1887

Abstract

Summary: A novel method for the isolation of mutants partially defective in haem synthesis is described. Mutant clones were identified by their fluorescence under UV light due to the accumulation of porphyrins in cells, and by their ability to grow on nonfermentable carbon sources due to their preserved haemoprotein synthesis. Thirteen such mutants were obtained by this procedure. The defects in haem synthesis and accumulation of porphyrins in all the mutants were confirmed by spectrophotometric analysis. Complementation tests with biochemically defined, haem-less strains showed that in seven mutants uroporphyrinogen decarboxylase was affected and that in three mutants the defect concerned ferrochelatase. The defects in the remaining three mutants were not defined.

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1985-11-01
2024-04-27
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References

  1. Bassel J., Hambright P., Mortimer R., Bearden J. A. 1975; Mutant of the yeast Saccharomycopsis lipolytica that accumulates and excretes protoporphyrin IX. Journal of Bacteriology 123:118–122
    [Google Scholar]
  2. BilińSKI T., Litwińska J., Sledziewski A., Rytka J. 1980; Genetic analysis of pleiotropic mutants affected in the response to glucose repressiosion and anoxia. Acta microbiologica polonica 29:199–212
    [Google Scholar]
  3. Biliński T., Litwińska J., Łukaszkiewicz J., Rytka J., Simon M., Labbe-Bois R. 1981; Characterization of two mutant strains of Saccharomyces cerevisiae deficient in coproporphyrinogen III oxidase activity. Journal of General Microbiology 122:79–87
    [Google Scholar]
  4. Camadro J.-M., Labbe P. 1982; Kinetic studies of ferrochelatase in yeast. Zinc or iron as competing substrates. Biochimica et biophysica acta 707:280–288
    [Google Scholar]
  5. Camadro J.-M., Urban-Grimal D., Labbe P. P. 1982; A new assay for protoporphyrinogen oxidase - evidence for a total deficiency in that activity in a heme-less mutant of Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 106:724–730
    [Google Scholar]
  6. Chaix P., Labbe P. 1965; A propos de l’interprétation du spectra d’absorption de cellules de levures récoltées à la fin ou après la phase exponentielle de leur croissance anaérobie. In Mecacanismes de Régulation des Activités Cellulair chez les Microorganismes Colloque International, Marseille, 1963), pp. 481–490 Paris: Centre National de la Recherche Scientifique;
    [Google Scholar]
  7. Doss M. O. 1982; Hepatic porphyrias: pathobio-chemical, diagnostic and therapeutic implications. In Progress in Liver Diseases vol VII pp 573–597 Edited by Popper H., Schaffner F. Grune … Stratton: New York;
    [Google Scholar]
  8. Elder G. H. 1978; Porphyria caused by hexachloro-benzene and other polyhalogenated aromatic hydrocarbons. In Heme and Hemoprote pp 157–200 Edited by De Matteis F., Aldrige W. N. Berlin, Heidelberg … New York: Springer Verlag;
    [Google Scholar]
  9. Falk J. E. 1964 Porphyrines and Metalloporphyri BBA Library, vol 2 Amsterdam, London & New York: Elsevier;
    [Google Scholar]
  10. Fink G. R. 1970; The biochemical genetics of yeast. Methods in Enzymology 17A:59–78
    [Google Scholar]
  11. Gollub E. G., Liu K., Dayan J., Adlersberg M., Sprinson D. B. 1977; Yeast mutants deficient in heme biosynthesis and a heme mutant additionally blocked in cyclization of 2,3-oxidosqualene. Journal of Biological Chemistry 252:2846–2854
    [Google Scholar]
  12. Labbe-Bois R., Labbe P. 1978; Protohaem synthesis in Saccharomyces cerevisiae. In Biochemistry and Genetics of Yeasts pp 97–117 Edited by Bacilla M., Horecker B. L., Stoppani A. O. M. New York & London: Academic Press;
    [Google Scholar]
  13. Labbe-Bois R., Rytka J., Litwińska J., Biliński T. 1977; Analysis of heme biosynthesis in catalase and cytochrome deficient mutants. Molecular and General Genetics 156:177–183
    [Google Scholar]
  14. Labbe-Bois R., Urban-Grimal D., Volland C., Camadro J., Dehoux P. 1983; About the regulation of protoheme synthesis in the yeast Saccharomyces cerevisiae. In Mitochondria 2983 pp 523–534 Edited by Schweyen R. J., Wolf K., Kaudewitz F. Kaudewitz. Berlin & New York: Walter de Gruyter;
    [Google Scholar]
  15. Mijake S., Iwamoto Y., Nago M., Sugimura T., Ohsumi M. 1972; Phenotype of a temperature sensitive respiration-deficient (cyt mutant of yeast. Journal of Bacteriology 109:409–415
    [Google Scholar]
  16. Mortimer R.K., Hawthorne D.C. 1969; Yeast genetics. In The Yeasts vol I pp 385–460 Edited by Rose A. H., Harrison J. S. London & New York: Academic Press;
    [Google Scholar]
  17. Pachecka J., Litwińska J., Biliński T. 1974; Hemoprotein formation in yeast. I. Isolation of catalase- and cytochrome-deficient mutants. Molecular and General Genetics 134:299–305
    [Google Scholar]
  18. Pretlow T. P., Sherman F. 1967; Porphyrins and zinc-porphyrins in normal and mutant strains of yeast. BiocBiochimica et biophysica acta 148:629–644
    [Google Scholar]
  19. Rytka J., Sledziewski A., Łukaszkiewicz J., Biliński T. 1978; Haemoprotein formation in yeast. III. The role of carbon catabolite repression in the regulation of catalase A and T formation. Molecular and General Genetics 160:51–57
    [Google Scholar]
  20. Rytka J., Biliński T., Labbe-Bois R. 1984; Modified uroporphyrinogen decarboxylase activity in a yeast mutant which mimics porphyria cutanea tarda. Biochemical Journal 218:405–413
    [Google Scholar]
  21. Sassa S., Kappas A. 1981; Genetic, metabolic and biochemical aspects of the porphyrias. Advances in Human Genetics 11:121–231
    [Google Scholar]
  22. Taketani S., Tokunaga R. 1981; Rat liver ferrochelatase. Purification, properties and stimulation by fatty acids. Journal of Biological Chemistry 256:12748–12753
    [Google Scholar]
  23. Taketani S., Tokunaga R. 1984; Non-enzymatic heme formation in the presence of fatty acids and thiol reductants. Biochimica et biophysica acta 798:226–230
    [Google Scholar]
  24. Urban-grimal D., Labbe-Bois R. 1981; Genetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis. Molecular and General Genetics 183:85–92
    [Google Scholar]
  25. Woods R. A., Sanders H. K., Briquet M., Foury F., Drysdale B. E., Matoon J. R. 1975; Regulation of mitochondrial biogenesis: enzymatic changes in cytochrome-deficient yeast mutants requiring δ-aminolevulinic acid. Journal of Biological Chemistry 250:9090–9098
    [Google Scholar]
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