1887

Abstract

Bacteriophages have been induced from strains in the serocomplex by exposure of cultures to UV light or treatment with mitomycin C. One-sixth of the strains examined, representing all but one of the 31 authenticated serotypes, were found to possess phages lytic for a indicator strain. Four single-plaque isolated phages, TM4, TM9, TM10 and TM20, were purified and shown to have a similar morphology on electron micrographs. They had an isometric head of diameter 50–58 nm and a flexible non-contractile tail about 170 nm in length with a terminal bulb. All had an identical buoyant density in CsCl of 1·521 g cm and extreme sensitivity to chloroform. The induced phages differed in host range and possessed the unique ability to lyse other members of the serocomplex. Interest in these phages centres on a possible role in mediating genetic interrelationships between members of the serocomplex.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-130-8-2059
1984-08-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/130/8/mic-130-8-2059.html?itemId=/content/journal/micro/10.1099/00221287-130-8-2059&mimeType=html&fmt=ahah

References

  1. Baess I. 1970; Report on a pseudolysogenic mycobacterium and a review of the literature concerning pseudolysogeny. Acta pathologica et microbiologica scandimvica B79:428–434
    [Google Scholar]
  2. Barrow W. W., Ullom B. P., Brennan P. J. 1980; Peptidoglycolipid nature of the superficial cell wall sheath of smooth-colony-forming mycobacteria. Journal of Bacteriology 144:814–822
    [Google Scholar]
  3. Bradley D. E., Rutherford E. L. 1975; Basic characterization of a lipid containing bacteriophage specific for plasmids of the P, N, and W compatabi- lity groups. Canadian Journal of Microbiology 21:152–163
    [Google Scholar]
  4. Brennan P. J., Goren M. B. 1979; Structural studies on the type-specific antigens and lipids of the Mycobacterium avium/Mycobacterium intracellularej Mycobacterium scrofulaceum serocomplex. Journal of Biological Chemistry 254:4205–4211
    [Google Scholar]
  5. Brennan P. J., Mayer H., Aspinall G. O., Namshin J. E. 1981; Structures of the glycopeptido- lipid antigens from serovars in the Mycobacterium avium/ Mycobacterium intracellularej Mycobacterium scrofulaceum serocomplex. European Journal of Biochemistry 115:7–15
    [Google Scholar]
  6. Brennan P. J., Heifets M. J., Ullom B. P. 1982; Thin layer chromatography of lipid antigens as means of identifying nontuberculous mycobacteria. Journal of Clinical Microbiology 15:447–455
    [Google Scholar]
  7. Brunner R., Vinograd J. 1965; The evalution of standard sedimentation coefficients of sodium RNA and sodium DNA from sedimentation velocity data in concentrated NaCl and CsCl solutions. Biochimica et biophysica acta 108:18–29
    [Google Scholar]
  8. Buraczewska M., Manowska W., Rdultowska H. 1971; Induction of phage from lysogenic mycobacteria by means of ultraviolet irradiation. American Review of Respiratory Diseases 104:760–762
    [Google Scholar]
  9. Buraczewska M., Kwiatkowski B., Manowska W., Rdultowska H. 1972; Ultrastructure of some mycobacteriophages. American Review of Respiratory Diseases 105:22–29
    [Google Scholar]
  10. Cohn M. L., Waggoner R. F., Mcclatchy J. K. 1968; The 7H11 medium for the cultivation of mycobacteria. American Review of Respiratory Diseases 98:1064–1067
    [Google Scholar]
  11. Crawford J. T., Fitzhugh J. K. 1981; Phage typing of the Mycobacterium avium/intracellulare-scrofulaceum complex. American Review of Respiratory Diseases 124:559–562
    [Google Scholar]
  12. Draper P. 1974; The mycoside capsule of Mycobacterium avium 357. Journal of General Microbiology 83:431–433
    [Google Scholar]
  13. Draper P. 1982; The physiology of mycobacteria. In The Biology of the Mycobacteria I pp. 9–52 Ratledge C., Stanford J. Edited by London & New York: Academic Press;
    [Google Scholar]
  14. Draper P., Rees R. J. W. 1973; The nature of the electron-transparent zone that surrounds Mycobacterium lepraemurium inside host cells. Journal of General Microbiology 77:79–87
    [Google Scholar]
  15. Furuchi A., Tokunaga T. 1972; Nature of the receptor substance of Mycobacterium smegmatis for D4 bacteriophage adsorption. Journal of Bacteriology 14:404–409
    [Google Scholar]
  16. Goren M. B., Mcclatchy J. K., Martens B., Brokl O. 1972; Mycosides C: behaviour as receptor site substance for mycobacteriophage D4. Journal of Virology 9:999–1003
    [Google Scholar]
  17. Grange J. M. 1982; The genetics of mycobacteria and mycobacteriophages. In The Biology of the Mycobacteria I pp. 309–351 Ratledge C., Stanford J. Edited by London & New York: Academic Press;
    [Google Scholar]
  18. Holmes D., Wojtkiewicz P., Barridge B. D. 1981; Properties of the virulent form of a mitomycin C- or temperature-induced thermophilic bacteriophage. Journal of General Virology 52:141–144
    [Google Scholar]
  19. Imaeda T., Rieber M. 1968; Mitomycin C-induced phage-like particles in a mutant of Mycobacterium tuberculosis BCG. Journal of Bacteriology 96:557–559
    [Google Scholar]
  20. Jones W. D., Greenberg J. 1978; Modification of methods used in bacteriophage typing of Mycobacterium tuberculosis isolates. Journal of Clinical Microbiology 7:467–469
    [Google Scholar]
  21. Rado T. A., Bates J. H. 1980; Mycobacteriophage structure and function: a review. Advances in Tuberculosis Research 20:64–91
    [Google Scholar]
  22. Russell R. L., Jann G. J., Froman S. 1960; Lysogeny in the mycobacteria. I. Establishment of lysogeny. American Review of Respiratory Diseases 82:384–393
    [Google Scholar]
  23. Tageldin M. H., Elhassan A. M., Mustafa I. E. 1981; Spontaneous release of mycophages from lysogenic bovine strains. Tubercule 62:263–269
    [Google Scholar]
  24. Takeya K., Tokiwa H. 1972; Mycobacteriocin classification of rapidly growing mycobacteria. International Journal of Systematic Bacteriology 22:178–180
    [Google Scholar]
  25. Takeya K., Yoshimura T., Yamamura K., Toda T. 1959; Studies on the biologic properties of mycobacteriophage. American Review of Respiratory Diseases 80:543–553
    [Google Scholar]
  26. Tsang A. Y., Drupa I., Goldberg M., Mcclatchy J. K., Brennan P. J. 1983; Use of serology and thin-layer chromatography for the assembly of an authenticated collection of serovars within the Mycobacterium avium-Mycobacterium intraceilulare-Mycobacterium scrofulaceum complex. International Journal of Systematic Bacteriology 33:285–292
    [Google Scholar]
  27. Wolinsky E. 1979; Nontuberculous mycobacteria and associated diseases. American Review of Respiratory Diseases 119:107–159
    [Google Scholar]
  28. Wolinsky E., Schaefer W. B. 1973; Proposed numbering scheme for mycobacterial serotypes by agglutination. International Journal of Systematic Bacteriology 23:182–183
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-130-8-2059
Loading
/content/journal/micro/10.1099/00221287-130-8-2059
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error