1887

Abstract

Exogenous thymine was found to be taken up very slowly by in comparison to other pyrimidines, and most of it was catabolized by the cell. The existence of a functional, although inefficient, thymine salvage pathway was demonstrated and this pathway operated more effectively when thymidine nucleotide biosynthesis was inhibited by trimethoprim or methotrexate. The mechanism of thymine salvage by appears to be different from that of and as thymidine was not incorporated into the DNA. Like lacked thymidine phosphorylase activity. Unsuccessful attempts were made to isolate thymine auxotrophs.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-7-1391
1982-07-01
2024-05-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/7/mic-128-7-1391.html?itemId=/content/journal/micro/10.1099/00221287-128-7-1391&mimeType=html&fmt=ahah

References

  1. Ahmad S. I., Pritchard R. H. 1969; A map of four genes specifying enzymes involved in catabolism of nucleosides and deoxyribonucleosides in Escherichia coli . Molecular and General Genetics 104:351–359
    [Google Scholar]
  2. Boyce R. P., Setlow R. B. 1962; A simple method of increasing the incorporation of thymidine into the deoxyribonucleic acid of Escherichia coli . Biochimica et hiophvsica acta 61:618–620
    [Google Scholar]
  3. Breitman T. R., Bradford R. M. 1964; The induction of thymidine phosphorylase and excretion of deoxyribose during thymine starvation. Bio chemical and Biophysical Research Communica tions 17:786–791
    [Google Scholar]
  4. Budman D. R., Pardee A. B. 1967; Thymidine and thymine incorporation into deoxyribonucleic acid: inhibition and repression by uridine of thymidine phosphorylase of Escherichia coli . Journal of Bacteriology 94:1546–1550
    [Google Scholar]
  5. Bushby S. R. 1973; Sensitivity testing with trimethoprim/sulphamethoxazole. Journal of the Australian Medical Association, Wellcome Symposia 1:10–18
    [Google Scholar]
  6. Carmody J. M., Herriott R. M. 1970; Thymine and thymidine uptake by Haemophilus influenzae and the labelling of deoxyribonucleic acid. Journal of Bacteriology 101:525–530
    [Google Scholar]
  7. Carrier W. L., Setlow R. B. 1971; The excision of pyrimidine dimers. Methods in Enzymology 21:230–244
    [Google Scholar]
  8. Cauthen S. E., Foster M. A., Woods D. D. 1966; Methionine biosynthesis by extracts of Salmonella typhimurium . Biochemical Journal 48:630–639
    [Google Scholar]
  9. Davis B. D., Mingioli E. S. 1950; Mutants of E. coli requiring methionine and vitamin B12. Journal of Bacteriology 60:17–28
    [Google Scholar]
  10. Denhardt D. T. 1969; Formation of ribosyl-thymine in Escherichia coli . Journal of Biological Chemistry 244:2710–2715
    [Google Scholar]
  11. Friedkin M. 1973; Thymidylate synthetase. Advances in Enzymology 38:235–292
    [Google Scholar]
  12. Hatch F. T., Larrabee A. R., Cathou R., Buchanan J. M. 1961; Enzymatic synthesis of the methyl groups of methionine. I. Identification of the enzyme and co-factors involved in the system isolated from Escherichia coli . Journal of Biological Chemistry 236:1095–1108
    [Google Scholar]
  13. Holloway B. W. 1975; Genetic organisation of Pseudomonas. . In Genetics and Biochemistry of Pseudomonas pp. 133–162 Clarke P. H., Richmond M. H. Edited by New York: John Wiley;
    [Google Scholar]
  14. Isaac J. H., Holloway B. W. 1968; Control of pyrimidine biosynthesis in Pseudomonas aeruginosa . Journal of Bacteriology 46:1732–1741
    [Google Scholar]
  15. Kammen H. O. 1967; Thymine metabolism in Escherichia coli. 1. Factors involved in utilization of exogenous thymine. Biochimica et biophysica acta 134:301–311
    [Google Scholar]
  16. Kelln R. A., Warren R. A. J. 1973; Obligate thymidine auxotrophs of Pseudomonas acidovorans . Journal of Bacteriology 113:510–511
    [Google Scholar]
  17. Kelln R. A., Warren R. A. J. 1974; Pyrimidine metabolism in Pseudomonas acidovorans . Canadian Journal of Microbiology 20:427–433
    [Google Scholar]
  18. Kornberg A. 1974 DNA synthesis, 1st edn.. pp. 29–54 San Francisco: W. H. Freeman;
    [Google Scholar]
  19. Loutit J. S. 1969; Investigation of the mating system of Pseudomonas aeruginosa strain 1. V. The effect of N-methyl--nitro-N-nitrosoguanidine on a donor strain. Genetical Research 14:103–109
    [Google Scholar]
  20. Marmur J. 1961; A procedure for the isolation of DNA from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  21. Neuhard J., Price A. R., Schack L., Thomassen E. 1978; Two thymidylate synthetases in Bacillus subtilis . Proceedings of the National Academy of Sciences of the United States of America 75:1194–1198
    [Google Scholar]
  22. O’Donovan G. A., Neuhard J. 1970; Pyrimidine metabolism in microorganisms. Bacteriological Reviews 34:278–343
    [Google Scholar]
  23. Pemberton J. M., Clark A. J. 1973; Detection and characterization of plasmids in Pseudomonas aeruginosa strain PAO. Journal of Bacteriology 114:424–433
    [Google Scholar]
  24. Sakai T., Yu T., Omata S. 1976; Distribution of enzymes related to cytidine degradation in bacteria. Agricultural and Biological Chemistry 40:1893–1895
    [Google Scholar]
  25. Seydel J. K., Wempe E., Miller G. H., Miller L. 1972; Kinetics and mechanisms of action of trimethoprim and sulphonamides, alone or in com bination, upon Escherichia coli . Chemotherapy 17:217–238
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-7-1391
Loading
/content/journal/micro/10.1099/00221287-128-7-1391
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error