1887

Abstract

The genetic map of plasmid RP4 was correlated with its adenine + thymine (AT) map. For this purpose, RP4 DNA was digested with one or both of the restriction enzymes RI and dIII and the resulting linear RP4 molecules and fragments were partially denatured, examined in the electron microscope and their AT maps were determined using a computer program. From these AT maps the RI and dIII restriction sites were located on the AT map of RP4. Since the positions of these restriction sites on the genetic map of RP4 are known, the two maps could be compared. They revealed a high AT content for the Tn1 transposon and the kanamycin resistance gene. The region is also distinguished by a sharply defined AT-rich region, whereas and the tetracycline resistance gene have an AT content which is not distinctly different from the average AT content of RP4.

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/content/journal/micro/10.1099/00221287-117-1-135
1980-03-01
2024-04-27
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References

  1. Barth P.T., Grinter N.J. 1977; Map of plasmid RP4 derived by insertion of transposon C.. Journal of Molecular Biology 113:455–474
    [Google Scholar]
  2. Barth P.T., Grinter N.J., Bradley D. E. 1978; Conjugal transfer system of plasmid RP4: analysis by transposon 7 insertion.. Journal of Bacteriology 133:43–52
    [Google Scholar]
  3. Burkardt H.-J., Mattes R., Pühler A., Heumann W. 1978; Electron microscopy and computerized evaluation of some partially denatured group P resistance plasmids.. Journal of General Microbiology 105:51–62
    [Google Scholar]
  4. Burkardt H.-J., Riess G., Pühler A. 1979; Relationship of group PI plasmids revealed by heteroduplex experiments: RP1, RP4, R68 and RK2 are identical.. Journal of General Microbiology 114:341–348
    [Google Scholar]
  5. Datta N., Hedges R.W. 1972; Host ranges of R factors.. Journal of General Microbiology 70:453–460
    [Google Scholar]
  6. Datta N., Hedges R.W., Shaw E.J., Sykes R.B., Richmond M.H. 1971; Properties of an R factor from Pseudomonas aeruginosa.. Journal of Bacteriology 108:1244–1249
    [Google Scholar]
  7. Depicker A., v.Montagu M., Schell J. 1977; Physical map of RP4.. In DNA Insertion Elements, Plasmids and Episomes p. 678 Bukhari A.I., Shapiro J.A., Adhya S.L. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Gautier F., Mayer H., Goebel W. 1976; Cloning of calf thymus satellite I DNA in Escherichia coli.. Molecular and General Genetics 149:23–31
    [Google Scholar]
  9. Hedges R.W., Jacob A.E. 1974; Transposition of ampicillin resistance from RP4 to other replicons.. Molecular and General Genetics 132:31–40
    [Google Scholar]
  10. Holloway B.W., Richmond M.H. 1973; R factors used for genetic studies in strains of Pseudomonas aeruginosa and their origin.. Genetical Research 21:103–105
    [Google Scholar]
  11. Inman R.B. 1966; A denaturation map of the λ phage DNA molecule determined by electron microscopy.. Journal of Molecular Biology 18:464–476
    [Google Scholar]
  12. Kleinschmidt A., Zahn R.K. 1959; Über Desoxyribonucleinsäure-Molekeln in Protein-Mischfilmen.. Zeitschrift für Naturforschung 14b:770–779
    [Google Scholar]
  13. Lowbury E.J.L., Kidson A., Lilly H.A., Ayliffe G.A., Jones R.J. 1969; Sensitivity of Pseudomonas aeruginosa to antibiotics: emergence of strains highly resistant to carbenicillin.. Lancet ii:448–452
    [Google Scholar]
  14. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  15. Pühler A., Burkardt H.J. 1978; Fertility inhibition in Rhizobium lupini by the resistance plasmid RP4.. Molecular and General Genetics 162:163–171
    [Google Scholar]
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