1887

Abstract

Isoelectric focusing of extracts of carrot root and apple fruit parenchyma infected by each of three isolates of showed the presence of forms of endopolygalacturonase (PG) with isoelectric points (pI) of about 4·5 (a minor peak) and 8·3–8·8. One isolate gave the hitherto unreported peak of pI 8·8 in both tissues, whereas the other two isolates gave a peak of 8·3. The molecular weights of the PGs of pI 8·3–8·8 were estimated to be about 30000. The variation in pi probably reflects the variability of No evidence of specific enzymic adaptation to host tissues was shown by the ability of PGs to cause changes in permeability of carrot and apple parenchyma.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-10-3015
1983-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/10/mic-129-10-3015.html?itemId=/content/journal/micro/10.1099/00221287-129-10-3015&mimeType=html&fmt=ahah

References

  1. Brown W. 1915; Studies in the physiology of parasitism. I. The action of Botrytis cinerea . Annals of Botany 29:313–348
    [Google Scholar]
  2. Byrde R.J.W., Willetts H.J. 1977 The Brown Rot Fungi of Fruit: Their Biology and Control Oxford: Pergamon Press;
    [Google Scholar]
  3. Dingle J., Reid W.W., Solomons G.L. 1953; The enzymic degradation of pectin and other polysaccharides. II. Application of the ‘cup-plate’ assay to the estimation of enzymes. Journal of the Science of Food and Agriculture 4:149–155
    [Google Scholar]
  4. Drawert F., Krefft M. 1978; Characterisierung extrazellularer Proteine und Enzyme aus Pektinkul-turfiltraten von Botrytis cinerea . Phytochemistry 17:887–890
    [Google Scholar]
  5. Fielding A.H. 1981; Natural inhibitors of fungal polygalacturonases in infected fruit tissues. Journal of General Microbiology 123:377–381
    [Google Scholar]
  6. Garibaldi A., Bateman D.F. 1971; Pectic enzymes produced by Erwinia chrysanthemi and their effects on plant tissue. Physiological Plant Pathology 1:25–40
    [Google Scholar]
  7. Grindle M. 1979; Phenotypic differences between natural and induced variants of Botrytis cinerea. . Journal of General Microbiology 111:109–120
    [Google Scholar]
  8. Magro P., Di Lenna P., Marciano P., Pallavi-Cini C. 1980; Variability of polygalacturonase and protein isoelectric focusing patterns in Botrytis cinerea isolates. Journal of General Microbiology 120:105–109
    [Google Scholar]
  9. Nelson N. 1944; A photometric adaptation of the Somogyi method for the determination of glucose. Journal of Biological Chemistry 153:375–380
    [Google Scholar]
  10. Willetts H.J., Byrde R.J.W., Fielding A.H., Wong A.-L. 1977; The taxonomy of the brown rot fungi (Monilinia spp.) related to their extracellular cell wall-degrading enzymes. Journal of General Microbiology 103:77–83
    [Google Scholar]
  11. Zalewska-Sobczak J., Urbanek H. 1975; Purification of polygalacturonase produced by Botrytis cinerea E-200. Bulletin de I’Academie polonaise des sciences: serie des sciences biologiques 23:663–668
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-10-3015
Loading
/content/journal/micro/10.1099/00221287-129-10-3015
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error