1887

Abstract

A total of 132 moderately halophilic bacteria were isolated from hypersaline soils with a Cl content between 2.36 and 12.72% (w/v) located near Alicante (S.E. Spain) and examined for 98 phenotypic characteristics including their response to cytological, physiological, biochemical and nutritional tests. They were submitted to a numerical analysis together with six reference strains using both simple matching () and Jaccard () coefficients, and cluster analysis was carried out by the unweighted pair group method of association (UPGMA), single linkage and complete linkage. With the coefficient and UPGMA clustering, eight phenons were obtained at the 65% similarity level. From each phenon representative strains were chosen for the determination of DNA base composition and for electron microscopy. Bacteria belonging to phenons D, E, and F were assigned to the genus Phenon G included 27 strains assigned to , but the high G + C composition (58.9 mol %) of a representative strain of this phenon suggests that it may represent a new taxon. Phenons A, B, and C were designated and phenon H was The bacteria found in these environments are not related to those from hypersaline waters or normal soils.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-129-8-2649
1983-08-01
2024-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/129/8/mic-129-8-2649.html?itemId=/content/journal/micro/10.1099/00221287-129-8-2649&mimeType=html&fmt=ahah

References

  1. Alexander M. 1961 Soil Microbiology. Edited by Wiley J. New York: Blackwell;
    [Google Scholar]
  2. Baumann L., Baumann P., Mandel M., Allen R.D. 1972; Taxonomy of aerobic marine eubacteria. Journal of Bacteriology 110:402–429
    [Google Scholar]
  3. Buchanan R.E., Gibbons N.E. editor 1974 Bergey’s Manual of Determinative Bacteriology, 8th edn.. Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Cervantes L.A., Olivares J. 1976; Estudiomicrobiologico de unsuelosalino “El Salitre”. Revista latinoamericana de microbiologia 18:73–76
    [Google Scholar]
  5. Elazari-Volcani B. 1940 Studies on the microflora of the Dead Sea. Ph.D. thesis Hebrew University, Jerusalem:
    [Google Scholar]
  6. Hayes P.R., Mapp S.J. 1981; Taxonomic studies on high G + C % flavobacteria. In The Flavobacteria-CytophagaGroup pp. 33–38 Edited by Reichenbach M., Weeks O. B. Weinheim: Verlag Chemie;
    [Google Scholar]
  7. Henis Y., Eren J. 1963; Preliminary studies on the microflora of a highly saline soil. Canadian Journal of Microbiology 9:903–904
    [Google Scholar]
  8. Henriksen S.D. 1973; Moraxella, Acinetobacter and the Mimeae. Bacteriological Reviews 37:522–561
    [Google Scholar]
  9. Holmes B., Owen R.J. 1979; Proposal that Flavo-bacterium breve be substituted as the type species of the genus in place of Flavobacteriumaquatile and emended description of the genus Flavobacterium:status of the named species of Flavobacterium. International Journal of Systematic Bacteriology 29:4l6–426
    [Google Scholar]
  10. Holmes B., Owen R.J. 1981; Emendation of the genus Flavobacterium and the status of the genus. Development after the 8th edition of Bergey’s manual. In The Flavobacterium-Cytophaga Group. Edited by Reichenbach M., Weeks O. B. Weinheim: Verlag Chemie;
    [Google Scholar]
  11. Jaccard P. 1908; Nouvellesrecherchessur la distribution florale. Bulletin de la Société vaudoise des sciences naturelles 44:223–270
    [Google Scholar]
  12. Kushner D.J. 1978; Life in high salt and solute concentrations: halophilic bacteria. In Microbial Life in Extreme Environments pp. 317–368 Edited by Kushner D. J. London: Academic Press;
    [Google Scholar]
  13. Larsen H. 1962; Halophilism. In The Bacteria IV pp. 297–336 Edited by Gunsalus I. C., Stanier R. Y. London: Academic Press;
    [Google Scholar]
  14. Mahmoud S.A.Z., Elsawy M., Ishac Y.L., Elsafty M.M. 1978; The effects of salinity and alkalinity on the distribution and capacity of N2- fixation by Azotobacter in Egyptian soils. Environmental role of nitrogen-fixing blue-green algae and symbiotic bacteria. Ecological Bulletin 26:99–109
    [Google Scholar]
  15. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  16. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  17. Mcmeekin T.A., Shewan J.M. 1978; Taxonomic strategies for Flavobacterium and related genera. Journal of Applied Bacteriology 45:321–332
    [Google Scholar]
  18. Novitsky T.J., Kushner D.J. 1976; Planococcushalophilus sp. nov., a facultativelyhalophiliccoccus. International Journal of Systematic Bacteriology 26:53–57
    [Google Scholar]
  19. Onishi H., Hidaka O. 1978; Purification and properties of amylase produced by a moderately halophilicAcinetobactersp. Canadian Journal of Microbiology 24:1017–1023
    [Google Scholar]
  20. Quesada E., Ventosa A., Rodriguez-Valera F., Ramos-Cormenzana A. 1982; Types and properties of some bacteria isolated from hypersaline soils. Journal of Applied Bacteriology 53:155–161
    [Google Scholar]
  21. Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1980; Isolation of extremely halophilic bacteria able to grow in defined inorganic media with single carbon sources. Journal of General Microbiology 119:535–538
    [Google Scholar]
  22. Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1981; Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations. Microbial Ecology 1:235–243
    [Google Scholar]
  23. Skerman V.B.D., Mcgowan V., Sneath P.H.A. 1980; Approved lists of bacterial names. International Journal of Systematic Bacteriology 30:225–420
    [Google Scholar]
  24. Sneath P.H.A., Johnson R. 1972; The influence on numerical taxonomic similarities of errors in microbiological tests. Journal of General Microbiology 72:373–392
    [Google Scholar]
  25. Sneath P.H.A., Sokal R.R. 1973 Numerical Taxonomy. The Principles and Practice of Numerical Classification. San Francisco: W. H. Freeman;
    [Google Scholar]
  26. Sokal R. R., Michener C.D. 1958; A statistical method for evaluating systematic relationships. University of Kansas Science Bulletin 38:1409–1438
    [Google Scholar]
  27. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. Journal of General Microbiology 128:1959–1969
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-129-8-2649
Loading
/content/journal/micro/10.1099/00221287-129-8-2649
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error