1887

Abstract

Summary: Glutamine synthetase (GS) (EC 6.3.1.2) was purified from an deletion strain containing the GS structural gene. The apparent of the cloned GS subunit was approximately 60000. This indicates a particle for the undissociated enzyme of 720000, assuming the enzyme is the typical dodecamer. Electron microscopy of purified GS revealed characteristic disc shaped molecules with central holes. The cloned GS was regulatedby Mg or Mn,adenylylation, and nitrogen source but was not affected by feedback modifiers. The GS has a -glutamyltransferase isoactivity point of pH 7.71.

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1986-07-01
2024-05-02
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