1887

Abstract

Enrichments were established to examine the potential of Blelham Tarn profundal sediment to metabolize benzoate to CH and CO. Longadaptation times were required before benzoate-dependent CH production occurred, though both increased inoculum size and prior methanogenic adaption to aliphatic fatty acids reduced the adaptation time. Benzoate was metabolized according to the stoichiometry: 4CHCOOH + 18HO 15CH + 13CO. The optimum temperature for CH production from benzoate in the enrichments was 37°C irrespective of the enrichment temperature. Methanogenic benzoate degradation was associated with a particulatefloc in the enrichments and was tentatively identified as an important constituent of this floc by scanning electron microscopy. Anaerobic benzoate fermentation was observed after 4 h in undiluted sediment by the use of [C]benzoate, and the temperature optimum for C-labelled gas formation was 28°C. The CH: CO ratio indicated that methanogenic fermentation of benzoate was occurring CO became the main gaseous product from [C]benzoate when sulphate was added to sediment, and 20 m-molybdate reversed this effect. Methanogenesis was slightly inhibited by addition of 20 m-molybdate. Methanogenic benzoate fermentation in sediments was found to be inhibited by H.

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1983-01-01
2024-05-04
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References

  1. Abram J. W., Nedwell D. B. 1978; Hydrogen as a substrate for methanogenesis and sulphate reduction in anaerobic saltmarsh sediment. Archives of Microbiology@ 117:93–97
    [Google Scholar]
  2. Ansbaek J., Blackburn T. H. 1980; A method for the analysis of acetate turnover in a coastal marine sediment. Marine Ecology 5:253–264
    [Google Scholar]
  3. Badziong W., Thauer R. K., Zeikus J. G. 1978; Isolation and characterisation of a Desulfovibrio growing on hydrogen plus sulfate as the sole energy source. Archives of Microbiology 116:41–49
    [Google Scholar]
  4. Balba M. T., Evans W. C. 1977; The methanogenic fermentation of aromatic compounds. Biochemical Society Transactions 5:302
    [Google Scholar]
  5. Boone D. R., Bryant M. P. 1980; Propionatedegrading bacterium, Syntrophobacter wolinii sp. nov. gen. nov., from methanogenic ecosystems. Applied and Environmental Microbiology 40:626–632
    [Google Scholar]
  6. Buswell A. M., Muller H. F. 1952; Mechanism of methane formation. Industrial and Engineering Chemistry 44:550–552
    [Google Scholar]
  7. Clark F. M., Fina L. R. 1952; The anaerobic decomposition of benzoic acid during methane fermentation. Archives of Biochemistry and Biophysics 36:26–32
    [Google Scholar]
  8. Digeronimo M. J., Nikaido M., Alexander M. 1978; Most probable number techniques for the enumeration of aromatic degraders in natural environments. Microbial Ecology 4:263–266
    [Google Scholar]
  9. Evans W. C. 1969; Microbial transformations of aromatic compounds. In: Fermentation Advances pp. 649–687 Perlman D. Edited by New York: Academic Press;
    [Google Scholar]
  10. Evans W. C. 1977; Biochemistry of the bacterial catabolism of aromatic compounds in anaerobic environments. Nature; London: 27017–22
    [Google Scholar]
  11. Ferry J. G., Wolfe R. S. 1976; Anaerobic degradation of benzoate to methane by a microbial consortium. Archives of Microbiology 107:33–40
    [Google Scholar]
  12. Fina L. R., Fiskin A. M. 1960; Anaerobic decomposition of benzoate during methane fermentation. II. Fates of carbons one and seven. Archives of Biochemistry and Biophysics 91:163–165
    [Google Scholar]
  13. Harshbarger T. H. 1977 Introductory Statistics. A Decision Map, 2nd edn. London: Collier Macmillan;
    [Google Scholar]
  14. Healy J. B., Young L. Y. 1979; Anaerobic degradation of eleven aromatic compounds to methane. Applied and Environmental Microbiology 39:216–218
    [Google Scholar]
  15. Healy J. B., Young L. Y., Reinhard M. 1980; Methanogenic idecomposition of ferulic acid, a model lignin derivative. Applied and Environmental Microbiology 39:436–444
    [Google Scholar]
  16. Horridge G. A., Tamm S. L. 1969; Critical point drying for scanning electron microscopic study of ciliary motion. Science 163:817–819
    [Google Scholar]
  17. Hungate R. E. 1969; A roll tube method for the cultivation of strict anaerobes. Methods in Microbiology 3B:117–132
    [Google Scholar]
  18. Jones J. G. 1973; Use of a nonparametric test for the analysis of data obtained from preliminary surveys: a review. Journal of Applied Bacteriology 36:197–210
    [Google Scholar]
  19. Jones J. G. 1976; The microbiology and decomposition of seston in open water and experimental enclosures in a productive lake. Journal of Ecology 64:241–278
    [Google Scholar]
  20. Jones J. G., Simon B. M. 1980a; Variability in microbiological data from a stratified eutrophic lake. Journal of Applied Bacteriology 49:127–135
    [Google Scholar]
  21. Jones J. G., Simon B. M. 1980b; Decomposition processes in the profundal region of Blelham Tam and the Lund tubes. Journal of Ecology 68:493–512
    [Google Scholar]
  22. Jones J. G., Simon B. M., Gardener S. 1982; Factors affecting methanogenesis and associated anaerobic processes in the sediments of a stratified eutrophic lake. Journal of General Microbiology 128:1–11
    [Google Scholar]
  23. Kaiser J. P., Hanselmann K. W. 1982; Aromatic chemicals through anaerobic microbial conversion of lignin monomers. Experientia 38:167–176
    [Google Scholar]
  24. Laanbroek H. G. A., Pfennig N. 1981; Oxidation of short-chain fatty acids by sulfate-reducing bacteria in freshwater and in marine sediments. Archives of Microbiology 128:330–335
    [Google Scholar]
  25. Mcinerney M. J., Bryant M. P., Pfennig N. 1979; Anaerobic bacterium that degrades fatty acids in syntrophic association with methanogens. Archives of Microbiology 122:129–135
    [Google Scholar]
  26. Nottingham P. M., Hungate R. E. 1969; Methanogenic fermentation of benzoate. Journal of Bacteriology 98:1170–1172
    [Google Scholar]
  27. Oremland R. S., Taylor B. F. 1978; Sulfate reduction and methanogenesis in marine sediments. Geochimica et cosmochimia acta 43:209–214
    [Google Scholar]
  28. Postgate J. R. 1969; Media for sulphur bacteria: some amendments. Laboratory Practice 18:286
    [Google Scholar]
  29. Shlomi E. R., Lankhorst A., Prins R. A. 1978; Methanogenic fermentation of benzoate in an enrichment culture. Microbial Ecology 4:249–261
    [Google Scholar]
  30. Smith M. R., Mah R. A. 1980; Acetate as the sole carbon and energy source for growth of Methanosar- cina strain 227. Applied and Environmental Microbiology 39:993–999
    [Google Scholar]
  31. Smith R. L., Klug M. J. 1981; Electron donors utilised by sulfate-reducing bacteria in eutrophic lake sediments. Applied and Environmental Microbiology 42:116–121
    [Google Scholar]
  32. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology 43:159–271
    [Google Scholar]
  33. Strayer R. F., Tiedje J. M. 1978; Kinetic parameters of the conversion of methane precursors to methane in a hypereutrophic lake sediment. Applied and Environmental Microbiology 36:330–340
    [Google Scholar]
  34. Tarvin D., Buswell A. M. 1934; The methane fermentation of organic acids and carbohydrates. Journal of the American Chemical Society 56:1751–1755
    [Google Scholar]
  35. White J., Yeats A., Skipworth G. 1979 Tables for Statisticians Cheltenham: Stanley Thornes;
    [Google Scholar]
  36. Widdel F. 1980 Anaerober Abbau von Fettsauren und Benzoesaure durch neu isolierte Arten sulfat-reduzier- ender Bakterien Dissertation zur Erlangung des Doktorgrades der Georg-August-Universität zu Göttingen.
    [Google Scholar]
  37. Winfrey M. R., Zeikus J. G. 1977; Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments. Applied and Environmental Microbiology 33:275–281
    [Google Scholar]
  38. Winfrey M. R., Nelson D. R., Klevickis S. C., Zeikus J. G. 1977; Association of hydrogen metabolism with methanogens in Lake Mendota sediments. Applied and Environmental Microbiology 33:312–318
    [Google Scholar]
  39. Zehnder A. J. B., Huser B. A., Brock T. D., Wuhrmann K. 1980; Characterisation of acetate- decarboxylating, non-hydrogen-oxidising methane bacterium. Archives of Microbiology 124:1–11
    [Google Scholar]
  40. Zeikus J. G., Winfrey M. R. 1976; Temperature limitations of methanogenesis in aquatic sediments. Applied and Environmental Microbiology 31:94–107
    [Google Scholar]
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