1887

Abstract

undergoes yeast to mycelial conversion under both and conditions but the relative pathogenicity of the two forms of growth is still unknown. By adapting a recently developed Cr radiolabel release assay, we have quantified the killing ability of different murine effector cell populations for the hyphal form of . Up to 50% of specific Cr release from the mycelial form could be detected after incubation for only 1 h, with no requirement for opsonization, provided that appropriate effector : target cell ratios were used. The specific Cr release correlated well with viability, as assessed by dye exclusion tests, and with pathogenicity potential in cyclophosphamide-immunodepressed mice. Comparison of the activity of different murine effectors against yeast and hyphal forms showed that hyphal forms were killed by murine effectors to a similar, if not greater, extent than yeast forms. In particular, thioglycollate-induced murine polymorphonuclear neutrophils were able to kill hyphal cells extracellularly and without an opsonic requirement.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-131-3-505
1985-03-01
2024-04-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/131/3/mic-131-3-505.html?itemId=/content/journal/micro/10.1099/00221287-131-3-505&mimeType=html&fmt=ahah

References

  1. Baccarini M., Bistoni F., Puccetti P., Garaci E. 1983a; Natural cell-mediated cytotoxicity against Candida albicans induced by cyclophosphamide. Nature of the in vitro cytotoxic effector. Injection and Immunity 42:1–9
    [Google Scholar]
  2. Baccarini M., Blasi E., Puccetti P., Bistoni F. 1983b; Phagocytic killing of Candida albicans by different murine effector cells. Sabouraudia 21:271–286
    [Google Scholar]
  3. Bistoni F., Baccarini M., Blasi E., Puccetti P., Marconi P. 1982; A radiolabel release microassay for phagocytic killing of Candida albicans . Journal of Immunological Methods 52:369–377
    [Google Scholar]
  4. Bistoni F., Baccarini M., Blasi E., Puccetti P., Marconi P., Garaci E. 1983; Correlation between ‘in vivo’ and ‘in vitro’ studies of modulation of resistance to experimental Candida albicans infection by cyclophosphamide in mice. Infection and Immunity 40:46–55
    [Google Scholar]
  5. Braun P. C., Calderone R. A. 1978; Chitin synthesis in Candida albicans: comparison of yeast and hyphal forms. Journal of Bacteriology 133:1472–1477
    [Google Scholar]
  6. Cassone A., Simonetti N., Strippoli V. 1973; Ultrastructural changes in the wall during germ-tube formation from blastospores of Candida albicans . Journal of General Microbiology 77:417–426
    [Google Scholar]
  7. Chattaway F. W., Holmes M. R., Barlow A. J. E. 1968; Cell wall composition of the mycelial and blastospore forms of Candida albicans . Journal of General Microbiology 51:367–376
    [Google Scholar]
  8. Cockayne A., Odds F. C. 1984; Interaction of Candida albicans yeast cells, germ tubes and hyphae with human polymorphonuclear leukocytes in vitro. Journal of General Microbiology 130:465–471
    [Google Scholar]
  9. Dabrowa N., Taxer S. S., Howard D. 1976; Germination of Candida albicans induced by proline. Infection and Immunity 13:830–835
    [Google Scholar]
  10. Davies R. R., Denning T. J. V. 1972; Candida albicans and the fungicidal activity of the blood. Sabouraudia 10:301–312
    [Google Scholar]
  11. Diamond R. D., Wrzesicki R., Wellington J. 1978; Damage to pseudohyphal forms of Candida albicans by neutrophils in the absence of serum ‘in vitro’. Journal of Clinical Investigations 61:349–359
    [Google Scholar]
  12. Gould T. J. L., Schotte C., van Fourth R. 1975; Identification and characterization of the monoblast in mononuclear phagocyte colonies grown ‘in vitro’. Journal of Experimental Medicine 142:1180–1199
    [Google Scholar]
  13. Kagaya K., Fukazawa Y. 1981; Murine defense mechanisms against Candida albicans infection. II. Opsonization, phagocytosis and intracellular killing of C. albicans. Microbiology and Immunology 25:807–818
    [Google Scholar]
  14. Lehrer R. I., Cline M. J. 1969; Interaction of Candida albicans with human leukocytes and serum. Journal of Bacteriology 98:998–1004
    [Google Scholar]
  15. Lodder J. 1970 The Yeasts: A Taxonomic Study914 Amsterdam, London: North Holland Publishing Company;
    [Google Scholar]
  16. Marconi P., Bistoni F., Boncio L., Bersiani A., Bravi P., Pitzurra M. 1976; Utilizzazione di una soluzione salino ipertonica di cloruro di potassio (3M KCL) per l’estrazione di antigeni solubili da Candida albicans. Annali sclavo 18:61–66
    [Google Scholar]
  17. Mattia E., Cassone A. 1979; Inducibility of germ-tube formation in Candida albicans at different phases of yeast growth. Journal of General Microbiology 113:439–442
    [Google Scholar]
  18. Mattia E., Carruba C., Angiolella L., Cassone A. 1982; Induction of germ-tube formation by N-acetyl-d-glucosamine in Candida albicans: uptake of inducer and germinative response. Journal of Bacteriology 152:555–562
    [Google Scholar]
  19. Odds F. C. 1979 Candida and Candidosis Leicester: Leicester University Press;
    [Google Scholar]
  20. Parker J. C., McCloskey J. J., Knauer K. A. 1976; Pathobiologic features of human candidiasis. A common deep mycosis of the brain, heart and kidney in the altered host. American Journal of Clinical Pathology 65:991–999
    [Google Scholar]
  21. Rogers T. S., Balish E. 1980; Immunity to Candida albicans . Microbiology Reviews 44:660–682
    [Google Scholar]
  22. Saltarelli C. G., Gentile K. A., Mancuso S. C. 1975; Lethality of Candida strains as influenced by the host. Canadian Journal of Microbiology 21:648–653
    [Google Scholar]
  23. Shepherd M. G., Chiew Y. Y., Ram S. P., Sullivan P. A. 1980; Germ-tube induction in Candida albicans. Canadian Journal of Microbiology 26:21–26
    [Google Scholar]
  24. Simonetti N., Strippoli V., Cassone A. 1974; Yeast-mycelial conversion induced by N-acetyl-d-glucosamine in Candida albicans . Nature London: 250:344–346
    [Google Scholar]
  25. White L. O., Gibb E., Newham H. C., Richardson M. D., Warren R. C. 1979; Comparison of the growth of virulent and attenuated strains of Candida albicans in the kidneys of normal and cortisone-treated mice by chitin assay. Mycopathologia 67:173–176
    [Google Scholar]
  26. Winblad B. 1975; Experimental renal candidiasis in mice and guinea pigs. Acta pathologica et microbiologica scandinavica 83:400–407
    [Google Scholar]
  27. Yamamura M., Boler J., Valdimarsson H. 1977; Phagocytosis measured as inhibition of uridine uptake by Candida albicans . Journal of Immunological Methods 14:19–24
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-131-3-505
Loading
/content/journal/micro/10.1099/00221287-131-3-505
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error