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Glutamine Requirement for Aerial Mycelium Growth in Neurospora crassa
Abstract
Five amino acids are accumulated during vegetative growth of Neurospora crassa, particularly during the prestationary growth phase. Alanine, glutamine, glutamate, arginine and ornithine comprised over 80% of the total amino acid pool in the mycelium. Amino acid pools of different amino acid auxotrophs were followed during the partial transformation of a mycelial mat into an aerial mycelium. The mycelial mat under starvation and in direct contact with air rapidly formed aerial mycelium, which produced thereafter a burst of conidia. During this process, glutamine and alanine in the mycelial mat were consumed more rapidly than other amino acids; in the growing aerial mycelium, glutamate and glutamine were particularly accumulated. Of the amino acids that were initially accumulated in the mycelial mat, only a high glutamine pool was required for aerial mycelium growth induced by starvation. This requirement for glutamine could not be satisfied by a mixture of the amino compounds that are synthesized via glutamine amidotransferase reactions. It is proposed that glutamine serves as a nitrogen carrier from the mycelial mat to the growing aerial mycelium.
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© Society for General Microbiology 1984