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Volume 116, Issue 1

A Cellophane Membrane Method for Screening Auxotrophic Mutants of Photochromogenic Mycobacteria Free

Abstract

A high-resolution screening procedure for the selection of mutants was developed based on the ability of some strains of to produce pigment in the light. Out of 23000 colonies investigated, 17 auxotrophic mutants were isolated. Specific growth factor requirements for the mutants and the proportion of revertants were determined.

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© Society for General Microbiology 1980
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/content/journal/micro/10.1099/00221287-116-1-253
1980-01-01
2024-05-17
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References

  1. Davis B. D. 1948; Isolation of biochemically deficient mutants of bacteria by penicillin. Journal of the American Chemical Society 70:4267–4269
     [Google Scholar]
  2. Holland K. T., Ratledge C. 1971; A procedure for selecting and isolating specific auxotrophic mutants of Mycobacterium smegmatis. Journal of General Microbiology 66:115–118
     [Google Scholar]
  3. Koníčková-Radochová M., Koníček J., Málek I. 1970; The study of mutagenesis in Mycobacterium phlei. Folia microbiclogica 15:88–102
     [Google Scholar]
  4. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants. Journal of Bacteriology 63:399–406
     [Google Scholar]
  5. Messer W., Vielmetter W. 1965; High resolution colony staining for the detection of bacterial growth requirement mutants using naphthol azo-dye techniques. Biochemical and Biophysical Research Communications 21:182–186
     [Google Scholar]
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A Cellophane Membrane Method for Screening Auxotrophic Mutants of Photochromogenic Mycobacteria
Microbiology 116, 253 (1980); https://doi.org/10.1099/00221287-116-1-253
/content/journal/micro/10.1099/00221287-116-1-253
/content/journal/micro/10.1099/00221287-116-1-253
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