1887

Abstract

The mode of action of nosiheptide (multhiomycin) on bacterial protein synthesis is closely similar to that of thiostrepton. Both antibiotics inhibit functions of elongation factors Tu and G and greatly reduce the synthesis of guanosine penta- and tetraphosphates in response to stringent factor. Furthermore, the actinomycetes which produce these antibiotics defend themselves against their products in similar fashion. This involves specific pentose-methylation of 23S ribosomal RNA.

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1981-09-01
2024-04-27
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References

  1. Block R., Haseltine W. A. 1975; Purification and properties of stringent factor. Journal of Biological Chemistry 250:1212–1217
    [Google Scholar]
  2. Bodley J. W., Lin L., Highland J. H. 1970; Studies on translocation. VI: Thiostrepton prevents the formation of a ribosome-G factor-guanine nucleotide complex. Biochemical and Biophysical Research Communications 41:1406–1411
    [Google Scholar]
  3. Cundliffe E. 1978; Mechanism of resistance to thiostrepton in the producing-organism Streptomyces azureus. Nature; London: 272792–795
    [Google Scholar]
  4. Cundliffe E. 1980; Antibiotics and prokaryotic ribosomes: action, interaction, and resistance. In Ribosomes: Structure, Function and Genetics pp. 555–581 Chambliss G., Craven G. R., Davies J., Davis K., Kahan L., Nomura M. Edited by Baltimore: University Park Press.;
    [Google Scholar]
  5. Cundliffe E., Dixon P. D. 1975; Inhibition of ribosomal A site functions by sporangiomycin and micrococcin. Antimicrobial Agents and Chemotherapy 8:1–4
    [Google Scholar]
  6. Cundliffe E., Thompson J. 1979; Ribose methylation and resistance to thiostrepton. Nature; London: 278859–861
    [Google Scholar]
  7. Cundliffe E., Dixon P., Stark M., Stöffler G., Ehrlich R., Stöffler-Meilicke M., Cannon M. 1979; Ribosomes in thiostrepton-resistant mutants of Bacillus megaterium lacking a single 50S subunit protein. Journal of Molecular Biology 132:235–252
    [Google Scholar]
  8. Dijk J., Littlechild J. 1979; Purification of ribosomal proteins from Escherichia coli under non-denaturing conditions. Methods in Enzymology 59:481–502
    [Google Scholar]
  9. Pedersen F. S., Lund E., Kjeldgaard N. O. 1973; Codon specific, tRNA dependent in vitro synthesis of ppGpp and pppGpp. Nature New Biology 243:13–15
    [Google Scholar]
  10. Pestka S., Brot N. 1971; Studies on the formation of transfer RNA-ribosome complexes. XV. Effect of antibiotics on steps of bacterial protein synthesis: some new ribosomal inhibitors of translocation. Journal of Biological Chemistry 246:7715–7722
    [Google Scholar]
  11. Prange T., Ducruix A., Pascard C., Lunel J. 1977; Structure of nosiheptide, a polythiazole containing antibiotic. Nature; London: 265189–190
    [Google Scholar]
  12. Stark M., Cundliffe E. 1979a; Requirement for ribosomal protein BM-L11 in stringent control of RNA synthesis in Bacillus megaterium. European Journal of Biochemistry 102:101–105
    [Google Scholar]
  13. Stark M., Cundliffe E. 1979b; On the biological role of ribosomal protein BM-L11 of Bacillus megaterium, homologous with Escherichia coli ribosomal protein L11. Journal of Molecular Biology 134:767–779
    [Google Scholar]
  14. Tamura A., Furuta R., Naruto S., Ishii H. 1973; Actinotiocin, a new sulphur-containing peptide antibiotic from Actinomadura pusilla. Journal of Antibiotics 26:343–350
    [Google Scholar]
  15. Tanaka T., Endo T., Shimazu A., Yoshida R., Suzuki Y., Otake N., Yonehara H. 1970a; A new antibiotic, multhiomycin. Journal of Antibiotics 23:231–237
    [Google Scholar]
  16. Tanaka T., Sakaguchi K., Yonehara H. 1970b; On the mode of action of multhiomycin. 1. Effects of multhiomycin on macromolecular syntheses. Journal of Antibiotics 23:401–407
    [Google Scholar]
  17. Tanaka T., Sakaguchi K., Yonehara H. 1971; Inhibition by multhiomycin of T factor- and GTP-dependent binding of phenylalanyl-tRNA to ribosomes and GTP hydrolysis associated with it. Journal of Biochemistry 69:1127–1130
    [Google Scholar]
  18. Thompson J., Cundliffe E. 1980; Resistance to thiostrepton, siomycin and sporangiomycin in actinomycetes that produce them. Journal of Bacteriology 142:455–461
    [Google Scholar]
  19. Thompson J., Cundliffe E. 1981; Purification and properties of an RNA methylase produced by Streptomyces azureus and involved in resistance to thiostrepton. Journal of General Microbiology 124:291–297
    [Google Scholar]
  20. Thompson J., Cundliffe E., Stark M. 1979; Binding of thiostrepton to a complex of 23S rRNA with ribosomal protein L11. European Journal of Biochemistry 98:261–265
    [Google Scholar]
  21. Wagner E. G. H., Kurland C. G. 1980; Escherichia coli elongation factor G blocks stringent factor. Biochemistry 19:1234–1240
    [Google Scholar]
  22. Weisblum B., Demohn V. 1970; Inhibition by thiostrepton of the formation of a ribosome-bound guanine nucleotide complex. FEBS Letters 11:149–152
    [Google Scholar]
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