1887

Abstract

The uptake and retention of purine bases and of uracil requires both a protonmotive force and intracellular conversion to nucleotides. Inhibition of uptake by arsenate does not imply that ATP is required for the transport process because arsenate caused a rapid fall in the level of 5-phosphoribosyl 1-pyrophosphate. A mutation defective in high-affinity adenine transport has been identified and is designated . This mutation has been found to lie in the neighbourhood of . Competition experiments indicate that at least two other systems are used to transport guanine, xanthine and hypoxanthine.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-11-3505
1983-11-01
2024-05-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/11/mic-129-11-3505.html?itemId=/content/journal/micro/10.1099/00221287-129-11-3505&mimeType=html&fmt=ahah

References

  1. Bachmann B.J., Low K.B. 1980; Linkage map of Escherichia coli K-12. Microbiological Reviews 44:1–56
    [Google Scholar]
  2. Benson C.L., Hornick D.L., Gots J.S. 1980; Genetic separation of purine transport from phosphoribosyltransferase activity in Salmonella typhimurium. Journal of General Microbiology 121:357–364
    [Google Scholar]
  3. Burton K. 1977; Transport of adenine, hypoxanthine and uracil into Escherichia coli. Biochemical Journal 168:195–204
    [Google Scholar]
  4. Cartier P.H. 1980; Adenine uptake by isolated rat thymocytes. Journal of Biological Chemistry 255:4574–4582
    [Google Scholar]
  5. Chevallier M.R., Jund R., Lacroute F. 1975; Characterization of cytosine permeation in Saccharomyces cerevisiae. Journal of Bacteriology 122:629–641
    [Google Scholar]
  6. Cybulski R.L., Fry D.W., Goldman I.D. 1981; Adenosine stimulation of uphill adenine transport in LI 210 leukemia cells. Journal of Biological Chemistry 256:4455–4459
    [Google Scholar]
  7. Darlington A.J., Scazzochio C. 1967; Use of analogues and the substrate-sensitivity of mutants in analysis of purine uptake and breakdown in Aspergillus nidulans. Journal of Bacteriology 93:937–940
    [Google Scholar]
  8. Fast R., SkÖld O. 1977; Biochemical mechanism of uracil uptake regulation in Escherichia coli B. Journal of Biological Chemistry 252:7620–7624
    [Google Scholar]
  9. Glover S.W. 1970; Functional analysis of host-specificity mutants in Escherichia coli. Genetical Research 15:237–250
    [Google Scholar]
  10. Hochstadt J. 1974; The role of the membrane in the utilization of nucleic acid precursors. CRC Critical Reviews in Biochemistry 2:259–309
    [Google Scholar]
  11. Hochstadt-Ozer J., Stadtman E.R. 1971; The regulation of purine utilization in bacteria. Journal of Biological Chemistry 246:5312–5320
    [Google Scholar]
  12. Jochimsen B., Nygaard P., Vestergaard T. 1975; Location on the chromosome of Escherichia coli of genes governing purine metabolism. Molecular and General Genetics 143:85–91
    [Google Scholar]
  13. Kadner R.J., Winkler H.H. 1975; Energy coupling for methionine transport in Escherichia coli. Journal of Bacteriology 123:985–991
    [Google Scholar]
  14. Kocharian S.M., Livshits V.A., Sukhodolets V.V. 1975; Genetic study of Escherichia coli K-12 mutants resistant to 2,6-diaminopurine. Genetika 11:79–89
    [Google Scholar]
  15. Levine R.A., Taylor M.W. 1981; Selection for purine regulatory mutants in an E. coli hypoxanthine phosphoribosyltransferase-guanine phosphoribosyltransferase double mutant. Molecular and General Genetics 181:313–318
    [Google Scholar]
  16. Lierberman I., Kornberg A., Simms E.S. 1955; Enzymatic synthesis of pyrimidine nucleotides.Orotidine 5′-phosphate and uridine 5′-phosphate. Journal of Biological Chemistry 215:403–415
    [Google Scholar]
  17. Lind R.M., Sukhodolets V.V., Smirnov Y.V. 1973; Mutations disturbing cytosine deamination and transport in Escherichia coli. Genetika 9:116–121
    [Google Scholar]
  18. Low B. 1973; Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12. Journal of Bacteriology 113:798–812
    [Google Scholar]
  19. Miller J.H. 1972 Experiments in molecular genetics. New York:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  20. Miller R.L., Ramsey G.A., Krenitsky T.A., Elion G.B. 1972; Guanine phosphoribosyltransferase from Escherichia coli, specificity and properties. Biochemistry 11:4723–4731
    [Google Scholar]
  21. Molloy A., Finch L.R. 1969; Uridine 5′-monophosphate pyrophosphorylase activity from Escherichia coli. FEBS Letters 5:211–213
    [Google Scholar]
  22. Nygaard P. 1981; Adenine transport in Escherichia coli. Biochemical Society Transactions 9:179P
    [Google Scholar]
  23. Page M.G.P., Burton K. 1978; The location of purine phosphoribosyltransferase activities in Escherichia coli. Biochemical Journal 174:717–725
    [Google Scholar]
  24. Plagemann P.G.W., Wohlhueter R.M. 1980; Permeation of nucleosides, nucleic acid bases and nucleotides in animal cells. Current Topics in Membranes & Transport 14:225–330
    [Google Scholar]
  25. Polak A., Grenson M. 1973; Evidence for a common transport system for cytosine, adenine and hypoxanthine in Saccharomyces cerevisiae and Candida albans. European Journal of Biochemistry 32:276–282
    [Google Scholar]
  26. Reichert U., Winter M. 1974; Uptake and accumulation of purine bases by stationary yeast cells pretreated with glucose. Biochimica et biophysica acta 356:108–116
    [Google Scholar]
  27. Roy-Burman S., Visser D.W. 1975; Transport of purines and deoxyadenosine in Escherichia coli. Journal of Biological Chemistry 250:9270–9275
    [Google Scholar]
  28. Roy-Burman S., Von Dippe P., Visser D.W. 1978; Mechanism of energy coupling for transport of deoxycytidine, uridine, uracil, adenine and hypoxanthine in Escherichia coli. Biochimica et biophysica acta 511:285–296
    [Google Scholar]
  29. Schairer H.U., Haddock B.A. 1972; fl-Galactoside accumulation in a Mg2+-, Ca2 +-activated ATPase deficient mutant of E. coli. Biochemical and Biophysical Research Communications 48:544–571
    [Google Scholar]
  30. Schmidt R., ForÊt M., Reichert U. 1976; Improved microscale assay for purine phosphoribosyltransferase activities. Clinical Chemistry 22:67–69
    [Google Scholar]
  31. Sin I.L., Finch L.R. 1972; Adenine phosphoribosyltransferase in Mycoplasma mycoides and Escherichia coli. Journal of Bacteriology 112:439–444
    [Google Scholar]
  32. Templeton B., Chilson O.P. 1981; Adenine transport by mature rabbit erythrocytes. Journal of Biological Chemistry 256:285–290
    [Google Scholar]
  33. Thakar J.H., Kalle G.P. 1968; Defective guanine uptake in an 8-azaguanine-resistant mutant of Salmonella typhimurium. Journal of Bacteriology 95:458–464
    [Google Scholar]
  34. Tsao T.-F., Marzluf G.A. 1976; Genetic and metabolic regulation of purine-base transport in Neurospora crassa. Molecular and General Genetics 149:347–355
    [Google Scholar]
  35. Yajima N., Kondo K., Morita K. 1981; Reverse mutation tests in Salmonella typhimurium and chromosomal aberration tests in mammalian cells in culture on fluorinated pyrimidine derivatives. Mutation Research 88:241–254
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-11-3505
Loading
/content/journal/micro/10.1099/00221287-129-11-3505
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error