1887

Abstract

Summary: An alkalophilic sp., no. 1139, was isolated from soil and this bacterium produced a carboxymethyl cellulase (CMCase). The production of enzyme was strongly inhibited by the addition of glucose. The CMCase was purified on a DEAE-Toyopearl 650 ion-exchange column followed by Toyopearl HW-55F gel filtration and passage through a DEAE-Toyopearl 650 ion-exchange column. The purified enzyme gave a single band of protein on PAGE. The enzyme hydrolysed carboxymethylcellulose with an optimum at pH 9.0 and a of 0.48 mg ml; no activity was observed at pH 6.0. The enzyme had a molecular weight (SDS-PAGE) of 92000 and an isoelectric point of 3.1. The maximum degree of hydrolysis of carboxymethylcellulose was about 30% and -glucosidase activity was also observed.

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/content/journal/micro/10.1099/00221287-131-12-3339
1985-12-01
2024-04-28
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References

  1. Bencze W. L., Schmid K. 1957; Determination of tyrosine and tryptophan in proteins.. Analytical Chemistry 29:1193–1196
    [Google Scholar]
  2. Berg B., Hofsten B. V., Pettersson B. 1972; Growth and cellulose formation by Cellovibrio fulvus . Journal of Applied Bacteriology 35:201–214
    [Google Scholar]
  3. Buchanan R. H., Gibbons N. E. (editors) 1974 Bergey's Manual of Determinative Bacteriology 8th edn, pp 529–550 Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Eriksson K. E., Pettersson B. 1975; Extracellular enzymes utilized by the fungus Sporotrichum pulverulentum for the breakdown of cellulase. I. Preparation, purification and physicochemical characterization of five endo-l,4-β-glucanases.. European Journal of Biochemistry 51:193–206
    [Google Scholar]
  5. Gordon R. E., Hyde J. L. 1982; The Bacillus firmus–Bacillus lentus complex and pH 7.0 variants of some alkalophilic strains.. Journal of General Microbiology 128:1109–1116
    [Google Scholar]
  6. Gordon R. E., Haynes W. C., Pang C. H. N. 1973 The Genus Bacillus. Agriculture Handbook no. 427 Washington, DC: United States Department of Agriculture;
    [Google Scholar]
  7. Han Y. W., Srinvasan V. R. 1968; Isolation and characterization of a cellulose utilizing bacterium.. Applied Microbiology 16:1140–1145
    [Google Scholar]
  8. Horikoshi K., Akiba T. 1982 Alkalophilic Microorganisms, a New Microbial World New York: Springer-Verlag;
    [Google Scholar]
  9. Horikoshi K., Nakao M., Kurono Y., Sashihara N. 1984; Cellulase of an alkalophilic Bacillus strain isolated from soil.. Canadian Journal of Microbiology 30:774–779
    [Google Scholar]
  10. Kubo K., Nishizawa K. 1983; Purification and properties of two endo-type cellulases from Irpex lacteus (Polyporus tulipiferae).. Journal of Fermentation Technology 61:383–389
    [Google Scholar]
  11. Labudova I., Farkas V. 1983; Multiple enzyme forms in the cellulase system of Trichoderma reesei during its growth on cellulose.. Biochimica et biophysica acta 744:135–140
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4.. Nature, London 227:680–685
    [Google Scholar]
  13. Nakamura K., Kitamura K. 1982; Isolation and identification of crystalline cellulose hydrolyzing bacterium and its enzymatic properties.. Journal of Fermentation Technology 60:343–348
    [Google Scholar]
  14. Okada G., Nishizawa K. 1975; Enzymatic studies on a cellulase system of Trichoderma viride. III. Transglycosylation properties of two cellulase components of random type.. Journal of Biochemistry 78:297–306
    [Google Scholar]
  15. Sasaki T., Tanaka T., Kainuma K. 1979; A simple method for preparation of cellooligosaccharides.. Nippon shokuhin Kogyo Gakkaishi 26:538–541
    [Google Scholar]
  16. Sashihara N., Kudo T., Horikoshi K. 1984; Molecular cloning and expression of cellulase genes of alkalophilic Bacillus sp. strain N-4 in Escherichia coli . Journal of Bacteriology 158:503–506
    [Google Scholar]
  17. Sen S., Abraham T. K., Chacrabarty P. 1982; Characteristics of the celluloses produced by Myceliophthora thermophila D-14.. Canadian Journal of Microbiology 28:271–277
    [Google Scholar]
  18. Thomas K., Ng & Zeikus J. G. 1981; Purification and characterization of an endoglucanase (1,4-β-d-glucan glucanohydrolase) from Clostridium thermocellum . Biochemical Journal 199:341–350
    [Google Scholar]
  19. Yamane K., Yoshikawa T., Suzuki H., Nishizawa K. 1970; Purification and properties of extracellular and cell-bound cellulase components of Pseudomonas fluorescens var. cellulosa . Journal of Biochemistry 67:19–32
    [Google Scholar]
  20. Yoshioka H., Anraku S., Hayashida S. 1982; Production and purification of a novel type of CMCase from Humicola grisea var. thermoidea YH-78.. Agricultural and Biological Chemistry 46:75–82
    [Google Scholar]
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