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Abstract
Summary: The accumulation of amino acids and the excretion of ammonium have been studied in various Neurospora crassa ammonium assimilation mutants, together with the labelling of glutamine in the presence of [U-14C]sucrose when N. crassa grows on glutamine as the nitrogen source. Ammonium coming from glutamine degradation by the ω-amidase pathway is assimilated by NADP-dependent glutamate dehydrogenase and glutamine synthetase. The operation of these enzymes results in a cycling of glutamine that seems to be essential for cell growth. In addition, glutamine is also converted to glutamate by glutamate synthase.
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© Society for General Microbiology 1985