1887

Abstract

was cultivated in a range of defined media varying in both the nature of the growth-limiting component and the concentration of excess nutrients. The compositions of the media were such as to ensure that the final absorbance ( ) of the culture was the same in each case. Samples taken during the stationary phase were assayed for their content of extracellular serine protease and bacitracin. The nature of the growth-limiting nutrient had a profound effect on the amounts of these products formed while those components which were present in excess also exerted an influence in proportion to their concentration. Thus, for example, a four-fold increase in serine protease production occurred when ammonium replaced glucose as the growth-limiting nutrient. Serine protease and bacitracin production responded differently to these varying cultural conditions suggesting they are subject to separate control mechanisms. The results are discussed in relation to the need for rigorously controlled cultural conditions during physiological studies of this nature.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-4-845
1982-04-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/4/mic-128-4-845.html?itemId=/content/journal/micro/10.1099/00221287-128-4-845&mimeType=html&fmt=ahah

References

  1. Dawes I. W., Mandelstam J. 1970; Speculation of Bacillus subtilis in continuous culture. Journal of Bacteriology 103:529–535
    [Google Scholar]
  2. Frøyshov Ø. 1974; Bacitracin biosynthesis by three complementary fractions from Bacillus licheniformis. FEBS Letters 44:75–78
    [Google Scholar]
  3. Grelet N. 1957; Growth limitation and spoliation. Journal of Applied Bacteriology 20:315–324
    [Google Scholar]
  4. Haavik H. I. 1974a; Studies on the formation of bacitracin by Bacillus licheniformis: effect of glucbse. Journal of General Microbiology 81:383–390
    [Google Scholar]
  5. Haavik H. I. 1974b; Studies on the formation of bacitracin by Bacillus licheniformis: role of catabolite repression and organic acids. Journal of General Microbiology 84:321–326
    [Google Scholar]
  6. Hanlon G. W., Hodges N. A. 1981a; Requirement for glucose during production of extracellular serine protease by cultures of Bacillus licheniformis. FEMS Microbiology Letters 11:51–54
    [Google Scholar]
  7. Hanlon G. W., Hodges N. A. 1981b; Bacitracin and protease production in relation to sporulation during exponential growth of Bacillus licheniformis on poorly utilized carbon and nitrogen sources. Journal of Bacteriology 147:427–431
    [Google Scholar]
  8. Hodges N. A., Brown M. R. W. 1975; Properties of Bacillus megaterium spores formed under conditions of nutrient limitation. In Spores VI pp. 550–555 Edited by Gerhardt P., Costilow R. N., Sadoff H. L. Washington, D.C.: American Society of Microbiology;
    [Google Scholar]
  9. Laishley E. J., Bernlohr R. W. 1968; Regulation of arginine, and proline catabolism in Bacillus licheniformis. Journal of Bacteriology 96:322–329
    [Google Scholar]
  10. Levisohn S., Aronson A. I. 1967; Regulation of extracellular protease production in Bacillus cereus. Journal of Bacteriology 93:1023–1030
    [Google Scholar]
  11. Martin J. F., Demain A. L. 1980; Control of antibiotic biosynthesis. Microbiological Reviews 44:230–251
    [Google Scholar]
  12. Priest F. G. 1977; Extracellular eilzyme synthesis in the genus Bacillus. Bacteriological Reviews 41:711–753
    [Google Scholar]
  13. Schaeffer P. 1967; Asporogeneous mutants of Bacillus subtilis Marburg. Folia microbiologica 12:291–296
    [Google Scholar]
  14. Vitković L., Sadoff H. L. 1975; Relationship between sporulation, protease and antibiotic in sporulating Bacillus licheniformis cells. In Spores VI pp. 362–366 Edited by Gerhardt P., Costilow R. N., Sadoff H. L. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  15. Vitković L., Sadoff H. L. 1977; In vitro production of bacitracin by proteolysis of vegetative Bacillus licheniformis cell protein. Journal of Bacteriology 131:897–905
    [Google Scholar]
  16. Wouters J. T. M., Buysman P. J. 1977; Production of some extracellular enzymes by Bacillus licheniformis 749/C in continuous cultures. FEMS Microbiology Letters 1:109–112
    [Google Scholar]
  17. Young M., Mandelstam J. 1980; Early events during bacterial endospore formation. Advances in Microbial Physiology 20:103–162
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-4-845
Loading
/content/journal/micro/10.1099/00221287-128-4-845
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error