1887

Abstract

The effect of antimicrobial agents on the intracellular multiplication of in cultured guinea-pig peritoneal macrophages was measured. Beta-lactam antibiotics at concentrations 5 to 400 times the MIC did not inhibit the intracellular growth of the organism. Gentamicin inhibited the growth considerably but failed to eliminate the organism from the phagocytic mixture. Chloramphenicol or tetracycline at 10 μg ml (40 or 5 times the MIC respectively) did not eliminate the organism. At a higher concentration (30 μg ml), however, these drugs eliminated the bacterium from the mixture. Only erythromycin and rifampin were effective in killing the organism at very low concentration (1 μg ml). Intracellular multiplication of was observed clearly by light microscopy using Wright-Giemsa staining.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-4-901
1984-04-01
2024-05-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/4/mic-130-4-901.html?itemId=/content/journal/micro/10.1099/00221287-130-4-901&mimeType=html&fmt=ahah

References

  1. Bacheson M. A., Friedman H. M., Benson C. E. 1981; Antimicrobial susceptibility of intracellular Legionella pneumophila. Antimicrobial Agents and Chemotherapy 20:691–692
    [Google Scholar]
  2. Braude A. I. 1981; Description of antimicrobial drugs. In Medical Microbiology and Infectious Diseases pp. 210–234 Braude A. I. Edited by Philadelphia: W. B. Saunders Co;
    [Google Scholar]
  3. Edelstein P. H., Mayer R. D. 1980; Susceptibility of Legionella pneumophila to twenty antimicrobial agents. Antimicrobial Agents and Chemotherapy 18:403–408
    [Google Scholar]
  4. Feeley J. C., Gibson R. J., Gorman G. W., Langford N. C., Rasheed J. K., Mackel D. C., Bain W. B. 1979; Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. Journal of Clinical Microbiology 10:437–441
    [Google Scholar]
  5. Fraser D. W., Wachsmuth I. K., Bopp C., Feeley J. C., Tsai T. F. 1978; Antibiotic treatment of guinea pig infected with agent of Legionnaires’ disease. Lancet 1:175–177
    [Google Scholar]
  6. Fu K. P., Neu H. C. 1979; Inactivation of β-lactam antibiotics by Legionella pneumophila. Antimicrobial Agents and Chemotherapy 16:561–565
    [Google Scholar]
  7. Horwitz M. A., Silverstein S. C. 1980; Legionnaires’ disease bacterium (Legionella pneumophila) multiplies intracellularly in human monocytes. Journal of Clinical Investigation 66:441–450
    [Google Scholar]
  8. Horwitz M. A., Silverstein S. C. 1981; Interactions of the Legionnaires’ disease bacterium (Legionella pneumophila) with human phagocytes. I.L. pneumophila resists killing by polymorphonuclear leucocytes, antibody and complement. Journal of Experimental Medicine 153:386–397
    [Google Scholar]
  9. Johnson J. D., Hand W. L., Francis J. B., King-Thompson N., Corwin R. W. 1980; Antibiotic uptake by alveolar macrophages. Journal of Laboratory and Clinical Medicine 95:429–439
    [Google Scholar]
  10. Kirby B. D., Snyder K. M., Meyer R. D., Finegold S. M. 1980; Legionnaires’ disease: report of sixty-five nosocomially acquired cases and review of the literature. Medicine 59:188–205
    [Google Scholar]
  11. Kishimoto R. A., White J. D., Shirey F. G., McGann V. G., Berendt R. F., Larson E. W., Hedlund K. W. 1981; In vitro response of guinea pig peritoneal macrophages to Legionella pneumophila. Infection and Immunity 31:1209–1213
    [Google Scholar]
  12. Prokesh R. C., Hand W. L. 1982; Antibiotic entry into human polymorphonuclear leukocytes. Antimicrobial Agents and Chemotherapy 21:373–380
    [Google Scholar]
  13. Ristroph J. D., Hedlund K. W., Allen R. G. 1980; Liquid medium for growth of Legionella pneumophila. Journal of Clinical Microbiology 11:19–21
    [Google Scholar]
  14. Sabath L. D. 1980; Peak serum concentrations frequently obtained with some antibiotics. In Manual of Clinical Microbiology, 3rd edn. p. 500 Lennette E. H. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  15. Wong M. C., Ewing E. P. Jr Callaway C. S., Peacock W. L. Jr 1980; Intracellular multiplication of Legionella pneumophila in cultured human embryonic lung fibroblasts. Infection and Immunity 28:1014–1018
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-4-901
Loading
/content/journal/micro/10.1099/00221287-130-4-901
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error