1887

Abstract

Enterotoxigenic strains of (ETEC) may produce a heat-labile enterotoxin (LT), a heat-stable enterotoxin (ST) or both enterotoxins. Certain serogroups are represented more frequently than others in ETEC isolated from humans. The transfer of three plasmids encoding enterotoxin production (Ent) to 22 non-toxigenic strains of many different O:H serotypes was studied. The Ent plasmids encoded ST (TP276), or LT (TP277), or ST + LT (TP214), and all carried antibiotic-resistance determinants. Twenty-one recipient strains acquired TP214, 18 acquired TP277 and 14 acquired TP276. Strains of those serotypes to which ETEC in diarrhoeal studies commonly belong neither acquired nor maintained Ent plasmids with a higher frequency than strains of those serotypes to which ETEC rarely belong. The recipient strains, with one exception, all expressed ST, or LT, or ST and LT, when they had acquired the appropriate plasmid; a non-motile strain belonging to O serogroup 88 expressed LT but failed to express ST when it acquired TP214 or TP277.

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1983-10-01
2024-05-03
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References

  1. Anderson E.S., Threlfall E.J. 1974; The characterisation of plasmids in the enterobacteria. Journal of Hygiene 72:471–487
    [Google Scholar]
  2. Back E., Mollby R., Kaijser B. 1980; Relative importance, seasonal variation, O- and K-antigens of enterotoxigenic Escherichia coli: a three-and-a-half year review in Sweden. Journal of Infection 2:302–315
    [Google Scholar]
  3. Cravioto A., Gross R.J., Scotland S.M., Rowe B. 1979; Mannose-resistant haemagglutination of human erythrocytes by strains of Escherichia colifrom extraintestinal sources: lack of correlation with colonisation factor antigen (CFA/I). FEMS Microbiology Letters 6:41–44
    [Google Scholar]
  4. Deboy J.M., Wachsmuth I.K., Davis B.R. 1980; Serotypes of enterotoxigenic Escherichia coliisolated in the United States. Infection and Immunity 29:361–368
    [Google Scholar]
  5. Evans D.G., Evans D.J. 1978; New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenic Escherichia coli of serogroups O6 and O8. Infection and Immunity 21:638–647
    [Google Scholar]
  6. Evans D.G., Silver R.P., Evans D.J., Chase D.G., Gorbach S.L. 1975; Plasmid-controlled colonization factor associated with virulence in Escherichia coli enterotoxigenic for humans. Infection and Immunity 12:656–667
    [Google Scholar]
  7. Evans D.G., Evans D.J., Tjoa W. 1977a; Hemagglutination of human group A erythrocytes by enterotoxigenic Escherichia coli isolated from adults with diarrhea: correlation with colonization factor. Infection and Immunity 18:330–337
    [Google Scholar]
  8. Evans D.J., Evans D.G., Dupont H.L., Ørskov F., ØRskov I. 1977b; Patterns of loss of enterotoxigenicity by Escherichia coli isolated from adults with diarrhea: suggestive evidence for an interrelationship with serotype. Infection and Immunity 17:105–111
    [Google Scholar]
  9. Foster T.J., Howe T.G.B., Richmond K.M.V. 1975; Translocation of the tetracycline resistance determinant from R100-1 to the Escherichia colichromosome. Journal of Bacteriology 124:1153–1158
    [Google Scholar]
  10. Fredericq P. 1957; Colicins. Annual Review of Microbiology 11:7–22
    [Google Scholar]
  11. Gross R.J., Scotland S.M., Rowe B. 1976; Enterotoxin testing of Escherichia coli causing epidemic infantile enteritis in the U.K. Lancet i:629–631
    [Google Scholar]
  12. Gyles C., So M., Falkow S. 1974; The enterotoxin plasmids of Escherichia coli. . Journal of Infectious Diseases 130:40–49
    [Google Scholar]
  13. Hoekstra W.P.M., Havekes A.M. 1979; On the role of the recipient cell during conjugation in Escherichia coli . Antonie van Leeuwenhoek 45:13–18
    [Google Scholar]
  14. Klipstein F.A., Rowe B., Engert R.F., Short H.B., Gross R.J. 1978; Enterotoxigenicity of enteropathogenic serotypes of Escherichia coli isolated from infants with epidemic diarrhea. Infection and Immunity 21:171–178
    [Google Scholar]
  15. Mcconnell M.M., Willshaw G.A., Smith H.R., Scotland S.M., Rowe B. 1979; Transposition of ampicillin resistance to an enterotoxin plasmid in an Escherichia coli strain of human origin. Journal of Bacteriology 139:346–355
    [Google Scholar]
  16. Mcconnell M.M., Smith H.R., Willshaw G.A., Scotland S.M., Rowe B. 1980; Plasmids coding for heat-labile enterotoxin production isolated from Escherichia coli 078: comparison of properties. Journal of Bacteriology 143:158–167
    [Google Scholar]
  17. Mcconnell M.M., Smith H.R., Willshaw G.A., Field A.M., Rowe B. 1981; Plasmids coding for colonization factor antigen I and heat-stable enterotoxin production isolated from enterotoxigenic Escherichia coli: comparison of their properties. Infection and Immunity 32:927–936
    [Google Scholar]
  18. Merson M.H., ØRskov F., ØRskov I., Sack R.B., Huq I., Koster F.T. 1979; Relationship between enterotoxin production and serotype in enterotoxigenic Escherichia coli . Infection and Immunity 23:325–329
    [Google Scholar]
  19. Merson M.H., Rowe B., Black R.E., Huq I., Gross R.J., Eusof A. 1980; Use of antisera for identification of enterotoxigenic Escherichia coli. . Lancet ii:222–224
    [Google Scholar]
  20. Moseley S.L., Huq I., Alim A.R.M.A., So M., Samadpour-Motalebi M., Falkow S. 1980; Detection of enterotoxigenic Escherichia coli by DNA colony hybridization. Journal of Infectious Diseases 142:892–898
    [Google Scholar]
  21. ØRskov I., ØRskov F. 1977; Special 0:K:H serotypes among enterotoxigenic E. coli strains from diarrhea in adults and children. Occurrence of the CF (colonization factor) antigen and of hemagglu-tinating abilities. Medical Microbiology and Immunology 163:99–110
    [Google Scholar]
  22. ØRskov F., ØRskov I., Evans D.J., Sack R.B., Sack D.A., Wadstrom T. 1976; Special Escherichia coli serotypes among enterotoxigenic strains from diarrhoea in adults and children. Medical Microbiology and Immunology 162:73–80
    [Google Scholar]
  23. Peneranda M.E., Mann M.B., Evans D.G., Evans D.J. 1980; Transfer of an ST :LT :CFA/II plasmid into Escherichia coli K12 strain RR1 by cotransformation with PSC301 plasmid DNA. FEMS Microbiology Letters 8:251–254
    [Google Scholar]
  24. Reis M.H.L., Matos D.P., Decastro A.F.P., Toledo M.R.F., Trabulsi L.R. 1980; Relationship among enterotoxigenic phenotypes, serotypes and sources of strains in enterotoxigenic Escherichia coli . Infection and Immunity 28:24–27
    [Google Scholar]
  25. Rowe B. 1973; The role of Escherichia coli in the diarrhoea of adults. 6th International Symposium of the World Association of Veterinary Food Hygienists. Elsinore.
    [Google Scholar]
  26. Scotland S.M., Gross R.J., Rowe B. 1977; Serotype-related enterotoxigenicity in Escherichia coli O6.H16 and 0148.H28. Journal of Hygiene 79:395–403
    [Google Scholar]
  27. Scotland S.M., Gross R.J., Cheasty T., Rowe B. 1979; The occurrence of plasmids carrying genes for both enterotoxin production and drug resistance in Escherichia coli of human origin. Journal of Hygiene 83:531–538
    [Google Scholar]
  28. Scotland S.M., Day N.P., Cravioto A., Thomas L.V., Rowe B. 1981; Production of heat-labile or heat-stable enterotoxins by strains of Escherichia colibelonging to serogroups 044, 0114 and O128. Infection and Immunity 31:500–503
    [Google Scholar]
  29. Smith H.R., Scotland S.M., Rowe B. 1983; Plasmids coding for production of colonization factor antigen II and enterotoxin production in strains of Escherichia coli. . Infection and Immunity 40:1236–1239
    [Google Scholar]
  30. Smyth C.J., Kaijser B., Back E., Faris A., Mollby R., Soderlind O., Stintzing G., Wadstrom T. 1979; Occurrence of adhesins causing mannose-resistant haemagglutination of bovine erythrocytes in enterotoxigenic Escherichia coli. . FEMS Microbiology Letters 5:85–90
    [Google Scholar]
  31. Taylor J. 1961; Host specificity and enteropatho-genicity of Escherichia coli. . Journal of Applied Bacteriology 24:316–325
    [Google Scholar]
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