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Volume 128, Issue 1

Colicins E4, E5, E6 and A and Properties of Colicinogenic Transconjugants Free

Abstract

E colicins are those inactive on mutants, which lack the outer membrane protein that adsorbs vitamin B12, E colicins and phage BF23; types E1, E2, E3 and E7 have been defined by the specific immunity of E-colicinogenic strains to the type of E colicin they produce. Further immunity types - E4, E5 and E6 - are now described. of colicin type 9 makes colicin E4, and of types 9A, 12 and 14 make colicin E6 (not colicin E3, as previously supposed). Many local isolates make E colicin of a new type - E5. The action of colicins E1 to E7 was (incompletely) blocked by vitamin B12, which also reduced the effect of A colicins (which are weakly active on K12 indicators). K12 given plasmid ColA-23 by transformation was immune to A colicins but sensitive to colicins E1 to E7. A purported ‘colicin E4’ was shown to be of class colicin A. K12(CloDF13) transformants were immune to colicin E6. A ‘cap’ of colicin-sensitive indicator bacteria developed over, but not around, killed colonies of E-colicinogenic strains, because of adsorption of colicin by non-induced bacteria of the colony. Killed colonies of E-colicinogenic strains gave partly discrete lobes of colicin action on indicator lawns, instead of circular zones, apparently as a result of unstable variation in colicin production. The presence of a colicin E4, E5 or E6 plasmid in K12 strains made them more sensitive to colicins E2 and E7, as shown by zones of partial translucency surrounding the ordinary colicin zones and by increased titre of colicin E2 and E7 preparations on such indicators.

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© Society for General Microbiology, 1982
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1982-01-01
2024-05-03
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References

  1. Abbott J. D., Shannon R. 1958; A method for typing Shigella sonnei, using colicine production as a marker. Journal of Clinical Pathology 11:71–77
     [Google Scholar]
  2. Barry G. T., Everhart D. L., Graham M. 1963; Colicin A. Nature, London 198:211–213
     [Google Scholar]
  3. Cavard D., Lazdunski C. J. 1979; Purification and molecular properties of a new colicin. European Journal of Biochemistry 96:519–524
     [Google Scholar]
  4. Davies J. K., Reeves P. 1975; Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A. Journal of Bacteriology 123:102–117
     [Google Scholar]
  5. Dimasi D. R., White J. C., Schnaitman C. A., Bradbeer C. 1973; Transport of vitamin B12 in Escherichia coli:common receptor sites for vitamin B12 and the E colicins on the outer-membrane of the cell envelope. Journal of Bacteriology 115:506–513
     [Google Scholar]
  6. Fredericq P. 1949; Sur la résistance croisée entre colicine E et bacteriophage II. Comptesrendus des seances de la Société de Biologieet de ses Filiales 143:1011–1013
     [Google Scholar]
  7. Fredericq P. 1956; Résistance etimmunité aux colicines. Comptesrendus des séances de la Société de Biologieet de ses Filiales 150:1514–1517
     [Google Scholar]
  8. Fredericq P. 1965; A note on the classification of colicins. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 196:140–142
     [Google Scholar]
  9. Graham A. G. 1974 Ph.D. thesis: Stanford University, U.S.A;
  10. Hamon Y., Peron Y. 1964; Description de sept nouveaux types de colicines. Etatactuel de la classification de cesantibiotiques. Annales de l’Institut Pasteur 107:44–54
     [Google Scholar]
  11. Hill C., Holland I. B. 1967; Genetic basis of colicin E susceptibility in Escherichia coli. I. Refractory mutants. Journal of Bacteriology 94:677–686
     [Google Scholar]
  12. Horak V. 1975; Typing of Shigella sonnei colicins by means of specific indicators. Zentralblatt für Bakteriologie, Parasitenkunde, Infektions-krankheiten und Hygiene(Abteilung I, Originate A) 233:58–63
     [Google Scholar]
  13. Inselburg J. 1974; Incompatibility exhibited by colicin plasmids El, E2 and E3 in Escherichia coli. Journal of Bacteriology 119:478–483
     [Google Scholar]
  14. Inselburg J. 1979; Studies of colicin induction with an imm− col+ mutant of the plasmid colicin El. Molecular and General Genetics 173:333–338
     [Google Scholar]
  15. Kadner R. J. 1978; Repression of synthesis of the vitamin B12 receptor in Escherichia coli. Journal of Bacteriology 136:1050–1057
     [Google Scholar]
  16. Kadner R. J., Bassford P. J.JR Pugsley A. P. 1979; Colicin receptors and the mechanisms of colicin uptake. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 244:90–104
     [Google Scholar]
  17. Kirschbaum J. B., Konrad E. B. 1973; Isolation of a specialized lambda transducing phage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase. Journal of Bacteriology 116:517–526
     [Google Scholar]
  18. Kool A. J., Pols C., Nijkamp H. J. J. 1975; Bacteriocinogenic CloDF13 minicells of Escherichia coli synthesize a protein that accounts for immunity to bacteriocinClo DF13: purification and characterization of the immunity protein. Antimicrobial Agents and Chemotherapy 8:67–75
     [Google Scholar]
  19. Lewis M. J., Stocker B. A. D. 1965; Properties of some group E colicine factors. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrank-heiten und Hygiene (Abteilung I, Originate A) 196:173–183
     [Google Scholar]
  20. Males B. M., Stocker B. A. D. 1980; Escherichia coli K317, formerly used to define colicin group E2, produces colicin E7, is immune to colicin E2, and carries a bacteriophage-restricting conjugative plasmid. Journal of Bacteriology 144:524–531
     [Google Scholar]
  21. Ozeki H., Stocker B. A. D., De Margerie H. 1959; Production of colicine by single bacteria. Nature, London 184:337–339
     [Google Scholar]
  22. Ozeki H., Stocker B. A. D., Smith S. M. 1962; Transmission of colicinogeny between strains of Salmonella typhimurium grown together. Journal of General Microbiology 28:671–687
     [Google Scholar]
  23. Van Vught A.M.J.J., Delamarre E. C. M. 1977; Genetics of colicin E susceptibility in Citrobacter freundii. Antonie van Leeuwenhoek 47:7–18
     [Google Scholar]
  24. Vosti K. L. 1968; Production and sensitivity to colicins among serologically classified strains of Escherichia coli. Journal of Bacteriology 96:1947–1952
     [Google Scholar]
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Colicins E4, E5, E6 and A and Properties of btuB+ Colicinogenic Transconjugants
Microbiology 128, 95 (1982); https://doi.org/10.1099/00221287-128-1-95
/content/journal/micro/10.1099/00221287-128-1-95
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