1887

Abstract

Mitochondria from conidia of were isolated and purified by a simple and rapid technique. Electron micrographic analysis and isopycnic centrifugation on a sucrose density gradient revealed a heterogeneous population.

Succinate, citrate, 2-oxoglutarate, NADH and ascorbate + tetramethyl--phenylenediamine (TMPD) were oxidized by the mitochondria. Oxygen consumption was totally inhibited by antimycin A or cyanide. The electron transport chain was coupled to sites 1 and 2 of phosphorylation. Oligomycin, atractyloside and 2,4-dinitrophenol prevented energy coupling.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-127-2-309
1981-12-01
2024-05-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/127/2/mic-127-2-309.html?itemId=/content/journal/micro/10.1099/00221287-127-2-309&mimeType=html&fmt=ahah

References

  1. Brambl R. 1981; Respiration and mitochondrial biogenesis during fungal spore germination. In The Fungal Spore: Morphogenetic Controls pp. 585–604 Edited by Turian G., Hohl H. London: Academic Press.;
    [Google Scholar]
  2. Chance B., Williams G.R. 1955; A simple and rapid assay of oxidative phosphorylation. Nature, London 175:1120–1121
    [Google Scholar]
  3. Colvin H.J., Sauer B.L., Munkres K.D. 1973; Respiration of wild type and extrachromosomal mutants of Neurospora crassa. Journal of Bacteriology 116:1314–1321
    [Google Scholar]
  4. Coruzzi G., Trembath M. K., Tzagoloff A. 1979; The isolation of mitochondrial and nuclear mutants of Saccharomyces cerevisiae with specific defects in mitochondrial functions. Methods in Enzymology 56:95–106
    [Google Scholar]
  5. Gautheron D.C. 1973; Existe-t-il des critéres et des propriétés permettant de discriminer entre les différents groupements thiols impliqués dans les translocations mitochondriales et le mécanisme de coupiage?. Biochimie 55:727–745
    [Google Scholar]
  6. Greenawalt J.W., Beck D.P., Hawley E.S. 1972; Chemical and biochemical changes in mitochondria during morphogenetic development of Neurospora crassa. In Biochemistry and Biophysics of Mitochondrial Membranes pp. 541–558 Edited by Azzone J. F., Carafoli A. P. New York: Academic Press.;
    [Google Scholar]
  7. Hall D.O., Greenawalt J.W. 1964; Oxidative phosphorylation by isolated mitochondria. Biochemical and Biophysical Research Communications 17:565–569
    [Google Scholar]
  8. Hall D.O., Greenawalt J.W. 1967; The preparation and biochemical properties of mitochondria from Neurospora crassa. Journal of General Microbiology 48:419–430
    [Google Scholar]
  9. Klingenberg M. 1976; The ADP-ATP carrier in mitochondrial membranes. In Enzymes of Biological Membranes 3 pp. 383–438 Edited by Martonosi A. New York: Plenum Publishing Corp;
    [Google Scholar]
  10. Lambowitz A.M., Smith E.W., Slayman C. W. 1972a; Electron transport in Neurospora mitochondria. Journal of Biological Chemistry 247:4850–4858
    [Google Scholar]
  11. Lambowitz A.M., Smith E.W., Slayman C.W. 1972b; Oxidative phosphorylation in Neurospora mitochondria. Journal of Biological Chemistry 247:4859–4865
    [Google Scholar]
  12. Lloyd D. 1979; General methodology for isolation and characterization of mitochondria from micro-organisms. Methods in Enzymology 55:135–144
    [Google Scholar]
  13. Michéa-Hamzehpour M., Ortega Perez R., Rougemont A., Vanderhaeghe F., Khandjian E., Ton That T.C., Turian G. 1979; Isolation of two mitochondrial populations with differential hydroxamate-sensitivity from the poky mutant of Neurospora crassa. FEMS Microbiology Letters 6:213–218
    [Google Scholar]
  14. Michéa-Hamzehpour M., Grange F., Ton That T.C., Turian G. 1980; Heat-induced changes in respiratory pathways and mitochondrial structure during microcycleconidiation of Neurospora crassa. Archives of Microbiology 125:53–58
    [Google Scholar]
  15. Ortega Perez R.D., Khandjian E.W., Rougemont A., Ojha M., Turian G. 1977; Changes of mitochondrial density during differentiation of Neurospora crassa. FEMS Microbiology Letters 2:207–209
    [Google Scholar]
  16. Spurr A.R. 1969; A low viscosity epoxy resin embedding medium for electron microscopy. Journal of Ultrastructural Research 26:31–43
    [Google Scholar]
  17. Von Jagow G., Weiss H., Klingenberg M. 1973; Comparison of the respiratory chain of Neurospora crassa wild type and the mi-mutants mi-l and mi-3. European Journal of Biochemistry 33:140–157
    [Google Scholar]
  18. Weiss B., Turian G. 1966; A study of conidiation in Neurospora crassa. Journal of General Microbiology 44:407–418
    [Google Scholar]
  19. Weiss H., Von Jagow G., Klingenberg M., Bücher T. 1970; Characterization of Neurospora crassa mitochondria prepared with a grind-mill. European Journal of Biochemistry 14:75–82
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-127-2-309
Loading
/content/journal/micro/10.1099/00221287-127-2-309
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error