1887

Abstract

Three strains of Gram-negative, obligately aerobic, moderately thermophilic, CO-utilizing bacteria have been isolated by enrichment at 45–55 °C in mineral medium under CO, H and air. The properties of the strains, designated C2, SI and FE, suggest that they should be included in a single species that we have named . The isolates can grow on CO and a limited range of organic and amino acids; neither sugars nor H/CO support growth and H is not oxidized. Strain C2 contains a soluble, inducible CO. phenazine etho-sulphate oxidoreductase, lacks hydrogenase and assimilates CO via ribulose bisphosphate carboxylase. The organism apparently contains a mechanism for assimilating phospho-glycollate.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-5-1097
1984-05-01
2024-05-17
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/5/mic-130-5-1097.html?itemId=/content/journal/micro/10.1099/00221287-130-5-1097&mimeType=html&fmt=ahah

References

  1. Anderson L., Tolbert N. E. 1966; Phosphoglycollate phosphatase. Methods in Enzymology 9:646–650
    [Google Scholar]
  2. Bamforth C. W., Quayle J. R. 1977; Hydroxy-pyruvate reductase activity in Paracoccus denitrificans. Journal of General Microbiology 101:259–267
    [Google Scholar]
  3. Buedeker R. F., Kuenen J. G., Codd G. A. 1981; Glycollate metabolism in the obligate chemolithotroph Thiobacillus neapolitanus grown in continuous culture. Journal of General Microbiology 126:337–346
    [Google Scholar]
  4. Codd G. A., Stewart W. D. P. 1973; Pathways of glycollate metabolism in the blue-green alga Anabaena cylindrica. Archiv für Mikrobiologie 94:11–28
    [Google Scholar]
  5. Colby J., Zatman L. J. 1975; Enzymological aspects of the pathways for trimethylamine oxidation and C1-assimilation in obligate and restricted facultative methylotrophs. Biochemical Journal 148:513–520
    [Google Scholar]
  6. Colby J., Dalton H., Whittenbury R. 1979; Biological and biochemical aspects of microbial growth on Ct -compounds. Annual Reviews of Microbiology 33:481–517
    [Google Scholar]
  7. Colby J., Stirling D. I., Dalton H. 1977; Soluble methane mono-oxygenase of Methylococcuscapsulatus (Bath); its ability to oxygenate alkanes, alkenes, ethers, alicyclic, aromatic and heterocyclic compounds. Biochemical Journal 165:395–402
    [Google Scholar]
  8. Collins C. H., Lyne P. M. 1970 Microbiological Methods, 3rd edn.. London:: Butterworths.;
    [Google Scholar]
  9. Conrad R., Seiler W. 1980; Role of microorganisms in the consumption and production of atmospheric carbon monoxide by soil. Applied and Environmental Microbiology 40:437–445
    [Google Scholar]
  10. Conrad R., Seiler W. 1982; Utilization of traces of carbon monoxide by aerobic oligotrophic microorganisms in oceans, lakes and soil. Archives of Microbiology 132:41–46
    [Google Scholar]
  11. Herbert D., Phipps P. J., Strange R. E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  12. Kennedy S. I. T, Fewson C. A. 1968; Enzymes of the mandelate pathway in bacterium NCIB 8250. Biochemical Journal 107:497–506
    [Google Scholar]
  13. Kim Y. M., Hegeman G. D. 1981; Purification and some properties of the carbon monoxide dehydrogenase from Pseudomonas carboxydohydrogena. Journal of Bacteriology 148:904–911
    [Google Scholar]
  14. Kim Y. M., Hegeman G. D. 1983; Oxidation of carbon monoxide by bacteria. International Review of Cytology 81:1–32
    [Google Scholar]
  15. Kornberg J. G., Morris J. G. 1965; The utilisation of glycollate by Micrococcus denitrificans: the β-hydroxyaspartate pathway. Biochemical Journal 95:577–586
    [Google Scholar]
  16. Marmur J. 1961; A procedure for the isolation of DNA from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  17. Marmur J., Doty P. 1962; Determination of the base composition of DNA from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  18. Meyer O., Schlegel H. G. 1983; Biology of aerobic carbon monoxide-oxidising bacteria. Annual Review of Microbiology 37:277–310
    [Google Scholar]
  19. Norris J. R., Swain H. 1971; Staining bacteria. Methods in Microbiology 5A:105–134
    [Google Scholar]
  20. Ostle A. G., Holt J. G. 1982; Nile blue A as a fluorescent stain for poly-β-hydroxybutyrate. Applied and Environmental Microbiology 44:238–241
    [Google Scholar]
  21. Owens J. D., Keddie R. M. 1969; The nitrogen nutrition of soil and herbage coryneform bacteria. Journal of Applied Bacteriology 32:338–347
    [Google Scholar]
  22. Postgate J. R. 1972; The acetylene reduction test for nitrogen fixation. Methods in Microbiology 6B:343–356
    [Google Scholar]
  23. Primrose S. B., Day M. 1977; Rapid concentration of bacteriophage from aquatic habitats. Journal of Applied Bacteriology 42:417–421
    [Google Scholar]
  24. Robbins R. C., Borg K. M., Robinson E. 1968; Carbon monoxide in the atmosphere. Journal of the Air Pollution Control Association 18:106–111
    [Google Scholar]
  25. Taylor S. C. 1977; Evidence for the presence of ribulose 1,5-bisphosphate carboxylase and phosphoribulokinase in Methylococcus capsulatus(Bath). FEMS Microbiology Letters 2:305–307
    [Google Scholar]
  26. Taylor S. C., Dalton H., Dow C. S. 1981; Ribulose-1,5-bisphosphate carboxylase/oxygenase and carbon assimilation in Methylococcus capsulatus(Bath). Journal of General Microbiology 122:89–94
    [Google Scholar]
  27. Vela G., Wyss O. 1964; Improved stain for visualisation of Azotobacter encystment. Journal of Bacteriology 87:476–477
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-5-1097
Loading
/content/journal/micro/10.1099/00221287-130-5-1097
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error