1887

Abstract

Summary: An intracellular pectinolytic enzyme was isolated from a cell extract of and purified. The optimum pH for enzyme activity was 5·6. The enzyme preferentially degraded de-esterified substrates by hydrolysis of monosaccharide units from the non-reducing end; the only product of degradation was D-galacturonic acid. Values of and for oligo- and polygalacturonates indicated that the best substrate was digalacturonic acid; oligogalact-uronates containing either a saturated or a Δ4,5-unsaturated non-reducing end were both degraded. The enzyme was classified as an exo-D-galacturonanase [poly(1,4-α-D-galacturonide) galacturonohydrolase (EC 3.2.1.67)].

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/content/journal/micro/10.1099/00221287-131-8-2053
1985-08-01
2024-04-30
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References

  1. Barash I., Angel E. 1970; Isolation and properties of an exopolygalacturonase produced by Penicillium digitatum during infection of lemon fruits. Israel Journal of Botany 19:599–608
    [Google Scholar]
  2. Grossmann E. 1968; Zur Bildung pektischer und zellulolytischer Enzyme durch Phytophthora infestans de By. Phytopathologische Zeitschrift 62:372–382
    [Google Scholar]
  3. Hatanaka C., Ozawa J. 1969; Enzymic degradation of pectic acid. XII. Structure of the deoxysugar produced from unsaturated oligo- and polygalacturonic acids by an exopolygalacturonase of Aspergillus niger . Journal of the Agricultural Chemical Society of Japan 43:139–144
    [Google Scholar]
  4. Heinrichová K. 1981; Purification and partial characterization of another exo-d-galacturonanase of Aspergillus niger . Biologia (Bratislava) 36:197–202
    [Google Scholar]
  5. Heinrichová K. 1983; Preparation of oligogalacturonic acids by enzymatic hydrolysis. Biologia (Bratislava) 38:335–343
    [Google Scholar]
  6. Heinrichová K., Rexová-Benková L. 1976; Purification and characterization of an extracellular exo-d-galacturonanase of Aspergillus niger . Biochimica et biophysica acta 422:349–356
    [Google Scholar]
  7. Kimura H., Mizushima S. 1973; Exopolygalacturonase from a strain of Acrocylindrium . Agricultural and Biological Chemistry 37:2589–2593
    [Google Scholar]
  8. Koller A., Neukom H. 1964; Detection of oligogalacturonic acids by thin-layer chromatography. Biochimica et biophysica acta 83:366–367
    [Google Scholar]
  9. McCarthy R. E., Kotarski S. F., Salyers A. A. 1985; Location and characteristics of enzymes involved in the breakdown of polygalacturonic acid by Bacteroides thetaiotaomicron . Journal of Bacteriology 161:493–499
    [Google Scholar]
  10. Mill P. J. 1966a; The pectic enzymes of Aspergillus niger. A mercury-activated exopolygalacturonase. Biochemical Journal 99:557–561
    [Google Scholar]
  11. Mill P. J. 1966b; The pectic enzymes of Aspergillus niger. A second exopolygalacturonase. Biochemical Journal 99:562–565
    [Google Scholar]
  12. Rhodes C., Germershausen J., Suskind S. R. 1971; Neurospora crassa: preparative scale for biochemical studies. Methods in Enzymology 22:80–86
    [Google Scholar]
  13. Somogyi M. 1952; Notes on sugar determination. Journal of Biological Chemistry 195:19–23
    [Google Scholar]
  14. Wojciechowicz M. 1971; Partial characterization of pectinolytic enzymes of Bacteroides ruminicola isolated from the rumen of a sheep. Acta microbiologica polonica, Ser. A 3:2045–56
    [Google Scholar]
  15. Wojciechowicz M. 1972; Comparison of the action of Bacteroides ruminicola polygalacturonic acid lyase and pectinase on lower oligogalacturonides. Acta microbiologica polonica, Ser. A 4:21189–195
    [Google Scholar]
  16. Wojciechowicz M., Ziolecki A. 1979; Pectinolytic enzymes of large rumen treponemes. Applied and Environmental Microbiology 37:136–142
    [Google Scholar]
  17. Wojciechowicz M., Ziolecki A. 1984; A note on the pectinolytic enzyme of Streptococcus bovis . Journal of Applied Bacteriology 56:515–518
    [Google Scholar]
  18. Wojciechowicz M., Heinrichová K., Ziolecki A. 1980; A polygalacturonate lyase produced by Lachnospira multiparus, isolated from the bovine rumen. Journal of General Microbiology 117:193–199
    [Google Scholar]
  19. Wojciechowicz M., Heinrichová K., Ziolecki A. 1982; An exopectate lyase of Butyrivibrio fibrisolvens from the bovine rumen. Journal of General Microbiology 128:2661–2665
    [Google Scholar]
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