal 63:542–549
    [Google Scholar]
  31. Lennox E.S. 1955; Transduction of linked genetic characters of the host by bacteriophage PI. Virology 1:190–206
    [Google Scholar]
  32. McCabe M.M., Smith E.E. 1973; Origin of the cell-associated dextran sucrase of Streptococcus mutans. Infection and Immunity 1:829–838
    [Google Scholar]
  33. Montville T.J., Cooney C.L., Sinskey A.J. 1977; Distribution of dextransucrase in Streptococcus mutans and observations on the effect of soluble dextran on dextransucrase activities. Infection and Immunity 18:629–635
    [Google Scholar]
  34. Nalbandian J., Freedman M.L., Tanzer J.M., Lovelace J.M. 1974; Ultrastructure of mutants of Streptococcus mutans with reference to agglutination adhesion and extracellular polysaccharide. Infection and Immunity 10:1170–1179
    [Google Scholar]
  35. Robrish S.A., Reid W., Krichevsky M.I. 1972; Distribution of enzymes forming polysaccharide from sucrose and the composition of extracellular polysaccharide synthesised by Streptococcus mutans. Applied Microbiology 24:184–190
    [Google Scholar]
  36. Schachtele C.F., Harlander S.K., Germaine G.R. 1976; Streptococcus mutans dextransucrase: availability of disaggregated enzyme after growth in a chemically defined medium. Infection and Immunity 13:1522–1524
    [Google Scholar]
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