1887

Abstract

Both a penicillinase and a cephalosporinase are present in a strain of (GN7391) resistant to -lactam antibiotics. The penicillinase was identical to the type Ia penicillinases (Type III by Richmond classification), mediated by Rms212 and R-TEM. A cephalosporinase, typical of enterobacteriaceae chromosomal -lactamase (Type I by Richmond classification), was purified from the strain. It gave a single protein band on polyacrylamide gel electrophoresis and immunoelectrophoresis; the pI was 8·6 and its molecular weight was approximately 38000. Cysteine was not found among its amino acids. The specific activity was 388 units (mg protein)for the hydrolysis of cephaloridine, and the optimal pH was 8·0. Rabbit antiserum obtained against the purified enzyme showed cross-reaction with cephalosporinases produced by strains of in a neutralization test.

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1980-12-01
2024-04-28
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References

  1. Andrews P. 1964; Estimation of the molecular weight of proteins by Sephadex gel filtration. Biochemical Journal 91:222–233
    [Google Scholar]
  2. Bill N. J., Washington J. A. II 1977; Comparison of in vitro activity of cephalexin, cephradine, and cefaclor. Antimicrobial Agents and Chemotherapy 11:470–474
    [Google Scholar]
  3. Cowan S. T., Steel K. J. 1974 Manual for the Identification of Medical Bacteria, 2nd edn. Cambridge: Cambridge University Press;
    [Google Scholar]
  4. Davis B. J. 1964; Disc electrophoresis II. Method and application to human serum proteins. Annals of the New York Academy of Sciences 121:404–427
    [Google Scholar]
  5. Dubois M., Gilles K. A., Hamilton J. K., Rebers P. A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  6. Fujii-Kuriyama Y., Yamamoto M., Sugawara S. 1977; Purification and properties of beta-lactamase from Proteus morganii. Journal of Bacteriology 131:726–734
    [Google Scholar]
  7. Jack G. W., Richmond M. H. 1970; A comparative study of eight distinct β-lactamases synthesized by Gram-negative bacteria. Journal of General Microbiology 61:43–61
    [Google Scholar]
  8. Letarte R., Felix M. D., Pechere J. C., Leprohon D. A. 1977; Enzymatic and immunological characterization of a new cephalosporinase from Enterobacter aerogenes. Antimicrobial Agents and Chemotherapy 12:201–205
    [Google Scholar]
  9. Matthew M., Harris A. M. 1976; Identification of β-lactamases by analytical isoelectric focusing: correlation with bacterial taxonomy. Journal of General Microbiology 94:55–67
    [Google Scholar]
  10. Minami S., Inoue M., Mitsuhashi S. 1979; Purification and properties of β-lactamase from Enterobacter cloacae. In Proceedings of the 8 th Gunma Symposium on Microbial Drug Resistance pp. 52–53 Edited by Mitsuhashi S. Gunma: Gunma University Press;
    [Google Scholar]
  11. Newsom S. W. B., Marshall M. J., Harris A. M. 1974; Enterobacteria, β-lactam antibiotics and β-lactamases in clinical practice. Journal of Medical Microbiology 7:473–482
    [Google Scholar]
  12. O’Callaghan C. H., Muggleton P. W., Ross G. W. 1969; Effects of β-lactamase from Gram-negative organisms on cephalosporins and penicillins. In Antimicrobial Agents and Chemotherapy-1968 pp. 55–63 Edited by Hobby G. L. Maryland: American Society for Microbiology;
    [Google Scholar]
  13. Richmond M. H., Sykes R. B. 1973; The β-lactamases of Gram-negative bacteria and their possible physiological role. Advances in Microbial Physiology 9:31–88
    [Google Scholar]
  14. Sawai T., Mitsuhashi S., Yamagishi S. 1968; Drug resistance of enteric bacteria. XIV. Comparison of β-lactamases in Gram-negative rod bacteria resistant to α-aminobenzylpenicillin. Japanese Journal of Microbiology 12:423–434
    [Google Scholar]
  15. Sawai T., Takahashi K., Yamagishi S., Mitsuhashi S. 1970a; Variant of penicillinase mediated by an R factor in Escherichia coli. Journal of Bacteriology 104:620–629
    [Google Scholar]
  16. Sawai T., Mitsuhashi S., Yamagishi S. 1970b; Stability of various derivatives of 7-aminocephalosporanic acid and 6-aminopenicillanic acid to cephalosporinases from Gram-negative bacteria. In Progress in Antimicrobial and Anticancer Chemotherapy 1 pp. 410–415 Edited by Umezawa H. Tokyo: University of Tokyo Press;
    [Google Scholar]
  17. Spackman D. H., Stein W. H., Moore S. 1958; Automatic recording apparatus for use in the chromatography of amino acid. Analytical Chemistry 30:1190–1206
    [Google Scholar]
  18. Sykes R. B., Matthew M. 1976; The β-lactamases of Gram-negative bacteria and their role in resistance to β-lactam antibiotics. Journal of Antimicrobial Chemotherapy 2:115–157
    [Google Scholar]
  19. Tajima M., Takenouchi Y., Sugawara S. 1979; Purification and properties of β-lactamases from Citrobacter freundii. In Proceedings of the 8th Gunma Symposium on Microbial Drug Resistance pp. 56–57 Edited by Mitsuhashi S. Gunma: Gunma University Press;
    [Google Scholar]
  20. Verbist L. 1976; Comparison of the antibacterial activity of nine cephalosporins against Enterobacteriaceae and nonfermentative Gram-negative bacilli. Antimicrobial Agents and Chemotherapy 10:657–663
    [Google Scholar]
  21. Waley S. G. 1974; A spectrophotometric assay of β-lactamase action on penicillins. Biochemical Journal 139:789–790
    [Google Scholar]
  22. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  23. Yamanaka Y., Tajima M., Nakazawa S. 1973; Purification and characterization of β-lactamase from clinically isolated E. coli strain No. 24. Chemotherapy, Tokyo 21:1179–1183
    [Google Scholar]
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