1887

Microbiology Society logo

Volume 129, Issue 5

Altered Colonizing Ability for Mouse Large Intestine of a Surface Mutant of a Human Faecal Isolate of Escherichia coli Free

Abstract

F-17 S a human faecal isolate, is resistant to the T-series of bacteriophages (i.e. T2 to T7). A T2-sensitive mutant of F-17 S was isolated following acriflavin treatment. This mutant, F-17 S T was found to be sensitive to the entire T-series of phages. F-17 S and F-17 S T did not differ quantitatively in total LPS content. However, analysis of LPS revealed that a large fraction of F-17 S T was devoid of O-side-chains. This accounted for the sensitivity of this strain to bacteriophages T3, T4, and T7. In addition, F-17 S T contained only about half the amount of capsular material contained by F-17 S accounting for the sensitivity of the mutant to bacteriophages T2, T5, and T6. Although the two strains colonized equally well when fed individually to streptomycin-treated mice, when fed simultaneously to streptomycin-treated mice, F-17 S T colonized at a level of about 1 × 10 cells (g faeces), whereas F-17 S colonized at only 1 × 10 cells (g faeces). These studies suggest that bacterial cell surface components modulate the large intestine colonizing ability of F-17 S in the mouse large intestine.

  • Received:
  • Revised:
  • Published Online:
© Society for General Microbiology, 1983
Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-5-1549
1983-05-01
2024-04-30
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/5/mic-129-5-1549.html?itemId=/content/journal/micro/10.1099/00221287-129-5-1549&mimeType=html&fmt=ahah

References

  1. Adams M. H. 1959 Bacteriophages pp. 467–468 New York: Interscience;
     [Google Scholar]
  2. Braun V., Hantke K. 1981; Bacterial cell surface receptors. In Organization of Prokaryotic Cell Membranes II pp. 1–52 Edited by Glosh B. K. Boca Raton: CRC Press;
     [Google Scholar]
  3. Bray J. 1945; Isolation of antigenically homogenous strains of Bacterium coli neapolitanum from summer diarrhea of infants. Journal of Pathology and Bacteriology 57:239–245
     [Google Scholar]
  4. Burrows M. R., Sellwood R., Gibbons R. G. 1976; Haemagglutinating and adhesive properties associated with the K99 antigen of bovine strains of Escherichia coli. Journal of General Microbiology 96:269–275
     [Google Scholar]
  5. Clancy J., Savage D. C. 1981; Another colicin V phenotype: In vitro adhesion of Escherichia coli to mouse intestinal epithelium. Infection and Immunity 32:343–352
     [Google Scholar]
  6. Dische Z. 1955; New color reactions for determination of sugars in polysaccharides. In Methods of Biochemical Analysis II pp. 313–358 Edited by Glick D. London: Interscience;
     [Google Scholar]
  7. Dufour A. P., Strickland E. R., Cabelli V. J. 1981; Membrane filter method for enumerating Escherichia coli. Applied and Environmental Microbiology 41:1152–1158
     [Google Scholar]
  8. Dupont H. L., Formal S. B., Hornick R. B., Snyder M. J., Libonati J. P., Sheahan D. G., Labrec E. H., Kalas J. P. 1971; Pathogenesis of E. coli diarrhea. New England Journal of Medicine 385:1–9
     [Google Scholar]
  9. Evans D. E., Silver R. P., Evans D. J., Chase D. G., Gorbach S. L. 1975; Plasmid-controlled colonization factor associated with virulence in Escherichia colienterotoxigenic for humans. Infection and Immunity 12:656–664
     [Google Scholar]
  10. EVANS D. G., Evans D. J.JR 1978; New surface- associated heat-labile colonization factor (CFA/II) produced by enterotoxigenicEscherichia coli of serogroups 06 and 08. Infection and Immunity 21:638–647
     [Google Scholar]
  11. Formal S. B., Dupont H. L., Hornick R., Snyder M. J., Libonati J., Labrec E. H. 1971; Experimental models in the investigation of the virulence of dysentery bacilli. Annals of the New York Academy of Sciences 176:190–202
     [Google Scholar]
  12. Goldman R. C., Leive L. 1980; Heterogeneity of antigenic-side-chain length in lipopolysaccharide from Escherichia coli 0111 and Salmonella typhimurium LT2. European Journal of Biochemistry 107:145–153
     [Google Scholar]
  13. Guerry P., Leblanc D. J., Falkow S. 1973; General method for the isolation of plasmid deoxyribonucleic acid. Journal of Bacteriology 116:1064–1070
     [Google Scholar]
  14. Gyles C., Falkow S., Rollins L. 1978; In vivo transfer of a plasmid possessing genes for Escherichia colienterotoxin. In Microbiology-1978 pp. 267–269 Edited by Schlessinger D. Washington D.C.: American Society for Microbiology;
     [Google Scholar]
  15. Hartley C. L., Newmann C. S., Richman M. H. 1979; Adhesion of commensal bacteria to the large intestine wall in humans. Infection and Immunity 23:128–132
     [Google Scholar]
  16. Havekes L. M., Lugtenberg B. J. J., Hoekstra P. M. 1976; Conjugation deficient E.coli K12F-mutants with hepatose-less lipopolysaccharide. Molecular and General Genetics 146:43–50
     [Google Scholar]
  17. IZHAR M., Nuchamowitz Y., Mirelman D. 1982; Adherence of Shigella flexneri to guinea-pig intestinal cells is mediated by a mucosal adhesin. Infection and Immunity 35:1110–1118
     [Google Scholar]
  18. Jann K., Jann B., Schmidt G., Blumenstock E., Vosbeck K. 1981; Adherence of Escherichia coli to mammalian and yeast cells.The role of piliation and surface hydrophobicity. Infection and Immunity 32:484–489
     [Google Scholar]
  19. Jentoft N., Dearborn D. G. 1979; Labeling of proteins by reductive methylation using sodium cyanoborohydride. Journal of Biological Chemistry 254:4359–4365
     [Google Scholar]
  20. Karkhanis Y. D., Zeltner J. Y., Jackson J. J., Carlo D. J. 1978; A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of gram-negative bacteria. Analytical Biochemistry 85:595–601
     [Google Scholar]
  21. Laskey R. A., Mills D. A. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
     [Google Scholar]
  22. Lindberg A. A. 1973; Bacteriophage receptors. Annual Review of Microbiology 27:205–241
     [Google Scholar]
  23. Lynch K. R., Pennica D., Ennis H.L, Cohen P. S. 1979; Separation and purification of the mRNAs for vesicular stomatitis virus NS and M proteins. Virology 98:251–254
     [Google Scholar]
  24. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. 1976; Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology 127:1529–1538
     [Google Scholar]
  25. Mitsuhashi S., Harada K., Kameda M. 1961; Elimination of transmissible drug-resistance by treatment with acriflavin. Nature, London 189:947
     [Google Scholar]
  26. Myhal M. L., Laux D. C., Cohen P. S. 1982; Relative colonizing abilities of human fecal and K12 strains of Escherichia coli in the large intestines of streptomycin-treated mice. European Journal of Clinical Microbiology 1:186–192
     [Google Scholar]
  27. Nagy B., Moon H. W., Isaacson R. E. 1977; Colonization of porcine intestine by enterotoxigenicEscherichia coli: selection of piliated forms in vivo, adhesion of piliated forms to epithelial cells in vitro, and incidence of a pilus antigen among porcine enteropathogenic E. coli. Infection and Immunity 16:344–352
     [Google Scholar]
  28. Nikaido H. 1973; Biosynthesis and assembly of lipopolysaccharide and the outer membrane layer of gram-negative cell wall. In Bacterial Membranes and Walls pp. 131–208 Edited by Leive L. New York: Marcel Dekker;
     [Google Scholar]
  29. Ørskov I., Ørskov F. 1966; Episome-mediated surface antigen K88 of Escherichia coli. I. Transmission of the determinant of the K88 antigen and influence on the transfer of chromosomal markers. Journal of Bacteriology 91:69–75
     [Google Scholar]
  30. Ørskov I., Ørskov F., Smith H. W., Sojka W. J. 1975; The establishment of K99, a thermolabile, transmissible Escherichia coli K antigen, previously called “Kco”, possessed by calf and lamb enteropathogenic strains. Actapathologia et microbiologicas candinavica(Microbiology and Immunology) 83:31–36
     [Google Scholar]
  31. Palva E. T., Makela P. H. 1980; Lipopolysaccharide heterogeneity in Salmonella typhimuriumanalyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. European Journal of Biochemistry 107:137–143
     [Google Scholar]
  32. Revel H. R. 1967; Restriction of nonglucosylated T-even bacteriophage: properties of permissive mutants of E. coli B and K12. Virology 31:688–701
     [Google Scholar]
  33. Schnaitman C. A. 1974; Outer membrane proteins of Escherichia coli. III. Evidence that the major protein of Escherichia coli 0111 outer membrane consists of four distinct polypeptide species. Journal of Bacteriology 118:443–453
     [Google Scholar]
  34. Smith H. W., Linggood M. A. 1971; Observations on the pathogenic properties of the K88, Hly and Ent plasmids of Escherichia coli with particular reference to porcine diarrhea. Journal of Medical Microbiology 4:467–485
     [Google Scholar]
  35. Smyth C. J., Jonsson P., Olsson E., Soderlind O., Rosengren J., Hjerten S., Wadstrom T. 1978; Difference in hydrophobic surface characteristics of porcine enteropathogenic Escherichia coli with or without K88 antigen as revealed by hydrophobic interaction chromatography. Infection and Immunity 22:462–472
     [Google Scholar]
  36. Taylor J., Powell B. W., Wright J. 1949; Infantile diarrhea and vomiting. British Journal of Medicine 2:117–125
     [Google Scholar]
  37. Tsai C. M., Frasch C. E. 1982; A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Analytical Biochemistry 119:115–119
     [Google Scholar]
  38. Westphal O., Jann K. 1965; Bacterial lipopolysaccharides.Extraction with phenol-water and further applications of the procedure. Methods in Carbohydrate Chemistry 5:83–91
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-5-1549
Loading
Altered Colonizing Ability for Mouse Large Intestine of a Surface Mutant of a Human Faecal Isolate of Escherichia coli
Microbiology 129, 1549 (1983); https://doi.org/10.1099/00221287-129-5-1549
/content/journal/micro/10.1099/00221287-129-5-1549
/content/journal/micro/10.1099/00221287-129-5-1549
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error