1887

Abstract

Microbiologically inapparent urogenital infection appeared to be induced in male guinea-pigs inoculated intra-urethrally with low doses of the guinea-pig inclusion conjunctivitis strain (GP-IC) of This state was indicated by the ability of inoculated animals to donate eye infection to normal animals caged with them. Donors failed to develop overt urogenital infection throughout the period of transmission judged by both absence of infected cells in urethral scrapings and failure to isolate GP-IC in cell culture; however, inoculation of donors with 5-iododeoxyuridine led to transient appearance of infectivity in scrapings. In distinction from overtly infected animals, donors failed to develop serum antibody and remained susceptible to urethral challenge with larger doses of GP-IC. Animals that had recovered from overt urethral infection were resistant to challenge and appeared unable to transmit eye infection.

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1980-08-01
2024-05-05
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References

  1. Allan I., Pearce J. H. 1979; Host modification of chlamydiae: differential infectivity for cell monolayers of chlamydiae grown in eggs and monolayers.. Journal of General Microbiology 112:53–59
    [Google Scholar]
  2. Allan I., Ainsworth S., Pearce J. H. 1979; Detection of low levels of chlamydial infectivity in cycloheximide-treated cell monolayers.. FEMS Microbiology Letters 5:385–388
    [Google Scholar]
  3. Barron A. L., White H. J., Rank R. G., Soloff B. L. 1979; Target tissues associated with genital infection of female guinea pigs by the chlamydial agent of guinea pig inclusion conjunctivitis.. Journal of Infectious Diseases 139:60–68
    [Google Scholar]
  4. Ganaway J. R. 1976; Bacterial, mycoplasma and rickettsial diseases.. In The Biology of the Guinea-Pig pp. 121–135 Wagner J. E., Manning P. J. Edited by New York, San Francisco & London:: Academic Press.;
    [Google Scholar]
  5. Hatch T. P. 1975; Competition between Chlamydia psittaci and L cells for host isoleucine pools: a limiting factor in chlamydial multiplication.. Infection and Immunity 12:211–220
    [Google Scholar]
  6. Howard L. V., O’Leary M. P., Nichols R. L. 1976; Animal model studies of genital chlamydial infections. Immunity to reinfection with guinea pig inclusion conjunctivitis agent in the urethra and eye of male guinea pigs.. British Journal of Venereal Diseases 52:261–265
    [Google Scholar]
  7. Kazdan J. J., Schachter J., Okumoto M. 1967; Inclusion conjunctivitis in the guinea pig.. American Journal of Ophthalmology 64:116–124
    [Google Scholar]
  8. Lamont H. C., Semine D. Z., Leveille C., Nichols R. L. 1978; Immunity to vaginal reinfection in female guinea pigs infected sexually with chlamydiae of guinea pig inclusion conjunctivitis.. Infection and Immunity 19:807–813
    [Google Scholar]
  9. Moore J. E., Griffiths M. S., Pearce J. H. 1974; Chlamydial infection of conjunctival tissues in culture.. British Journal of Experimental Pathology 55:396–405
    [Google Scholar]
  10. Mount D. T., Barron A. L. 1976; Intrarectal infection of guinea pigs with the agent of guinea pig inclusion conjunctivitis.. Proceedings of the Society for Experimental Biology and Medicine 153:388–391
    [Google Scholar]
  11. Mount D. T., Bigazzi P. E., Barron A. L. 1972; Infection of genital tract and transmission of ocular infection to newborns by the agent of guinea pig inclusion conjunctivitis.. Infection and Immunity 5:921–926
    [Google Scholar]
  12. Mount D. T., Bigazzi P. E., Barron A. L. 1973; Experimental genital infection of male guinea-pigs with the agent of guinea-pig inclusion conjunctivitis and transmission to females.. Infection and Immunity 8:925–930
    [Google Scholar]
  13. Murray E. S., Charbonnet L. T., Macdonald A. B. 1973; Immunity to chlamydial infection of the eye. I. The role of circulatory and secretory antibodies in resistance to reinfection with guinea pig inclusion conjunctivitis.. Journal of Immunology 110:1518–1525
    [Google Scholar]
  14. Nichols R. L., Murray E. S., Nisson P. E. 1978; Use of enteric vaccines in protection against chlamydial infections of the genital tract and the eye of guinea pigs.. Journal of Infectious Diseases 138:742–746
    [Google Scholar]
  15. Ozanne G., Pearce J. H. 1979; A model for latent chlamydial infection of the urogenital tract.. Journal of Medical Microbiology 12:xv
    [Google Scholar]
  16. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent end points.. American Journal of Hygiene 27:493–497
    [Google Scholar]
  17. Reeve P., Taverne J. 1962; A simple method for total particle counts of trachoma and inclusion blennorrhoea viruses.. Nature; London: 195923–924
    [Google Scholar]
  18. Richmond S. J., Clarke S. K. R. 1977; Problems in assigning a causative role to chlamydiae isolated in nongonococcal urethritis.. In Nongonococcal Urethritis and Related Infections pp. 43–46 Hobson D., Holmes K. K. Edited by Washington, D.C.:: American Society for Microbiology.;
    [Google Scholar]
  19. Schachter J. 1978; Chlamydial infections.. New England Journal of Medicine 298:428–435
    [Google Scholar]
  20. Storz J. 1971 Chlamydia and Chlamydia-induced Diseases. Springfield, Illinois:: C. C. Thomas.;
    [Google Scholar]
  21. Treharne J. D. 1971; A comparison of microbiological techniques for the detection of GP-IC agent in the conjunctiva of infected guinea pigs.. In Trachoma and Related Disorders pp. 435–444 Nichols R. L. Edited by Amsterdam:: Excerpta Medica.;
    [Google Scholar]
  22. Wang S. P. 1971; A microimmunofluorescence method. Study of antibody response to TRIC organisms in mice.. In Trachoma and Related Disorders pp. 273–288 Nichols R. L. Edited by Amsterdam:: Excerpta Medica.;
    [Google Scholar]
  23. Woodland R. M., El-Sheikh H., Darougar S., Squires S. 1978; Sensitivity of immuno-peroxidase and immunofluorescence staining for detecting chlamydia in conjunctival scrapings and in cell culture.. Journal of Clinical Pathology 31:1073–1077
    [Google Scholar]
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