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Volume 128, Issue 11

Phages Iα and I-2: IncI Plasmid-dependent Bacteriophages Free

Abstract

Phage Iα was isolated from sewage from Windhoek, South West Africa. It formed relatively clear plaques about 2 mm in diameter, on sensitive strains of K12 and LT2. The phage had an hexagonal outline with a diameter of about 24 nm, contained RNA and was resistant to chloroform. Phage Iα formed plaques or propagated only on organisms carrying I plasmids or the Iγ plasmid R621a. The efficiency of plating was higher on than on hosts. The phage adsorbed along the length of shafts of I pili.

Phage I-2 was isolated from Pretoria sewage. It was a filamentous virus and individual virions varied considerably in length. Phage I-2 formed turbid plaques which varied from pin point to about 1 mm in diameter on all hosts. It was resistant to RNAase and sensitive to chloroform. Phage I-2 had a spectrum of activity limited to strains harbouring I plasmids but the adsorption site could not be demonstrated. The phage was not related serologically to phages If1 or PR64FS.

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© Society for General Microbiology 1982
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1982-11-01
2024-05-21
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References

  1. Bradley D. E. 1977; Characterization of pili determined by drug resistance plasmids R711b and R778b. Journal of General Microbiology 102:349–363
     [Google Scholar]
  2. Bradley D. E. 1978; Pseudomonas aeruginosa pili. In Pili pp. 319–338 Bradley D. E., Raizen E., Fives-Taylor P., Ou J. Edited by Washington, D.C: International Conferences on Pili;
     [Google Scholar]
  3. Bradley D. E. 1980a; Determination of pili by con- jugative bacterial drug resistance plasmids of incompatibility groups B, C, H, J, K, M, V and X. Journal of Bacteriology 141:828–837
     [Google Scholar]
  4. Bradley D. E. 1980b; Morphological and serological relationships of conjugative pili. Plasmid 4:155–169
     [Google Scholar]
  5. Bradley D. E., Rutherford E. L. 1975; Basic characterization of a lipid-containing bacteriophage specific for plasmids of the P, N, and W compatibility groups. Canadian Journal of Microbiology 21:152–163
     [Google Scholar]
  6. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981a; Phage X: a plasmid- dependent, broad host range, filamentous bacterial virus. Journal of General Microbiology 126:389–396
     [Google Scholar]
  7. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981b; Phage t: a group T plasmid- dependent bacteriophage. Journal of General Microbiology 126:397–403
     [Google Scholar]
  8. Bradley D. E., Coetzee J. N., Bothma T., Hedges R. W. 1981c; Phage F0lac: an F0 lac plasmid-dependent bacteriophage. Journal of General Microbiology 126:405–411
     [Google Scholar]
  9. Bradley D. E., Sirgel F. A., Coetzee J. N., Hedges R. W., Coetzee W. F. 1982; Phages C- 2 and J: IncC and IncJ plasmid-dependent phages, respectively. Journal of General Microbiology 128:2485–2498
     [Google Scholar]
  10. Coetzee J. N. 1964; Transmission of colicinogeny to Providence strains. Nature; London: 203897–898
     [Google Scholar]
  11. Coetzee J. N., Lecatsas G., Coetzee W. F., Hedges R. W. 1979; Properties of R plasmid R772 and the corresponding pilus-specific phage PR772. Journal of General Microbiology 110:263–273
     [Google Scholar]
  12. Coetzee J. N., Sirgel F. A., Lecatsas G. 1980; Properties of a filamentous phage which adsorbs to pili coded by plasmids of the IncI complex. Journal of General Microbiology 117:547–551
     [Google Scholar]
  13. Datta N. 1977; Classification of plasmids as an aid to understanding their epidemiology and evolution. Journal of Antimicrobial Chemotherapy 3: suppl. C 19–23
     [Google Scholar]
  14. Datta N. 1979; Plasmid classification: incompatibility grouping. In Plasmids of Medical, Environmental and Commercial Importance pp. 3–12 Timmis K. M., Puhler A. Edited by Amsterdam: Elsevier-/North Holland Biomedical Press;
     [Google Scholar]
  15. Datta N., Barth P. T. 1976; Compatibility properties of R483, a member of the I plasmid complex. Journal of Bacteriology 125:796–799
     [Google Scholar]
  16. Datta N., Hedges R. W. 1972; Host ranges of R factors. Journal of General Microbiology 70:453–460
     [Google Scholar]
  17. Datta N., Hedges R. W. 1973; An I pilus-determining R factor with anomalous compatibility properties, mobilizing a gentamicin-resistance plasmid. Journal of General Microbiology 77:11–17
     [Google Scholar]
  18. Datta N., Olarte J. 1974; R factors in strains of Salmonella typhi Shigella dysenteriae 1 isolated during epidemics in Mexico: classification by compatibility. Antimicrobial Agents and Chemotherapy 5:310–317
     [Google Scholar]
  19. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. 1971; Properties of an R factor from Pseudomonas aeruginosa. Journal of Bacteriology 108:1244–1249
     [Google Scholar]
  20. Dennison S., Hedges R. W. 1972; Host specificities of RNA phages. Journal of Hygiene 70:55–61
     [Google Scholar]
  21. Dowman J. E., Meynell G. G. 1970; Pleiotropic effects of derepressed bacterial sex factors on colicin- ogeny and cell wall structure. Molecular and General Genetics 109:57–68
     [Google Scholar]
  22. Falkow S., Guerry P., Hedges R. W., Datta N. 1974; Polynucleotide sequence relationships among plasmids of the I compatibility complex. Journal of General Microbiology 85:65–76
     [Google Scholar]
  23. Grant R. B., Whiteley M. H., Shapley A. J. 1978; Plasmids of incompatibility group P code for the capacity to propagate bacteriophage IKe. Journal of Bacteriology 136:808–811
     [Google Scholar]
  24. Gray C. W., Brown R. S., Marvin D. A. 1981; Adsorption complex of filamentous fd virus. Journal of Molecular Biology 146:621–627
     [Google Scholar]
  25. Grindley N. D. F., Humphreys G. O., Anderson E. S. 1973; Molecular studies of R factor compatibility groups. Journal of Bacteriology 115:387–389
     [Google Scholar]
  26. Grindley N. D. F., Humphreys G. O., Anderson E. S. 1973; Molecular studies of R factor compatibility groups. Journal of Bacteriology 115:387–389
     [Google Scholar]
  27. Hedges R. W. 1974; R factors from Providence. Journal of General Microbiology 81:171–181
     [Google Scholar]
  28. Hedges R. W. 1975; R factors from Proteus mirabilis and P. vulgaris. Journal of General Microbiology 87:301–311
     [Google Scholar]
  29. Hedges R. W., Datta N. 1973; Plasmids determining I pili constitute a compatibility complex. Journal of General Microbiology 77:19–25
     [Google Scholar]
  30. Hedges R. W., Datta N., Kontamichalou P., Smith J. T. 1974; Molecular specificities of R factor-determined beta-lactamases: correlation with plasmid compatibility. Journal of Bacteriology 117:56–62
     [Google Scholar]
  31. Jacob A. E., Shapiro J. A., Yamamoto L., Smith D. I., Cohen S. N., Berg D. 1977; Plasmids studied in Escherichia coli and other enteric bacteria. In DNA Insertion Elements, Plasmids and Episomes pp. 607–638 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  32. Jobanputra R. S., Datta N. 1974; Trimethoprim R factors in enterobacteria from clinical specimens. Journal of Medical Microbiology 7:169–177
     [Google Scholar]
  33. Lawn A. M., Meynell E. 1970; Serotypes of sex pili. Journal of Hygiene 68:683–694
     [Google Scholar]
  34. Lawn A. M., Meynell E., Meynell G. G., Datta N. 1967; Sex pili and the classification of sex factors in the Enterobacteriaceae. Nature; London: 216: 343–346
     [Google Scholar]
  35. Meynell E. 1978; Experiments with sex pili: an investigation of the characters and function of F-like and I-like sex pili based on their reactions with antibody and phage. In Pili pp. 207–233 Bradley D. E., Raizen E., Fives-Taylor P., Ou J. Edited by Washington, D.C: International Conferences on Pili;
     [Google Scholar]
  36. Meynell E., Datta N. 1967; Mutant drug resistance factors of high transmissibility. Nature; London: 214885–887
     [Google Scholar]
  37. Meynell E., Meynell G. G., Datta N. 1968; Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids. Bacteriological Reviews 32:55–83
     [Google Scholar]
  38. Meynell G. G. 1973 Bacterial Plasmids p. 55 London: Macmillan;
     [Google Scholar]
  39. Meynell G. G., Lawn A. M. 1968; Filamentous phages specific for the I sex factor. Nature; London: 218 1184–1186
     [Google Scholar]
  40. Miyake T., Shiba T., Sakurai T., Watanabe I. 1969; Isolation and properties of two new RNA phages SP and FI. Japanese Journal of Microbiology 13:375–382
     [Google Scholar]
  41. Olsen R. H., Thomas D. D. 1973; Characteristics and purification of PRR1, an RNA phage specific for the broad host range Pseudomonas R1822 drug resistance plasmid. Journal of Virology 12:1560–1567
     [Google Scholar]
  42. Romero E., Riva S., Fietta A. M., Silvestri L. G. 1971; Effect of R factors on rifampicin resistance in E. coli. Nature New Biology 234:56–58
     [Google Scholar]
  43. Salisbury B., Hedges R. W., Datta N. 1972; Two modes of ‘curing’ transmissible bacterial plasmids. Journal of General Microbiology 70:443–452
     [Google Scholar]
  44. Slrgel F. A., Coetzee J. N., Hedges R. W., Lecatsas G. 1981; Phage C-l: an IncC group plasmid-specific phage. Journal of General Microbiology 122:155–160
     [Google Scholar]
  45. Spratt B. G., Rowbury R. H., Meynell G. G. 1973; The plasmid of Salmonella typhimurium LT2. Molecular and General Genetics 121:347–353
     [Google Scholar]
  46. Stanisich V. A. 1974; The properties and host range of male-specific bacteriophages of Pseudomonas aeruginosa. Journal of General Microbiology 84:332–342
     [Google Scholar]
  47. Towner K. H. 1979; Classification of transferable plasmids conferring resistance to trimethoprim isolated in Great Britain. FEMS Microbiology Letters 5:319–321
     [Google Scholar]
  48. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology 40:734–744
     [Google Scholar]
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Phages Iα and I2-2: IncI Plasmid-dependent Bacteriophages
Microbiology 128, 2797 (1982); https://doi.org/10.1099/00221287-128-11-2797
/content/journal/micro/10.1099/00221287-128-11-2797
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