1887

Abstract

The composition of extracellular polysaccharides produced by in defined culture media was compared with that of the polysaccharide present in the ooze produced during fireblight infection. The results strongly suggested that the ooze polysaccharide was of bacterial origin.

Two distinct polysaccharides were produced One contained galactose (61 to 69%), glucose (8 to 11%), mannose (2 to 6%) and uronic acid (16 to 23%) and the other contained only fructose. Both the quantity and composition of the polysaccharide produced was determined by the nature and concentration of the sugar or sugar alcohol supplied and by the nature of the nitrogen source.

Evidence is also presented which suggests that production of the ooze-like polysaccharide is a virulence determinant and that this polysaccharide is similar in composition to the capsule. Preliminary studies indicate the presence of capsule and polysaccharide-hydrolysing enzymes in lysates of phage-infected bacteria.

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1980-02-01
2024-05-06
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References

  1. Ayres S. H., Rupp P., Johnson W. T. 1919; A study of the alkali-forming bacteria in milk. United States Department of Agriculture Bulletin782
    [Google Scholar]
  2. Beer S. V., Aldwinckle H. S. 1976; Lack of correlation between susceptibility to Erwinia amylovora and sensitivity to amylovorin in apple cultivars. Proceedings of the American Phytopathological Society 3:300 abstract
    [Google Scholar]
  3. Beer S. V. J., Baker C. J., Woods A. C., Sjulen T. M. 1977; Amylovorin production in vitro and partial characterisation. Proceedings of the American Phytopathological Society 4:182–183 abstract
    [Google Scholar]
  4. Bennett R. A. 1978a Characteristics of the fireblight pathogen in relation to virulence Ph.D. thesis University of London;
    [Google Scholar]
  5. Bennett R. A. 1978b; Characteristics of Erwinia amylovora in relation to virulence. Proceedings of the IVth International Conference on Plant Pathogenic Bacteria II pp. 479–481
    [Google Scholar]
  6. Bennett R. A. 1980; Evidence for two virulence determinants in the fireblight pathogen Erwinia amylovora . Journal of General Microbiology 116:351–356
    [Google Scholar]
  7. Bennett R. A., Billing E. 1978a; Capsulation and virulence in Erwinia amylovora . Annals of Applied Biology 89:41–45
    [Google Scholar]
  8. Bennett R. A., Billing E. 1978b; Investigations on virulence determinants of Erwinia amylovora . Proceedings of the Illrd International Congress of Plant Pathology p. 69 abstract
    [Google Scholar]
  9. Billing E. 1960; An association between capsulation and phage sensitivity in Erwinia amylovora . Nature, London 186:819–820
    [Google Scholar]
  10. Eden-Green S. J. 1972 Studies in fireblight disease of apple, pear and hawthorn (Erwinia amylovora (Burrill) Winslow et al.) Ph.D. thesis University of London;
    [Google Scholar]
  11. Eden-Green S. J., Knee M. 1974; Bacterial polysaccharide in fireblight exudate. Journal of General Microbiology 81:509–512
    [Google Scholar]
  12. Goodman R. N., Huang J.-S., Huang P.-Y. 1974; Host-specific phytotoxic polysaccharide from apple tissue infected by Erwinia amylovora . Science 183:1081–1082
    [Google Scholar]
  13. Goodman R. N., Stoffl P. R., Ayers S. M. 1978; The utility of the fireblight toxin, amylovorin, for the detection of resistance of apple, pear and quince to Erwinia amylovora . Acta horticulturae 86:51–56
    [Google Scholar]
  14. Haber J. M. 1928; The relationship between Bacillus amylovorus and leaf tissue of apple. Pennsylvania Agricultural Experimental Station Bulletin2283–15
    [Google Scholar]
  15. Holligan P. M., Drew E. A. 1971; Routine analysis by gas-liquid chromatography of soluble carbohydrates in extracts of plant tissue. New Phytologist 70:271–279
    [Google Scholar]
  16. Hsu S.-T., Goodman R. N. 1978; Production of a host-specific, wilt inducing toxin in apple cell suspension cultures inoculated with Erwinia amylovora . Phytopathology 68:351–354
    [Google Scholar]
  17. Huang P.-Y., Goodman R. N. 1976; Ultrastructural modifications in apple stems induced by Erwinia amylovora and the fireblight toxin. Phytopathology 66:269–276
    [Google Scholar]
  18. Ivanoff S. S., Keitt G. W. 1941; Relations of nectar concentration to growth of Erwinia amylovora and fireblight infection of apple and pear blossoms. Journal of Agricultural Research 62:733–743
    [Google Scholar]
  19. Mccready R. M., Mccomb E. A. 1952; Extraction and determination of total pectic materials in fruits. Analytical Chemistry 24:1986–1988
    [Google Scholar]
  20. Miller P. W. 1929; Studies of fireblight of apple in Wisconsin. Journal of Agricultural Research 39:579–621
    [Google Scholar]
  21. Nixon E. L. 1927; The migration of Bacillus amylovorus in apple tissue and its effects on the host cells. Pennsylvania Agricultural Experimental Station Bulletin2123–16
    [Google Scholar]
  22. Saini A. S. 1966; Technical improvements in paper chromatography of sugars: method of sample desalting and sensitive staining reagent. Journal of Chromatography 24:484–486
    [Google Scholar]
  23. Schroth M. N., Thompson S. V., Hildebrand D. C., Moller W. J. 1974; Epidemiology and control of fireblight. Annual Review of Phytopathology 12:389–412
    [Google Scholar]
  24. Sjulin T. M., Beer S. V. 1978; Mechanism of wilt induction by amylovorin in cotoneaster shoots and its relation to wilting of shoots infected by Erwinia amylovora . Phytopathology 68:89–94
    [Google Scholar]
  25. Slade M. B., Tiffin A. I. 1978; Serological cross-reactions between Erwinia amylovora and Erwinia herhicola . Proceedings of the IVth International Conference on Plant Pathogenic Bacteria I pp. 289–294
    [Google Scholar]
  26. Thomas H. E., Ark P. A. 1934; Nectar and rain in relation to fireblight. Phytopathology 24:682–685
    [Google Scholar]
  27. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature, London 166:444–445
    [Google Scholar]
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