1887

Abstract

Several hybrid cell lines producing monoclonal antibodies against gonococcal pili have been derived. The reactivity of each antibody against variant pili of strain P9 was determined by ELISA. Using type-specific as well as broadly cross-reacting antibodies labelled with I, the capacity of unlabelled hybridoma antibodies to inhibit their attachment to various pilus types was determined. Some antibodies competed significantly and were judged to react at the same or closely positioned antigenic sites, whereas others bound non-competitively to the same pilus. These studies suggest the existence of distinct antigenic sites on gonococcal pili: a common antigenic region shared by all P9 pilus types and a type-specific region. Both antigenic sites contain more than one epitope.

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/content/journal/micro/10.1099/00221287-129-6-1965
1983-06-01
2024-04-27
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References

  1. Blake M. S., Swanson J. 1975; Studies on gonococcus infection. IX. In vitro decreased association of pilated gonococci with mouse peritoneal macrophages. Infection and Immunity 11:1402–1404
    [Google Scholar]
  2. Brinton C. C., Bryan J., Dillon J.-A., Guerina N., Jacobson L. J., Labik A., Lee S., Levine A., Lim S., Mcmichael J., Polen S., Rogers K., To A.C.-C., To S.C.-M. 1978; Uses of pili in gonorrhea control: role of bacterial pili in diseases, purification and properties of gonococcal pili, and progress in the development of a gonococcal pilus vaccine for gonorrhea. In Immunobiology of Neisseria gonorrhoeae pp. 155–178 Edited by Brooks G. F., Gotschlich E. C., Holmes K. K., Sawyer W. D., Young F. E. Washington, D. C.: American Society for Microbiology;
    [Google Scholar]
  3. Buchanan T. M. 1978; Antigen specific serotyping of Neisseria gonorrhoeae. I. Use of an enzyme-linked immunosorbent assay to quantitate pili antigens on gonococci. Journal of Infectious Diseases 138:319–325
    [Google Scholar]
  4. Buchanan T. M., Siegel M. S., Chen K. C. S., Pearce W. A. 1982; Development of a vaccine to prevent gonorrhoea. In Seminars in Infectious Disease. Vol. IV: Bacteria! Vaccines pp. 160–164 Edited by Weistein L., Fields B. N., Robbins J. B., Hill J. C., Sadoff G. New York: Thieme-Stratton;
    [Google Scholar]
  5. Dilworth J. A., Hendley J. O., Mandell G. L. 1975; Attachment and ingestion of gonoccoci by human neutrophils. Injection and Immunity 11:512–516
    [Google Scholar]
  6. Fazekas De Stgroth S., Scheidegger D. 1980; Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35:1–21
    [Google Scholar]
  7. Heckels J. E. 1981; Structural comparison of Neisseria gonorrhoeae outer membrane proteins. Journal of Bacteriology 145:736–742
    [Google Scholar]
  8. Heckels J. E. 1982; The role of surface proteins in the adhesion of Neisseria gonorrhoeae. . In Microbiology 1982 Edited by Schlessinger D. Washington, D. C.: American Society for Microbiology;
    [Google Scholar]
  9. Jones R. B., Newland J. C., Olsen D. A., Buchanan T. M. 1980; Immune-enhanced phagocytosis of Neisseria gonorrhoeae by macrophages: characterization of the major antigens to which opsonins are directed. Journal of General Microbiology 121:365–372
    [Google Scholar]
  10. Lambden P. R. 1982; Biochemical comparison of pili from variants of Neisseria gonorrhoeae P9. Journal of General Microbiology 128:2105–2111
    [Google Scholar]
  11. Lambden P. R., Heckels J. E. 1982; Synthesis of immunogenic oligosaccharide-protein conjugates from the lipopolysaccharide of Neisseria gonorrhoeae P9. Journal of Immunological Methods 48:233–240
    [Google Scholar]
  12. Lambden P. R., Heckels J. E., Mcbride H., Watt P. J. 1981; The identification and isolation of novel pilus types produced by variants of Neisseria gonorrhoeae P9 following selection in vivo. . FEMS Microbiology Letters 10:339–341
    [Google Scholar]
  13. Mårdh P. A., Weström L. 1976; Adherence of bacteria to vaginal epithelial cells. Injection and Immunity 13:661–666
    [Google Scholar]
  14. Potter M., Humphrey J. G., Wallers J. L. 1972; Growth of primary plasmacytomas in the mineral oil-conditioned peritoneal environment. Journal of the National Cancer Institute 72:305–308
    [Google Scholar]
  15. Schoolnik G. K., Tai J.-Y., Gotschlich F. C. 1982; The human erythrocyte binding domain of gonococcal pili. In Seminars in Infectious Disease. Vol. IV: Bacterial Vaccines pp. 172–180 Edited by Weinstein L., Fields B. N., Robbins J. B., Hill J. C., Sadoff G. New York: Thieme-Stratton;
    [Google Scholar]
  16. Stone M. R., Nowinski R. C. 1980; Topological mapping of murine leukaemia virus proteins by competition binding assays with monoclonal antibodies. Virology 100:370–381
    [Google Scholar]
  17. Towbin H., Stachelin T., Gordon J. 1979; Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  18. Tramont E. C. 1976; Specificity of inhibition of epithelial cell adhesion of Neisseria gonorrhoeae. . Infection and Immunity 14:593–595
    [Google Scholar]
  19. Virji M. 1981; The effect of antibody directed against outer membrane antigens on the virulence of a pilated variant of Neisseria gonorrhoeae. . FEMS Microbiology Letters 12:355–358
    [Google Scholar]
  20. Virji M., Everson J. S., Lambden P. R. 1982; Effect of anti-plus antisera on virulence of variants of Neisseria gonorrhoeae for cultured epithelial cells. Journal of General Microbiology 128:1095–1100
    [Google Scholar]
  21. Watanabe M., Ishii T., Nariuchi H. 1981; Fractionation of IgG,, IgG2a, IgG2b and IgG3 immunoglobulins from mouse serum by protein A- Sepharose column chromatography. Japanese Journal of Experimental Medicine 51:65–70
    [Google Scholar]
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