1887

Abstract

A plasmid (pSV1) of 110 × 10 daltons from a methylenomycin A producing strain of was detected on and isolated from agarose gels. Elimination of this plasmid by protoplasting and regeneration resulted in the simultaneous loss of methylenomycin A production and resistance. pSV1 hybridized with pBR322 containing a cloned fragment of 1.7 × 10 daltons from A3(2) which codes for methylenomycin A resistance. The pSV1 plasmid could be transferred to by conjugation and by transformation and plasmid DNA identical in size to pSV1 could be isolated from the recipient strains. These experiments show that pSV1 codes for methylenomycin A production and resistance, in close analogy to the SCP1 plasmid from A3(2).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-128-8-1893
1982-08-01
2024-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/128/8/mic-128-8-1893.html?itemId=/content/journal/micro/10.1099/00221287-128-8-1893&mimeType=html&fmt=ahah

References

  1. Aguilar A., Hopwood D. A. 1981; Isolation and characterization of a plasmid from Streptomyces violaceus-ruber and its relation to methylenomycin production. Society for General Microbiology Quarterly 8:135–136
    [Google Scholar]
  2. Bibb M. J., Freeman R. F., Hopwood D. A. 1977; Physical and genetical characterization of a second sex factor, SCP2, for Streptomyces coelicolor A3(2). Molecular and General Genetics 154:155–166
    [Google Scholar]
  3. Bibb M. J., Ward J. M., Hopwood D. A. 1978; Transformation of plasmid DNA into Streptomyces at high frequency. Nature; London: 274398–400
    [Google Scholar]
  4. Bibb M. J., Schottel J. L., Cohen S. N. 1980; A DNA cloning system for interspecies gene transfer in antibiotic-producing Streptomyces. Nature; London: 284526–531
    [Google Scholar]
  5. Bibb M. J., Ward J. M., Kieser T., Cohen S. N., Hopwood D. A. 1981; Excision of chromosomal DNA sequences from Streptomyces coelicolor forms a novel family of plasmids detectable in Streptomyces lividans. Molecular and General Genetics 184:230–240
    [Google Scholar]
  6. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  7. Chater K. F., Hopwood D. A., Kieser T., Thompson C. J. 1982; Gene cloning in Streptomyces. Current Topics in Microbiology and Immunology 96:69–95
    [Google Scholar]
  8. Cohen S. N., Chang A. C. Y., Hsu L. 1972; Nonchromosomal antibiotic resistance in bacteria: genetic transformation of E. coli by R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America 692110–2114
    [Google Scholar]
  9. Girvitz S. C., Bacchetti S., Rainbow A. J., Graham F. L. 1980; A rapid and efficient procedure for the purification of DNA from agarose gels. Analytical Biochemistry 106:492–496
    [Google Scholar]
  10. Haneishi T., Kitahara N., Takiguchi Y., Arai M., Sugawara S. 1974a; New antibiotics, methylenomycins A and B. I. Producing organism, fermentation and isolation, biological activities and physical and chemical properties. Journal of Antibiotics 27:386–392
    [Google Scholar]
  11. Haneishi T., Terahara A., Arai M., Hata T., Tamura C. 1974b; New antibiotics, methylenomycins A and B. II. Structure of methylenomycins A and B. Journal of Antibiotics 27:393–399
    [Google Scholar]
  12. Hombrecher G., Brewin N. J., Johnston A. W. B. 1981; Linkage of genes for nitrogenase and nodula- tion ability on plasmids in Rhizobium leguminosarum and R. phaseoli. Molecular and General Genetics 182:133–136
    [Google Scholar]
  13. Hopwood D. A. 1967; Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriological Reviews 31:373–403
    [Google Scholar]
  14. Hopwood D. A. 1978; Extrachromosomally determined antibiotic production. Annual Review of Microbiology 32:373–392
    [Google Scholar]
  15. Hopwood D. A. 1979; Genetics of antibiotic production by actinomycetes. Journal of Natural Products 42:596–602
    [Google Scholar]
  16. Hopwood D. A. 1981; Uses of protoplasts in the genetic manipulation of streptomycetes. In Actinomycetes pp. 523–531 Schaal K. P., Pulverer G. Edited by Zentralblatt fur Bakteriologie Suppl. 11 New York & Stuttgart: Gustav Fischer;
    [Google Scholar]
  17. Hopwood D. A., Wright H. M. 1976a; Genetic studies on SCP1-prime strains of Streptomyces coelicolor A3(2). Journal of General Microbiology 95:107–120
    [Google Scholar]
  18. Hopwood D. A., Wright H. M. 1976b; Interactions of the plasmid SCP1 with the chromosome of Streptomyces coelicolor A3(2). In Second International Symposium on the Genetics of Industrial Microorganisms pp. 607–619 Macdonald K. D. Edited by London: Academic Press;
    [Google Scholar]
  19. Hopwood D. A., Chater K. F., Dowding J. E., Vivian A. 1973; Advances in Streptomyces coelicolor genetics. Bacteriological Reviews 37:371–405
    [Google Scholar]
  20. Hopwood D. A., Bibb M. J., Ward J. M., Westpheling J. 1979; Plasmids in Streptomyces coelicolor and related species. In Plasmids of Medical, Environmental and Commercial Importance pp. 245–258 Timmis K. N., Puhler A. Edited by Amsterdam: Elsevier/North Holland;
    [Google Scholar]
  21. Hornemann U., Hopwood D. A. 1981; Biosynthesis of methylenomycin A, a plasmid determined antibiotic. In Antibiotics IV. Biosynthesis pp. 123–131 Corcoran J. W. Edited by Berlin: Spring-er-Verlag;
    [Google Scholar]
  22. Kirby R., Hopwood D. A. 1977; Genetic determination of methylenomycin synthesis by the SCP1 plasmid of Streptomyces coelicolor A3(2). Journal of General Microbiology 98:239–252
    [Google Scholar]
  23. Komatsu K., Leboul J., Harford S., Davies J. 1981; Studies of plasmids in neomycin-producing Streptomyces. In Microbiology 1981 pp. 384–387 Schlessinger D. Edited by Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  24. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  25. Novick R., Sanchez-Rivas C., Gruss A., Edelman I. 1980; Involvement of the cell envelope in plasmid maintenance: plasmid curing during the regeneration of protoplasts. Plasmid 3:348–358
    [Google Scholar]
  26. Okanishi M. 1979; Plasmids and antibiotic synthesis in streptomycetes. In Genetics of Industrial Microorganisms pp. 134–140 Sebek O. K., Laskin A. I. Edited by Washington D.C.: American Society for Microbiology;
    [Google Scholar]
  27. Okanishi M., Manome T., Umezawa H. 1980; Isolation and characterisation of plasmid DNAs in actinomycetes. Journal of Antibiotics 33:88–91
    [Google Scholar]
  28. Schrempf H., Goebel W. 1979; Functions of plasmid genes in Streptomyces reticuli. In Plasmids of Medical, Environmental and Commercial Importance pp. 259–268 Timmis K. N., Puhler A. Edited by Amsterdam: Elsevier/North Holland;
    [Google Scholar]
  29. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  30. Thompson C. J., Ward J. M., Hopwood D. A. 1982; Cloning of antibiotic resistance and nutritional genes in Streptomyces. Journal of Bacteriology in the Press
    [Google Scholar]
  31. Timmis K. F., Cabello F., Cohen S. N. 1978; Cloning and characterization of EcoRI and HindIII restriction endonuclease-generated fragments of antibiotic resistance plasmids R6-5 and R6. Molecular and General Genetics 162:121–137
    [Google Scholar]
  32. Vivian A. 1971; Genetic control of fertility in Streptomyces coelicolor A3(2): plasmid involvement in the interconversion of UF and IF strains. Journal of General Microbiology 69:353–364
    [Google Scholar]
  33. Wahl G. M., Stern M., Stark G. R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proceedings of the National Academy of Sciences of the United States of America 763683–3687
    [Google Scholar]
  34. Westpheling J. 1980 Physical studies of Streptomyces plasmids Ph.D. thesis University of East Anglia, Norwich; U.K.:
    [Google Scholar]
  35. Williams W., Katz E. 1977; Development of a chemically defined medium for the synthesis of ac- tinomycin D by Streptomyces parvulus. Antimicrobial Agents and Chemotherapy 11:281–290
    [Google Scholar]
  36. Wright L. F., Hopwood D. A. 1976; Identification of the antibiotic determined by the SCP1 plasmid of Streptomyces coelicolor A3(2). Journal of General Microbiology 95:96–106
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-128-8-1893
Loading
/content/journal/micro/10.1099/00221287-128-8-1893
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error