1887

Abstract

The genes encoding both subunits of the succinyl-CoA synthetase of have been identified as distal genes of the operon, which also encodes the dehydrogenase (Elo; ) and succinyltransferase (E20; ) components of the 2-oxoglutarate dehydrogenase complex. The newly defined genes express polypeptides of 41 kDa () and 31 kDa (), corresponding to the and subunits of succinyl-CoA synthetase, respectively. The genes are thus located at 16·8 min in the linkage map, together with the citrate synthase () and succinate dehydrogenase () genes, in a cluster of nine citric acid cycle genes: . Four deletion strains lacking all of these citric acid cycle enzymes were characterized. The succinyl-CoA synthetase activities of strains harbouring plasmids containing the and genes were amplified some fourfold. Further emzymological studies indicated that expression of succinyl-CoA synthetase is coordinately regulated with 2-oxoglutarate dehydrogenase.

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1986-06-01
2024-05-03
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References

  1. Amarasingham C. R., Davis B. D. 1965; Regulation of a-ketoglutarate dehydrogenase formation in Escherichi coli.. Journal of Biological Chemistry 240:3664–3668
    [Google Scholar]
  2. Bachmann B. J. 1983; Linkage map of Escherichia coli K. Microbiological Reviews Editor 12: 47:180230
    [Google Scholar]
  3. Bridger W. A. 1974; Succinyl-CoA synthetase.. In The Enzymes 3rd edn, vol.10: pp. 581–606 Editor Boyer P. D. New York: Academic Press.;
    [Google Scholar]
  4. Bridger W. A., Ramaley R. F., Boyer P. D. 1969; Succinylcoenzyme A synthetase from Escherichia coli.. Methods in Enzymology 13:70–75
    [Google Scholar]
  5. Buck D., Spencer M. E., Guest J. R. 1985; Primary structure of the succinyl-CoA synthetase of Escherichia coli.. Biochemistry 24:6245–6252
    [Google Scholar]
  6. Creaghan I. T., Guest J. R. 1972; Amber mutants of the a-ketoglutarate dehydrogenase gene of Escherichia coli K12. Journal of General Microbiology 71:207–220
    [Google Scholar]
  7. Creaghan I. T., Guest J. R. 1978; Succinate dehydrogenase-dependent nutritional requirement of the succinate dehydrogenase of Escherichia coli.. Journal of General Microbiology 107:1–13
    [Google Scholar]
  8. Darlison M. G., Guest J. R. 1984; Nucleotide sequence encoding the iron-sulphur protein subunit of the succinate dehydrogenase of Escherichia coli.. Biochemical Journal 223:507–517
    [Google Scholar]
  9. Darlison M. G., Spencer M. E., Guest J. R. 1984; Nucleotide sequence of the sue A gene encoding the 2-oxoglutarate dehydrogenase of Escherichia coli K12European. Journal of Biochemistry 141:351–359
    [Google Scholar]
  10. DRETZEN G., BELLARD M., SASSONE-CORSI P., Chambon P. 1981; A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.. Analytical Biochemistry 112:295–298
    [Google Scholar]
  11. Froehlich B., Epstein W. 1981; Escherichia coli mutants in which transcription is dependent on recA function.. Journal of Bacteriology 147:1117–1120
    [Google Scholar]
  12. Gibson J., Upper C. D., Gunsalus I. C. 1967; Succiriyl Coenzyme A synthetase from Escherichia coli.. Journal of Biological Chemistry 242:2474–2477
    [Google Scholar]
  13. Gray C.T., Wimpenny J. W. T., Mossman M. R. 1966; Effects of aerobiosis, anaerobiosis and nutrition on the formation of Krebs cycle enzymes in Escherichia coli.. Biochimica et biophysica acta 117:33–41
    [Google Scholar]
  14. Guest I. R. 1981; Hybrid plasmids containing the citrate synthetase gene (gitA) of Escherichia coli K12. Journal of General Microbiology 124:17–23
    [Google Scholar]
  15. Guest J. R., Cole S. T., Jeyaseelan K. 1981; Organization and expression of the pyruvate dehydrogenase complex genes of Escherichia coli K12. Journal of General Microbiology 127:65–79
    [Google Scholar]
  16. Higgins C. J., Ames G. F., -L., Barnes W., M-Clement J. N., Hofnung M. 1982; A novel intercistronic regulatory element of prokaryotic operons.. Nature, London 298:760–762
    [Google Scholar]
  17. Knight E., Gunsalus I. C. 1962; Formation and breakdown of acyl lipoic acids.. Methods in Enzymology S651–656
    [Google Scholar]
  18. Langley D., Guest J. R. 1977; Biochemical genetics of the a-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and biochemical properties of deletion mutants.. Journal of General Microbiology 99:263–276
    [Google Scholar]
  19. Langley D., Guest J. R. 1978; Biochemical genetics of the a-keto acid dehydrogenase complexes of Escherichia coli K12: genetic characterization and regulatory properties of deletion mutants.. Journal of General Microbiology 106:103–117
    [Google Scholar]
  20. Ner S. S., Bhayana V., Bell A. W., Giles I. G., Duckworth H. W., Bloxham D. P. 1983; Complete sequence of the git A gene encoding citrate synthase in Escherichia coli. .Biochemistry 22:5243–5249
    [Google Scholar]
  21. QUEEN C., ROSENBERG M. 1981; A promoter of pBR322 activated by cAMP receptor protein.. Nucleic Acid Research 9:3365–3377
    [Google Scholar]
  22. Rhoads D. B., Laimins L., Epstein W. 1978; Functional organization of the kdp genes of Escherichia coli K-12. Journal of Bacteriology 135:445–452
    [Google Scholar]
  23. Ross G. W., Callaghan C. H.O. 1975; Lactamase assays.. Methods in Enzymology 43:69–85
    [Google Scholar]
  24. Sancar A., Hack A. M., Rupp W. D. 1979; Simple method for identification of plasmid-coded proteins.. Journal of Bacteriology 137:692–693
    [Google Scholar]
  25. Smith M. W., Neidhardt F. C. 1983; 2-Oxoacid dehydrogenase complexes of Escherichia coli: cellular amounts and patterns of synthesis.. Journal of Bacteriology 156:81–88
    [Google Scholar]
  26. Spencer M. E., Guest J. R. 1973; Isolation and properties of fumarate reductase mutants of Escherichia coli.. Journal of Bacteriology 114:563–570
    [Google Scholar]
  27. SPENCER M. E., GUEST J. R. 1982; Molecular cloning of four tricarboxylic acid cycle genes of Escherichia coli.. Journal of Bacteriology 151:542–552
    [Google Scholar]
  28. Spencer M. E., Guest J. R. 1985; Transcription analysis of the sucAB, aceEF and Ipd genes of Escherichia coli.. Molecular and General Genetics 200:145–154
    [Google Scholar]
  29. Spencer M. E., Darlison M. G., Stephens P. E., Duckenfield I. K., Guest J. R. 1984; Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase.. European Journal of Biochemistry 141:361–374
    [Google Scholar]
  30. Stephens P. E., Darlison M. G., Lewis H. M., Guest J. R. 1983; The pyruvate dehydrogenase complex of Escherichia coli K12 Nucleotide sequence encoding the pyruvate dehydrogenase component.. European Journal of Biochemistry 133:155162
    [Google Scholar]
  31. Stern M. J., Ames G. F., Smith N. H.L., Robinson C. E., Higgins C. F. 1984; Repetitive extragenic palindromic sequences: a major component of the bacterial genome.. Cell 37:1019–1026
    [Google Scholar]
  32. Vogel H., Bonner D. M. 1956; A convenient growth medium for Escherichia coli and some other microorganisms.. Microbial Genetics Bulletin 13:43–44
    [Google Scholar]
  33. Vogel H. J., Bridger W. A. 1982; A phosphorus 31 nuclear magnetic resonance study of the intermediates of the Escherichia coli succinyl-coenzymeA synthetase reactions.. Journal of Biological Chemistry 257:4834–4842
    [Google Scholar]
  34. Weitzman P. D. J. 1981; Unity and diversity in some bacterial citric acid cycle enzymes.. Advances in Microbial Physiology 22:185–244
    [Google Scholar]
  35. Wood D., Darlison M. G., Wilde R. J., Guest J. R. 1984; Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli.. Biochemical Journal 222:519–534
    [Google Scholar]
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