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Volume 128, Issue 9

Polyuronide Biosynthesis by Cell-free Extracts of Free

Abstract

Cell-free extracts from contain enzymes that catalyse the synthesis of uridine diphosphate glucuronic acid (UDPGlcA) from UDPglucose and the incorporation of glucuronic acid from UDPGlcA into polymer(s). Two different polyuronide fractions isolated from the cell walls of this fungus were used as primers. Mucoran, a heteropolymer, was much more efficient than mucoric acid, which is largely a homopolymer of -glucuronic acid. The primer ability of native cell walls was comparable to that of mucoric acid. Most of the glucuronosyltransferase activity in the cell-free extract was found in a 20000 particulate fraction. Optimum pH for polyuronide synthesis was 7·0. Mn or Mg stimulated incorporation of GlcA. The products synthesized from mucoric acid and mucoran primers were different and yielded different disaccharides upon hydrolysis.

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© Society for General Microbiology 1982
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/content/journal/micro/10.1099/00221287-128-9-2023
1982-09-01
2024-05-04
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References

  1. Bartnicki-Garcia S. 1968; Cell wall chemistry, morphogenesis, and taxonomy of fungi. Annual Review of Microbiology 22:87–108
     [Google Scholar]
  2. Bartnicki-Garcia S., Lindberg B. 1972; Partial characterization of mucoran: the glucuronomannan component. Carbohydrate Research 23:75–85
     [Google Scholar]
  3. Bartnicki-Garcia S., Nickerson W. J. 1962; Induction of yeastlike development in Mucor by carbon dioxide. Journal of Bacteriology 84:829–840
     [Google Scholar]
  4. Bartnicki-Garcia S., Reyes E. 1968a; Chemical composition of sporangiophore walls of Mucor rouxii. Biochimica et biophysica acta 165:32–42
     [Google Scholar]
  5. Bartnicki-Garcia S., Reyes E. 1968b; Polyuronides in the cell walls of .Mucor rouxii. Biochimica et biophysica acta 170:54–62
     [Google Scholar]
  6. Datema R., Van Den Ende H., Wessels J. G. H. 1977; The hyphal wall of Mucor mucedo 1. Polyanionic polymers. European Journal of Biochemistry 80:611–619
     [Google Scholar]
  7. Dow J. W., Witte J. T., Bindel K. R., Villa V. D. 1981a; Substrate-dependent activation of uridine diphosphoglucose dehydrogenase from the dimorphic fungus Mucor rouxii. Experimental Mycology 5:60–68
     [Google Scholar]
  8. Dow J. W., Carreon R. R., Villa V. D. 1981b; Role of membranes of mycelial Mucor rouxii in synthesis and secretion of cell wall matrix polymers. Journal of Bacteriology 145:272–279
     [Google Scholar]
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Polyuronide Biosynthesis by Cell-free Extracts of Mucor rouxii
Microbiology 128, 2023 (1982); https://doi.org/10.1099/00221287-128-9-2023
/content/journal/micro/10.1099/00221287-128-9-2023
/content/journal/micro/10.1099/00221287-128-9-2023
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