f Three Restriction Endonucleases from Anabaena flos-aquae
- Authors: PHILIP R. WHITEHEAD†, NIGEL L. BROWN
- Microbiology, April 1985 131: 951-958, doi: 10.1099/00221287-131-4-951
- Subject: Genetics And Molecular Biology
- Published Online:
SUMMARY: Three site-specific endonucleases, AflI, AflII and AflIII, have been partially purified from the cyanobacterium Anabaena flos-aquae CCAP 1403/13f. Their recognition and cleavage specificities have been determined to be:
AflII and AflIII are new specificities and may be useful in molecular cloning, as well as in the analysis of DNA. The distribution of type II restriction endonucleases in the cyanobacteria is briefly discussed.
Present address: Biogen SA, Route de Troinex 3, 1227 Carouge, Geneva, Switzerland.
© Society for General Microbiology 1985 | Published by the Microbiology Society
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