1887

Abstract

, grown under culture conditions which led to unicell populations, was treated with -methyl-′-nitro--nitrosoguanidine and then cells were plated on rich medium to recover auxotrophic mutants. Carbon source and vitamin (-aminobenzoic acid, thiamin and nicotinamide)-requiring mutants were characterized. Amino acid-requiring mutants, some of types not previously isolated from green eukaryotes, were also obtained: 11 had a requirement for arginine, one for valine, two for threonine or isoleucine, and one for tryptophan, tyrosine or phenylalanine. The possible metabolic blocks of these amino acid-requiring mutants are discussed.

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/content/journal/micro/10.1099/00221287-119-1-273
1980-07-01
2024-05-19
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References

  1. Bachmann B. J., Low K. B., Taylor A. L. 1976; Recalibrated linkage map ofEscherichia coli K-12.. Bacteriological Reviews 40:116–167
    [Google Scholar]
  2. Fincham J. R. S., Day P. R., Radford A. 1979 Fungal Genetics, 4th edn.. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  3. Guardiola J. 1977; Requirements of the newly recognizedilvJ gene for the expression of valine transaminase activity inE. coli K-12.. Molecular and General Genetics 158:157–164
    [Google Scholar]
  4. Holliday R. 1956; A new method for the identification of biochemical mutants in microorganisms.. Nature; London: 178987
    [Google Scholar]
  5. Kirk D. L., Kirk M. M. 1978; Carriermediated uptake of arginine and urea byChlamydomonas reinhardi.. Plant Physiology 61:556–560
    [Google Scholar]
  6. Li S. L., Rédei G. P., Gowans C. S. 1967; A phylogenic comparison of mutation spectra.. Molecular and General Genetics 100:77–83
    [Google Scholar]
  7. Loppes R. 1969; A new class of arginine requiring mutants inChlamydomonas reinhardi.. Molecular and General Genetics 104:172–177
    [Google Scholar]
  8. Loppes R. 1970; Selection of arginine-requiring mutants inChlamydomonas reinhardi after treatment with three mutagens.. Experientia 26:660–661
    [Google Scholar]
  9. Mahanty H. K. 1979; Kanamycin-resistantScenedesmus obliquus showing simultaneous resistance to other antibiotics and polychlorinated biphenyls.. Nature; London: 277562–563
    [Google Scholar]
  10. Miflin B. J., Lea P. J. 1977; Amino acid metabolism.. Annual Review of Plant Physiology 28:299–329
    [Google Scholar]
  11. Powls R., Britton G. 1977; A series of mutant strains ofScenedesmus obliquus with abnormal carotenoid compositions.. Archives of Microbiology 113:275–280
    [Google Scholar]
  12. Trainor F. R., Roskowsky F. G. 1967; Control of unicell formation in a soilScenedesmus.. Canadian Journal of Botany 45:1657–1664
    [Google Scholar]
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