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Volume 117, Issue 2

The Mitochondrial Cytochromes of an Unusual Budding Yeast, : Spectral Characterization Exploiting Fourth-order Finite Difference Analysis Free

Abstract

Low-temperature difference spectra of gradient-purified mitochondria from revealed the presence of and -type cytochromes, spectrally similar to those of other yeasts. Fourth-order finite difference analysis resolved the broad a-band of -and -type cytochromes into eight peaks. Absorption maxima at about 539, 543·5 and 547·5 nm were attributed to one, or perhaps two, cytochrome(s) that are loosely bound to the mitochondrial membrane. Cytochrome was identified at 5505 to 5515 nm. Maxima at about 554, 556, 559 and 562 nm were attributed to three or four distinct b-type cytochromes on the basis of their differential reduction by NADH, dithionite, or ascorbate plus ,-,-tetramethyl--phenylenediamine in the absence or presence of antimycin. Difference spectra in the presence of CO or cyanide indicated the presence of cytochromes (600 nm) and (608 nm). Finite difference analysis of the cytochromes oxidase peak centred at 600 nm revealed two components; the major component at 600 nm was identified as cytochrome and the minor component at 605 nm as cytochrome A further CO-binding haemoprotein was tentatively identified as cytochrome

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© Society for General Microbiology 1980
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1980-04-01
2024-05-03
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The Mitochondrial Cytochromes of an Unusual Budding Yeast, Sterigmatomyces halophilus: Spectral Characterization Exploiting Fourth-order Finite Difference Analysis
Microbiology 117, 315 (1980); https://doi.org/10.1099/00221287-117-2-315
/content/journal/micro/10.1099/00221287-117-2-315
/content/journal/micro/10.1099/00221287-117-2-315
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