1887

Abstract

Cerulenin and dodecanoic acid prevented the synthesis and secretion of glucosyltransferase in non-proliferating cell suspensions of ATCC 25975 under conditions that also inhibited the incorporation of radioactively labelled acetate into the cell. In the presence of Tween 80, acetate incorporation was not markedly affected by cerulenin despite the fact that glucosyltransferase secretion was still inhibited. Cerulenin and dodecanoic acid were found to prevent the incorporation of radioactively labelled acetate by affecting the uptake of acetate by the cell. In the case of cerulenin, the inhibition of uptake of acetate by the cell was partially relieved by the addition of Tween 80. These and other observations strongly suggested that cerulenin inhibited glucosyltransferase secretion and acetate incorporation by perturbing the membrane, rather than by directly inhibiting lipid synthesis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-11-3293
1983-11-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/11/mic-129-11-3293.html?itemId=/content/journal/micro/10.1099/00221287-129-11-3293&mimeType=html&fmt=ahah

References

  1. Bligh E.G., Dyer W.J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  2. Buttke T.M., Ingram L.O. 1980; Ethanol-induced changes in lipid composition of Escherichia coli: inhibition of saturated fatty acid synthesis in vitro. Archives of Biochemistry and Biophysics 203:565–571
    [Google Scholar]
  3. Carlsson J., Elander B. 1973; Regulation of dextransucrase formation by Streptococcus sanguis. Caries Research 7:81–101
    [Google Scholar]
  4. Caufield M.P., Berkeley R.C.W., Pepper E.A., Melling J. 1979; Export of extracellular levan- sucrase by Bacillus subtilis: inhibition by cerulenin and quinacrine. Journal of Bacteriology 138:345–351
    [Google Scholar]
  5. D’Agnolo G., Rosenfeld I.S., Awaya J., Omura S., Vagelos P.R. 1973; Inhibition of fatty acid synthesis by the antibiotic cerulenin. Biochimica et biophysica acta 326:155–166
    [Google Scholar]
  6. Hamilton I.R., Phipps P.J., Ellwood D.C. 1979; Effect of growth rate and glucose concentration on the biochemical properties of Streptococcus mutansIngbritt in continuous culture. In fection and Immunity 26:861–869
    [Google Scholar]
  7. Hardy L., Jacques N.A., Forester H., Campbell L.K., Knox K.W., Wicken A.J. 1981; Effect of fructose and other carbohydrates on the surface properties, lipoteichoic acid production and extracellular proteins of Streptococcus mutans Ingbritt grown in continuous culture. Infection and Immunity 31:78–87
    [Google Scholar]
  8. Hare M.D., Svensson S., Walker G.J. 1978; Characterization of the extracellular, water-insoluble α-d-glucans of oral streptococci by methylation analysis, and by enzymatic synthesis and degradation. Carbohydrate Research 66:245–264
    [Google Scholar]
  9. Jacques N.A. 1982; Relationship between cyclopropanesynthetase and the formation of cyclopropane fatty acids by Proteus vulgaris grown under various respiratory conditions. Journal of General Microbiology 128:177–184
    [Google Scholar]
  10. Jacques N.A., Wittenberger C.L. 1981; Inactivation of cell-associated fructosyltransferase in Streptococcus salivarius. Journal of Bacteriology 148:912–918
    [Google Scholar]
  11. Jain M.K., Wu N.M. 1977; Effect of small molecules on the dipalmitoyllecithin liposomal bilayer. III. Phase transition in lipid bilayer. Journal of Membrane Biology 34:157–201
    [Google Scholar]
  12. Janda W.M., Kuramitsu H.K. 1976; Regulation of extracellular glucosyltransferase production and the relationship between extracellular and cell-associated activities in Streptococcus mutans. Infection and Immunity 14:191–202
    [Google Scholar]
  13. Kuramitsu H.K., Wondrack L. 1980; Requirements for fatty acid synthesis and a chelation- sensitive step in the production of glucosyltransferase by Streptococcus mutans. Infection and Immunity 27:107–112
    [Google Scholar]
  14. Leung W.-L.S., Harlander S.K., Schachtele C.F. 1980; Streptococcus mutans dextransucrase: effect of cerulenin on lipid synthesis and enzyme production. In fection and Immunity 28:846–852
    [Google Scholar]
  15. Omura S. 1976; The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis. Bacteriological Reviews 40:681–697
    [Google Scholar]
  16. Paton J.C., May B.K., Elliott W.H. 1980; Cerulenin inhibits production of extracellular proteins but not membrane proteins in Bacillus amyloliquefaciens. Journal of General Microbiology 118:179–187
    [Google Scholar]
  17. Petit-Glatron M.-F., Chambert R. 1981; Levansucraseof Bacillus subtilis: conclusive evidence that its production and export are unrelated to fatty acid synthesis but modulated by membranemodifying agents. European Journal of Biochemistry 119:603–611
    [Google Scholar]
  18. Rigomier D, Bohin J.-P., Lubociiinsky B. 1980; Effects of ethanol and methanol on lipid metabolism in Bacillus subtilis. Journal of General Microbiology 121:139–149
    [Google Scholar]
  19. Robrish S.A., Reid W., Krichevsky M.I. 1972; Distribution of enzymes forming polysaccharides from sucrose and the composition of extracellular polysaccharides synthesized by Streptococcus mutans. Applied Microbiology 24:184–190
    [Google Scholar]
  20. Schachtele C.F., Harlander S.K., Germaine G.R. 1976; Streptococcus mutansdextransucrase: availability of disaggregated enzyme after growth in a chemically defined medium. In fection and Immunity 13:1522–1524
    [Google Scholar]
  21. Smith W.P., Tai P.C., Davis B.D. 1979; Extracellular labelling of growing secreted polypeptide chains in Bacillus subtiliswith diazoiodosulfonic acid. Biochemistry 18:198–202
    [Google Scholar]
  22. Umesaki Y., Kawai Y., Mutai M. 1977; Effect of Tween 80 on glucosyltransferase production in Streptococcus mutans. Applied and Environmental Microbiology 34:115–119
    [Google Scholar]
  23. Vadeboncoeur C., Trahan L. 1982; Glucose transport in Streptococcus salivarius. Evidence for the presence of a distinct phosphoenolpyruvate: glucose phosphotransferase system which catalyses the phosphorylation of a-methyl-glucoside. Canadian Journal of Microbiology 28:190–199
    [Google Scholar]
  24. Vance D., Goldberg I., Mitsuhashi O., Bloch K., Omura S., Nomura S. 1972; Inhibition of fatty acid synthetases by the antibiotic cerulenin. Biochemical and Biophysical Research Communications 48:649–656
    [Google Scholar]
  25. Wickner W. 1979; The assembly of proteins into biological membranes: the membrane trigger hypothesis. Annual Review of Biochemistry 48:23–45
    [Google Scholar]
  26. Wittenberger C.L., Wolf A.C. 1979; Possible role of fatty acid synthesis in the secretion of glucosyltransferase. Journal of Dental Research 58A:337
    [Google Scholar]
  27. Wittenberger C.L., Beaman A.J., Lee L.N. 1978; Tween 80 effect on glucosyltransferase synthesis by Streptococcus salivarius. Journal of Bacteriology 133:231–239
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-11-3293
Loading
/content/journal/micro/10.1099/00221287-129-11-3293
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error